{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T06:59:39Z","timestamp":1781506779009,"version":"3.54.1"},"reference-count":74,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2016,6,13]],"date-time":"2016-06-13T00:00:00Z","timestamp":1465776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000879","name":"Alfred P. Sloan Foundation","doi-asserted-by":"publisher","award":["SPO-036450-003"],"award-info":[{"award-number":["SPO-036450-003"]}],"id":[{"id":"10.13039\/100000879","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Given that epiphytic microbes are often found in large population sizes on plants, we tested the hypothesis that plants are quantitatively important local sources of airborne microorganisms. The abundance of microbial communities, determined by quantifying bacterial 16S RNA genes and the fungal internal transcribed spacer (ITS) region, in air collected directly above vegetation was 2- to 10-fold higher than that in air collected simultaneously in an adjacent nonvegetated area 50 m upwind. Nonmetric multidimensional scaling revealed that the composition of airborne bacteria in upwind air samples grouped separately from that of downwind air samples, while communities on plants and downwind air could not be distinguished. In contrast, fungal taxa in air samples were more similar to each other than to the fungal epiphytes. A source-tracking algorithm revealed that up to 50% of airborne bacteria in downwind air samples were presumably of local plant origin. The difference in the proportional abundances of a given operational taxonomic unit (OTU) between downwind and upwind air when regressed against the proportional representation of this OTU on the plant yielded a positive slope for both bacteria and fungi, indicating that those taxa that were most abundant on plants proportionally contributed more to downwind air. Epiphytic fungi were less of a determinant of the microbiological distinctiveness of downwind air and upwind air than epiphytic bacteria. Emigration of epiphytic bacteria and, to a lesser extent, fungi, from plants can thus influence the microbial composition of nearby air, a finding that has important implications for surrounding ecosystems, including the built environment into which outdoor air can penetrate.<\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            This paper addresses the poorly understood role of bacterial and fungal epiphytes, the inhabitants of the aboveground plant parts, in the composition of airborne microbes in outdoor air. It is widely held that epiphytes contribute to atmospheric microbial assemblages, but much of what we know is limited to qualitative assessments. Elucidating the sources of microbes in outdoor air can inform basic biological processes seen in airborne communities (e.g., dispersal and biogeographical patterns). Furthermore, given the considerable contribution of outdoor air to microbial communities found within indoor environments, the understanding of plants as sources of airborne microbes in outdoor air might contribute to our understanding of indoor air quality. With an experimental design developed to minimize the likelihood of other-than-local plant sources contributing to the composition of airborne microbes, we provide direct evidence that plants are quantitatively important local sources of airborne microorganisms, with implications for the surrounding ecosystems.\n          <\/jats:p>","DOI":"10.1128\/aem.00610-16","type":"journal-article","created":{"date-parts":[[2016,4,23]],"date-time":"2016-04-23T03:54:01Z","timestamp":1461383641000},"page":"3822-3833","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":149,"title":["Contribution of Vegetation to the Microbial Composition of Nearby Outdoor Air"],"prefix":"10.1128","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8692-9843","authenticated-orcid":false,"given":"Despoina S.","family":"Lymperopoulou","sequence":"first","affiliation":[{"name":"Department of Plant &amp; Microbial Biology, University of California, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel I.","family":"Adams","sequence":"additional","affiliation":[{"name":"Department of Plant &amp; Microbial Biology, University of California, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven E.","family":"Lindow","sequence":"additional","affiliation":[{"name":"Department of Plant &amp; Microbial Biology, University of California, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.38.1.145"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.4.1875-1883.2003"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-1669(02)00313-0"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2910"},{"key":"e_1_3_3_6_2","first-page":"69","volume-title":"Phyllosphere microbiology","author":"Upper CD","year":"2002","unstructured":"Upper CD, Hirano SS, Lindow SE, Hecht-Poinar EI, Elliott VJ. 2002. Revisiting the roles of immigration and growth in the development of populations of Pseudomonas syringae in the phyllosphere, p 69\u201379. In Lindow SE, Hecht-Poinar EI, Elliott VJ (ed), Phyllosphere microbiology. APS Press, St. Paul, MN."},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.50.5.1229-1232.1985"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-409-90199-3.50011-5"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.44.5.1059-1063.1982"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1080\/00173138709428909"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/00173139209427826"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.47.2.430-432.1984"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.4.1492-1496.1995"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002489900046"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007694618888"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.8.2978-2987.1996"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00442-011-2138-2"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-009-9495-y"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1216057111"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-010-9742-2"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00888-12"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05565-11"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2012.32"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05498-11"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2010.167"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00447-09"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/es402970s"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1420815112"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2013.28"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2015.1139"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-014-0066-1"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12889"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-014-0087-z"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000080107"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090234"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2634"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01541-09"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1219"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2010.02193.x"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr381"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02810-10"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2604"},{"key":"e_1_3_3_43_2","doi-asserted-by":"crossref","unstructured":"Martin M. 2011. Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet.journal 17:10\u201312. http:\/\/journal.embnet.org\/index.php\/embnetjournal\/article\/view\/200\/479.","DOI":"10.14806\/ej.17.1.200"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2009.03160.x"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsoil.2010.04.003"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.2000.0324"},{"key":"e_1_3_3_48_2","volume-title":"R: a language and environment for statistical computing","author":"R Development Core Team","year":"2008","unstructured":"R Development Core Team. 2008. R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http:\/\/www.R-project.org."},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061217"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-98141-3"},{"key":"e_1_3_3_51_2","unstructured":"Oksanen J Blanchet FG Kindt R Legendre P Minchin PR O'Hara RB Simpson GL Solymos P Stevens MHH Wagner H. 2016. vegan: Community Ecology Package. R package version 2.3-3. http:\/\/CRAN.R-project.org\/package=vegan."},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0132783"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1650"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6968.12225"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608255104"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.01926.x"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1134\/S0026261710060160"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1201\/9780203302934"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.3920\/978-90-8686-722-6"},{"key":"e_1_3_3_60_2","volume-title":"Introduction to food-borne fungi","author":"Samson RA","year":"1995","unstructured":"Samson RA, Hoekstra ES, Frisvald JC, Filtenborg O. 1995. Introduction to food-borne fungi, 4th ed. Centraalbureau voor Schimmelcultures, Baarn-Delft, The Netherlands.","edition":"4"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.py.30.090192.001301"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.3402\/tellusb.v64i0.15598"},{"key":"e_1_3_3_63_2","volume-title":"The microbiology of the atmosphere","author":"Gregory PH","year":"1973","unstructured":"Gregory PH. 1973. The microbiology of the atmosphere, 2nd ed. Leonard Hill, London, United Kingdom.","edition":"2"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.1.346-355.2004"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.9.5484-5493.2005"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.061629598"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.5194\/bg-9-803-2012"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2010.9"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1111\/ina.12047"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0128022"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.4.1743-1753.2002"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-050312-120106"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2010.02258.x"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000454107"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2014.10.086"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00610-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00610-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T18:13:00Z","timestamp":1645639980000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.00610-16"}},"subtitle":[],"editor":[{"given":"F. E.","family":"L\u00f6ffler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,7]]},"references-count":74,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2016,7]]}},"alternative-id":["10.1128\/AEM.00610-16"],"URL":"https:\/\/doi.org\/10.1128\/aem.00610-16","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7]]},"assertion":[{"value":"2016-02-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-04-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-06-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}