{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:46:16Z","timestamp":1791942376930,"version":"4.3.4"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We report on the characterization of a group of seven novel\n            <jats:italic>Brucella<\/jats:italic>\n            strains isolated in 1964 from three native rodent species in North Queensland, Australia, during a survey of wild animals. The strains were initially reported to be\n            <jats:italic>Brucella suis<\/jats:italic>\n            biovar 3 on the basis of microbiological test results. Our results indicated that the rodent strains had microbiological traits distinct from those of\n            <jats:italic>B. suis<\/jats:italic>\n            biovar 3 and all other\n            <jats:italic>Brucella<\/jats:italic>\n            spp. To reinvestigate these rodent strains, we sequenced the 16S rRNA,\n            <jats:italic>recA<\/jats:italic>\n            , and\n            <jats:italic>rpoB<\/jats:italic>\n            genes and nine housekeeping genes and also performed multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA). The rodent strains have a unique 16S rRNA gene sequence compared to the sequences of the classical\n            <jats:italic>Brucella<\/jats:italic>\n            spp. Sequence analysis of the\n            <jats:italic>recA<\/jats:italic>\n            ,\n            <jats:italic>rpoB<\/jats:italic>\n            , and nine housekeeping genes reveals that the rodent strains are genetically identical to each other at these loci and divergent from any of the currently described\n            <jats:italic>Brucella<\/jats:italic>\n            sequence types. However, all seven of the rodent strains do exhibit distinctive allelic MLVA profiles, although none demonstrated an amplicon for VNTR 07, whereas the other\n            <jats:italic>Brucella<\/jats:italic>\n            spp. did. Phylogenetic analysis of the MLVA data reveals that the rodent strains form a distinct clade separate from the classical\n            <jats:italic>Brucella<\/jats:italic>\n            spp. Furthermore, whole-genome sequence comparison using the maximal unique exact matches index (MUMi) demonstrated a high degree of relatedness of one of the seven rodent\n            <jats:italic>Brucella<\/jats:italic>\n            strains (strain NF 2653) to another Australian rodent\n            <jats:italic>Brucella<\/jats:italic>\n            strain (strain 83-13). Our findings strongly suggest that this group of\n            <jats:italic>Brucella<\/jats:italic>\n            strains isolated from wild Australian rodents defines a new species in the\n            <jats:italic>Brucella<\/jats:italic>\n            genus.\n          <\/jats:p>","DOI":"10.1128\/aem.00620-10","type":"journal-article","created":{"date-parts":[[2010,7,17]],"date-time":"2010-07-17T02:01:16Z","timestamp":1279332076000},"page":"5837-5845","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":100,"title":["Characterization of Novel\n            <i>Brucella<\/i>\n            Strains Originating from Wild Native Rodent Species in North Queensland, Australia"],"prefix":"10.1128","volume":"76","author":[{"given":"Rebekah V.","family":"Tiller","sequence":"first","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jay E.","family":"Gee","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Frace","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trevor K.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Australian Animal Health Laboratory, Geelong, Victoria 3220, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joao C.","family":"Setubal","sequence":"additional","affiliation":[{"name":"Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex R.","family":"Hoffmaster","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barun K.","family":"De","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","first-page":"1","year":"1975","unstructured":"Alton, G. 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