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Here, we constructed a catalog of 2,474 nonredundant feline gut microbial genomes and integrated serum metabolomic data to verify BA transformation alterations. The results revealed that the feline gut microbiome harbored widespread genetic potential for BA transformation but lacked a complete 7\u03b1-dehydroxylation pathway due to the absence of the key gene\n                      <jats:italic toggle=\"yes\">baiE<\/jats:italic>\n                      . The BA transformation-related genomes (2,045 in total) were predominantly from the phyla Bacillota_A and Actinomycetota, among which only 37 encoded\n                      <jats:italic toggle=\"yes\">baiB<\/jats:italic>\n                      , all belonging to Bacillota_A. The distribution of BA transformation-related genes varied across intestinal regions: genes encoding 7\u03b1-HSDH were primarily enriched in the small intestine, whereas genes encoding 3\u03b1-HSDH,\n                      <jats:italic toggle=\"yes\">baiCD<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">baiH<\/jats:italic>\n                      were more abundant in the large intestine. Additionally, the abundance of genes encoding BSH and 3\u03b1-HSDH increased significantly in the small intestine on day 3 post-infection, accompanied by increases in the phylum Bacillota_C and genera such as\n                      <jats:italic toggle=\"yes\">Blautia_A<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Enterococcus_E<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Ligilactobacillus<\/jats:italic>\n                      . Serum metabolomics revealed a significant increase in cholesterol levels post-infection, supporting the impact of\n                      <jats:italic toggle=\"yes\">T. gondii<\/jats:italic>\n                      infection on intestinal BA transformation. These findings illustrated that the feline gut microbiota played an important role in BA transformation and that\n                      <jats:italic toggle=\"yes\">T. gondii<\/jats:italic>\n                      infection disrupted the microbial potential for secondary BA transformation. This study provided new insights into gut microbiota-associated metabolic perturbations during feline toxoplasmosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Bile acid (BA) transformation plays a critical role in host metabolism and immune regulation. Although studies on BA transformation are increasing, the capacity for BA transformation within the feline gut microbiota and the impact of\n                      <jats:italic toggle=\"yes\">Toxoplasma gondii<\/jats:italic>\n                      infection on this capacity remain unclear. To bridge this gap, we constructed a catalog of 2,474 nonredundant feline gut microbial genomes and integrated serum metabolomic data to verify BA transformation alterations. Our findings revealed that the feline gut microbiome lacked a complete 7\u03b1-dehydroxylation pathway, and the specific functions involved in BA transformation may differ between the small and large intestines. Furthermore, integrated metagenomic and serum metabolomic analyses suggested that\n                      <jats:italic toggle=\"yes\">T. gondii<\/jats:italic>\n                      infection disrupted BA transformation capacity in the small intestine. This study provided new insights into gut microbiota-associated metabolic perturbations during feline toxoplasmosis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aem.00674-26","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T15:23:51Z","timestamp":1789140231000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["<i>Toxoplasma gondii<\/i>\n                    infection disrupts secondary bile acid transformation in feline gut microbiota"],"prefix":"10.1128","volume":"92","author":[{"given":"Lu-Yao","family":"Tang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051qwcj72","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Veterinary Medicine, Qingdao Agricultural University","place":["Qingdao, 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