{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T05:48:41Z","timestamp":1789019321482,"version":"build-2803163510"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2016,8,15]],"date-time":"2016-08-15T00:00:00Z","timestamp":1471219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100002999","name":"Dutch Ministry of Health, Welfare and Sport","doi-asserted-by":"crossref","award":["V\/150017"],"award-info":[{"award-number":["V\/150017"]}],"id":[{"id":"10.13039\/501100002999","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Garden soils were investigated as reservoirs and potential sources of pathogenic\n            <jats:named-content content-type=\"genus-species\">Legionella<\/jats:named-content>\n            bacteria.\n            <jats:named-content content-type=\"genus-species\">Legionella<\/jats:named-content>\n            bacteria were detected in 22 of 177 garden soil samples (12%) by amoebal coculture. Of these 22\n            <jats:named-content content-type=\"genus-species\">Legionella<\/jats:named-content>\n            -positive soil samples, seven contained\n            <jats:named-content content-type=\"genus-species\">Legionella pneumophila<\/jats:named-content>\n            . Several other species were found, including the pathogenic\n            <jats:named-content content-type=\"genus-species\">Legionella longbeachae<\/jats:named-content>\n            (4 gardens) and\n            <jats:named-content content-type=\"genus-species\">Legionella sainthelensi<\/jats:named-content>\n            (9 gardens). The\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            isolates comprised 15 different sequence types (STs), and eight of these STs were previously isolated from patients according to the European Working Group for\n            <jats:named-content content-type=\"genus-species\">Legionella<\/jats:named-content>\n            Infections (EWGLI) database. Six gardens that were found to be positive for\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            were resampled after several months, and in three gardens,\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            was again isolated. One of these gardens was resampled four times throughout the year and was found to be positive for\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            on all occasions.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            Tracking the source of infection for sporadic cases of Legionnaires' disease (LD) has proven to be hard.\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            ST47, the sequence type that is most frequently isolated from LD patients in the Netherlands, is rarely found in potential environmental sources. As\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            ST47 was previously isolated from a garden soil sample during an outbreak investigation, garden soils were investigated as reservoirs and potential sources of pathogenic\n            <jats:named-content content-type=\"genus-species\">Legionella<\/jats:named-content>\n            bacteria. The detection of viable, clinically relevant\n            <jats:named-content content-type=\"genus-species\">Legionella<\/jats:named-content>\n            strains indicates that garden soil is a potential source of\n            <jats:named-content content-type=\"genus-species\">Legionella<\/jats:named-content>\n            bacteria, and future research should assess the public health implication of the presence of\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            in garden soil.\n          <\/jats:p>","DOI":"10.1128\/aem.00595-16","type":"journal-article","created":{"date-parts":[[2016,6,18]],"date-time":"2016-06-18T02:23:51Z","timestamp":1466216631000},"page":"5125-5131","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Presence and Persistence of Viable, Clinically Relevant Legionella pneumophila Bacteria in Garden Soil in the Netherlands"],"prefix":"10.1128","volume":"82","author":[{"given":"E.","family":"van Heijnsbergen","sequence":"first","affiliation":[{"name":"National Institute for Public Health and the Environment, Bilthoven, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"van Deursen","sequence":"additional","affiliation":[{"name":"National Institute for Public Health and the Environment, Bilthoven, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Bouwknegt","sequence":"additional","affiliation":[{"name":"National Institute for Public Health and the Environment, Bilthoven, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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