{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:42:18Z","timestamp":1791182538019,"version":"4.1.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T00:00:00Z","timestamp":1372636800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>We investigated the prevalence of<jats:named-content content-type=\"genus-species\">Legionella<\/jats:named-content>species isolated from puddles on asphalt roads. In addition, we carried out sequence-based typing (SBT) analysis on the genetic relationship between<jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>serogroup 1 (SG 1) isolates from puddles and from stock strains previously obtained from sputum specimens and public baths. Sixty-nine water samples were collected from puddles on roads at 6 fixed locations.<jats:named-content content-type=\"genus-species\">Legionella<\/jats:named-content>species were detected in 33 samples (47.8%) regardless of season. Among the 325 isolates from puddles, strains of<jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>SG 1, a major causative agent of Legionnaires' disease, were the most frequently isolated (<jats:italic>n<\/jats:italic>= 62, 19.1%). Sixty-two isolates of<jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>SG 1 from puddles were classified into 36 sequence types (STs) by SBT. ST120 and ST48 were identified as major STs. Environmental ST120 strains from puddles were found for the first time in this study. Among the 14 STs of the clinical isolates (<jats:italic>n<\/jats:italic>= 19), 4 STs (<jats:italic>n<\/jats:italic>= 6, 31.6%), including ST120, were also detected in isolates from puddles on roads, and the sources of infection in these cases remained unclear. The<jats:italic>lag-1<\/jats:italic>gene, a tentative marker for clinical isolates, was prevalent in puddle isolates (61.3%). Our findings suggest that puddles on asphalt roads serve as potential reservoirs for<jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>in the environment.<\/jats:p>","DOI":"10.1128\/aem.00637-13","type":"journal-article","created":{"date-parts":[[2013,4,20]],"date-time":"2013-04-20T03:18:42Z","timestamp":1366427922000},"page":"3959-3966","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Close Genetic Relationship between Legionella pneumophila Serogroup 1 Isolates from Sputum Specimens and Puddles on Roads, as Determined by Sequence-Based Typing"],"prefix":"10.1128","volume":"79","author":[{"given":"Jun-ichi","family":"Kanatani","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Toyama Institute of Health, Nakataikoyama, Imizu-shi, Toyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junko","family":"Isobe","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Toyama Institute of Health, Nakataikoyama, Imizu-shi, Toyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiko","family":"Kimata","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Toyama Institute of Health, Nakataikoyama, Imizu-shi, Toyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoko","family":"Shima","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Toyama Institute of Health, Nakataikoyama, Imizu-shi, Toyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miwako","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Toyama Institute of Health, Nakataikoyama, Imizu-shi, Toyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumiaki","family":"Kura","sequence":"additional","affiliation":[{"name":"Department of Bacteriology I, National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsutaro","family":"Sata","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Toyama Institute of Health, Nakataikoyama, Imizu-shi, Toyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanori","family":"Watahiki","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Toyama Institute of Health, Nakataikoyama, Imizu-shi, Toyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197712012972201"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-94-2-195"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a116895"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.2169\/internalmedicine.41.859"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/22.2.257"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/146.4.460"},{"key":"e_1_3_2_8_2","unstructured":"EuzebyJP. 2013. 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