{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T08:36:28Z","timestamp":1648715788019},"reference-count":22,"publisher":"American Society for Microbiology","issue":"15","license":[{"start":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T00:00:00Z","timestamp":1343779200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Research on\n            <jats:named-content content-type=\"genus-species\">Legionella pneumophila<\/jats:named-content>\n            , the causative agent of Legionnaires' disease, has been hampered due to the lack of selectable markers for genetic manipulation. We report the construction of a mutant strain of\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            lacking\n            <jats:italic>loxA<\/jats:italic>\n            , a chromosomally encoded \u03b2-lactamase, that has enhanced sensitivity to ampicillin. Also described are a method for converting\n            <jats:named-content content-type=\"genus-species\">Legionella<\/jats:named-content>\n            strains to ampicillin sensitivity and conditions for utilizing\n            <jats:italic>bla<\/jats:italic>\n            as a selectable marker.\n          <\/jats:p>","DOI":"10.1128\/aem.00669-12","type":"journal-article","created":{"date-parts":[[2012,5,26]],"date-time":"2012-05-26T04:48:54Z","timestamp":1338007734000},"page":"5457-5459","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Reclamation of Ampicillin Sensitivity for the Genetic Manipulation of Legionella pneumophila"],"prefix":"10.1128","volume":"78","author":[{"given":"Molly C.","family":"Sutherland","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph P.","family":"Vogel","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.4.1320-1329.1993"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkf135"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/26.1.45"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01092.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.6.1538-1543.2000"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2007.09.010"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.18.3.403"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.3.506-526.2002"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.12.3509-3516.2001"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.16.5.561"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.29.5.925"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.4.6.995"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.2169\/internalmedicine.46.6006"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki391"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.13.3.419"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.151.1.216-221.1982"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197712012972202"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/22.4.457"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.128.3.1401"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.12.5361-5373.1993"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.12.3814-3825.2004"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/23.1.43"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00669-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00669-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T01:36:11Z","timestamp":1645580171000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.00669-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8]]},"references-count":22,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2012,8]]}},"alternative-id":["10.1128\/AEM.00669-12"],"URL":"https:\/\/doi.org\/10.1128\/aem.00669-12","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8]]},"assertion":[{"value":"2012-03-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-05-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-07-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}