{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T11:13:37Z","timestamp":1780485217021,"version":"3.54.1"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2016,9,15]],"date-time":"2016-09-15T00:00:00Z","timestamp":1473897600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2016,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Spinosad, a highly effective insecticide, has an excellent environmental and mammalian toxicological profile. Global market demand for spinosad is huge and growing. However, after much effort, there has been almost no improvement in the spinosad yield from the original producer,\n            <jats:named-content content-type=\"genus-species\">Saccharopolyspora spinosa<\/jats:named-content>\n            . Here, we report the heterologous expression of spinosad using\n            <jats:named-content content-type=\"genus-species\">Saccharopolyspora erythraea<\/jats:named-content>\n            as a host. The native erythromycin polyketide synthase (PKS) genes in\n            <jats:named-content content-type=\"genus-species\">S. erythraea<\/jats:named-content>\n            were replaced by the assembled spinosad gene cluster through iterative recombination. The production of spinosad could be detected in the recombinant strains containing the whole biosynthesis gene cluster. Both metabolic engineering and UV mutagenesis were applied to further improve the yield of spinosad. The final strain, AT-ES04PS-3007, which could produce spinosad with a titer of 830 mg\/liter, has significant potential in industrial applications.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            This work provides an innovative and promising way to improve the industrial production of spinosad. At the same time, it also describes a successful method of heterologous expression for target metabolites of interest by replacing large gene clusters.\n          <\/jats:p>","DOI":"10.1128\/aem.00618-16","type":"journal-article","created":{"date-parts":[[2016,7,12]],"date-time":"2016-07-12T06:51:37Z","timestamp":1468306297000},"page":"5603-5611","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["High Level of Spinosad Production in the Heterologous Host Saccharopolyspora erythraea"],"prefix":"10.1128","volume":"82","author":[{"given":"Jun","family":"Huang","sequence":"first","affiliation":[{"name":"Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, China"},{"name":"Central Research Institute, Zhejiang Hisun Pharmaceutical Co., Ltd., Taizhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Yu","sequence":"additional","affiliation":[{"name":"Central Research Institute, Zhejiang Hisun Pharmaceutical Co., Ltd., Taizhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei-Hong","family":"Li","sequence":"additional","affiliation":[{"name":"Central Research Institute, Zhejiang Hisun Pharmaceutical Co., Ltd., Taizhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Dong","family":"Wang","sequence":"additional","affiliation":[{"name":"Central Research Institute, Zhejiang Hisun Pharmaceutical Co., Ltd., Taizhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Bai","sequence":"additional","affiliation":[{"name":"Central Research Institute, Zhejiang Hisun Pharmaceutical Co., Ltd., Taizhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Central Research Institute, Zhejiang Hisun Pharmaceutical Co., Ltd., Taizhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Guo","family":"Zheng","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(00)93474-9"},{"key":"e_1_3_3_3_2","first-page":"1","article-title":"The discovery of Saccharopolyspora spinosa and a new class of insect control products","volume":"52","author":"Thompson GD","year":"1997","unstructured":"Thompson GD, Michel KH, Yao RC, Mynderse JS, Mosburg CT, Worden TV, Chio EH, Sparks TC, Hutchins SH. 1997. 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