{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T02:57:46Z","timestamp":1771210666107,"version":"3.50.1"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2009,8,15]],"date-time":"2009-08-15T00:00:00Z","timestamp":1250294400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2009,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Most studies of bacterial denitrification have used nitrate (NO\n            <jats:sub>3<\/jats:sub>\n            <jats:sup>\u2212<\/jats:sup>\n            ) as the first electron acceptor, whereas relatively less is understood about nitrite (NO\n            <jats:sub>2<\/jats:sub>\n            <jats:sup>\u2212<\/jats:sup>\n            ) denitrification. We isolated novel bacteria that proliferated in the presence of high levels of NO\n            <jats:sub>2<\/jats:sub>\n            <jats:sup>\u2212<\/jats:sup>\n            (72 mM). Strain YD50.2, among several isolates, was taxonomically positioned within the \u03b1 subclass of\n            <jats:italic>Proteobacteria<\/jats:italic>\n            and identified as\n            <jats:italic>Ochrobactrum anthropi<\/jats:italic>\n            YD50.2. This strain denitrified NO\n            <jats:sub>2<\/jats:sub>\n            <jats:sup>\u2212<\/jats:sup>\n            , as well as NO\n            <jats:sub>3<\/jats:sub>\n            <jats:sup>\u2212<\/jats:sup>\n            . The gene clusters for denitrification (\n            <jats:italic>nar<\/jats:italic>\n            ,\n            <jats:italic>nir<\/jats:italic>\n            ,\n            <jats:italic>nor<\/jats:italic>\n            , and\n            <jats:italic>nos<\/jats:italic>\n            ) were cloned from\n            <jats:italic>O. anthropi<\/jats:italic>\n            YD50.2, in which the\n            <jats:italic>nir<\/jats:italic>\n            and\n            <jats:italic>nor<\/jats:italic>\n            operons were linked. We confirmed that\n            <jats:italic>nirK<\/jats:italic>\n            in the\n            <jats:italic>nir<\/jats:italic>\n            -\n            <jats:italic>nor<\/jats:italic>\n            operon produced a functional NO\n            <jats:sub>2<\/jats:sub>\n            <jats:sup>\u2212<\/jats:sup>\n            reductase containing copper that was involved in bacterial NO\n            <jats:sub>2<\/jats:sub>\n            <jats:sup>\u2212<\/jats:sup>\n            reduction. The strain denitrified up to 40 mM NO\n            <jats:sub>2<\/jats:sub>\n            <jats:sup>\u2212<\/jats:sup>\n            to dinitrogen under anaerobic conditions in which other denitrifiers or NO\n            <jats:sub>3<\/jats:sub>\n            <jats:sup>\u2212<\/jats:sup>\n            reducers such as\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            and\n            <jats:italic>Ralstonia eutropha<\/jats:italic>\n            and nitrate-respiring\n            <jats:italic>Escherichia coli<\/jats:italic>\n            neither proliferated nor reduced NO\n            <jats:sub>2<\/jats:sub>\n            <jats:sup>\u2212<\/jats:sup>\n            . Under nondenitrifying aerobic conditions,\n            <jats:italic>O. anthropi<\/jats:italic>\n            YD50.2 and its type strain ATCC 49188\n            <jats:sup>T<\/jats:sup>\n            proliferated even in the presence of higher levels of NO\n            <jats:sub>2<\/jats:sub>\n            <jats:sup>\u2212<\/jats:sup>\n            (100 mM), and both were considerably more resistant to acidic NO\n            <jats:sub>2<\/jats:sub>\n            <jats:sup>\u2212<\/jats:sup>\n            than were the other strains noted above. These results indicated that\n            <jats:italic>O. anthropi<\/jats:italic>\n            YD50.2 is a novel denitrifier that has evolved reactive nitrogen oxide tolerance mechanisms.\n          <\/jats:p>","DOI":"10.1128\/aem.00604-09","type":"journal-article","created":{"date-parts":[[2009,6,20]],"date-time":"2009-06-20T01:46:03Z","timestamp":1245462363000},"page":"5186-5194","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Novel Denitrifying Bacterium\n            <i>Ochrobactrum anthropi<\/i>\n            YD50.2 Tolerates High Levels of Reactive Nitrogen Oxides"],"prefix":"10.1128","volume":"75","author":[{"given":"Yuki","family":"Doi","sequence":"first","affiliation":[{"name":"Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoki","family":"Takaya","sequence":"additional","affiliation":[{"name":"Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noboru","family":"Takizawa","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Okayama University of Science, Okayama-Shi, Okayama 700-0005, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.11.2987-2993.2002"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.18.6025-6031.2004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.9.2802-2806.1999"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00283030"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.10.3769-3775.1998"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)54420-3"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/22.5.845"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.21.6769-6777.1997"},{"key":"e_1_3_2_10_2","first-page":"17","year":"1981","unstructured":"Crutzen, P. J. 1981. Atmospheric chemical processes of the oxides of nitrogen, including nitrous oxide, p. 17-44. In C. C. Delwiche (ed.), Denitrification, nitrification, and atmospheric nitrous oxide. John Wiley & Sons, Inc., New York, NY.","journal-title":"Denitrification, nitrification, and atmospheric nitrous oxide."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.0592r.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1389-1723(03)80200-1"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1004"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00871634"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.4.1615-1619.2006"},{"key":"e_1_3_2_16_2","first-page":"88","volume":"1996","year":"1996","unstructured":"H\u00e4rtig, E., and W. G. Zumft. 1996. Nitrate and nitrite response of denitrification genes studied by mRNA analysis. BIOspectrum1996:88.","journal-title":"BIOspectrum"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.12.3658-3665.1999"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-9-2505"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.1.235-242.1997"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.46.1.43-70.1982"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(03)00089-7"},{"key":"e_1_3_2_23_2","first-page":"983","volume":"3","year":"1951","unstructured":"Nicholas, D. J. D., and A. Nason. 1951. Determination of nitrate and nitrite. Methods Enzymol.3:983-984.","journal-title":"Methods Enzymol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-139-4-725"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-8524(03)00088-9"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4781(02)00420-7"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4781(00)00286-4"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02157.x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0330176"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.18.10449"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.1996.tb00561.x"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2004.04.035"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.200600036"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01446-06"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99130-1"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.24.7247-7256.2003"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1354(00)00471-1"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1021\/tx9600311"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-005-0157-9"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.8.2516-2526.2001"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.12.3606-3613.2001"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(85)90120-9"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.1.250-254.1993"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.12.3606-3609.1995"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00295-2"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1139\/w05-010"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1994.tb19962.x"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.61.4.533-616.1997"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00604-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00604-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T16:56:18Z","timestamp":1645635378000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.00604-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,8,15]]},"references-count":47,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2009,8,15]]}},"alternative-id":["10.1128\/AEM.00604-09"],"URL":"https:\/\/doi.org\/10.1128\/aem.00604-09","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,8,15]]},"assertion":[{"value":"2009-03-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-06-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-08-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}