{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T13:28:47Z","timestamp":1787146127562,"version":"build-2736575974"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2021YFD1800403"],"award-info":[{"award-number":["2021YFD1800403"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013314","name":"111 Project","doi-asserted-by":"crossref","award":["D18007"],"award-info":[{"award-number":["D18007"]}],"id":[{"id":"10.13039\/501100013314","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Priority Academic Program Development of Jiangsu Higher Education Institutions","award":["PAPD"],"award-info":[{"award-number":["PAPD"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2026,8,19]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Bovine tuberculosis (bTB), caused by\n                      <jats:italic toggle=\"yes\">Mycobacterium bovis<\/jats:italic>\n                      , causes major economic losses in the global livestock industry and threatens public health. Macrophages are essential for pathogen clearance, yet serve as the primary intracellular niche for mycobacterial survival. These characteristics emphasize the importance of identifying host factors that regulate macrophage functions during infection. To systematically uncover such factors, we constructed a genome-wide CRISPR-KO library in Bomac cells (the bovine macrophage cell line) and performed iterative infection screening under high selective pressure. Here, the host protein SH3PXD2B was identified as a key host factor to promote mycobacterial invasion: SH3PXD2B was significantly enriched in the screening.\n                      <jats:italic toggle=\"yes\">M. bovis<\/jats:italic>\n                      infection significantly increases the expression of SH3PXD2B. The phagocytic activity of macrophages is enhanced in an SH3PXD2B-dependent manner, and the high expression of SH3PXD2B inhibits the migration of macrophages, causing macrophages to remain at the site of infection and promoting the entry of more mycobacteria into macrophages to establish infection. Further results indicate that macrophage migration inhibitory factor is positively regulated by SH3PXD2B and plays an important role in SH3PXD2B-mediated macrophage migration and phagocytic activity. These results indicate that SH3PXD2B is a critical host factor manipulated by\n                      <jats:italic toggle=\"yes\">M. bovis<\/jats:italic>\n                      to promote infection. It provides a new potential target for genetic breeding against bTB.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        There is a complex interaction between\n                        <jats:italic toggle=\"yes\">Mycobacterium bovis<\/jats:italic>\n                        and the host, which has an important impact on the occurrence and development of bTB. However, the host gene regulatory network for\n                        <jats:italic toggle=\"yes\">M. bovis<\/jats:italic>\n                        infection is still incomplete, and the specific genes that play a key role in the infection process are still unclear. Bovine tuberculosis remains a major economic and public health burden worldwide. This study identifies SH3PXD2B as a novel host factor that is exploited by\n                        <jats:italic toggle=\"yes\">M. bovis<\/jats:italic>\n                        to promote macrophage phagocytosis and inhibit migration, thereby facilitating bacterial invasion. These findings reveal a new pathogenic strategy and suggest that SH3PXD2B may represent a potential target for host-directed therapy and genetic breeding against bTB.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/aem.00675-26","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T13:01:06Z","timestamp":1784293266000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["SH3PXD2B is the critical host factor promoting the entry of mycobacteria"],"prefix":"10.1128","volume":"92","author":[{"given":"Donghui","family":"Liu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Jiangsu Interdisciplinary Center for Zoonoses and Biosafety, Yangzhou University","place":["Yangzhou, China"]},{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality of Ministry of Agriculture and Rural Affairs, Yangzhou University","place":["Yangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Jiangsu Interdisciplinary Center for Zoonoses and Biosafety, Yangzhou University","place":["Yangzhou, China"]},{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality of Ministry of Agriculture and Rural Affairs, Yangzhou University","place":["Yangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Sheng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Jiangsu Interdisciplinary Center for Zoonoses and Biosafety, Yangzhou University","place":["Yangzhou, China"]},{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality of Ministry of Agriculture and Rural Affairs, Yangzhou University","place":["Yangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengzhong","family":"Xu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Jiangsu Interdisciplinary Center for Zoonoses and Biosafety, Yangzhou University","place":["Yangzhou, China"]},{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality of Ministry of Agriculture and Rural Affairs, Yangzhou University","place":["Yangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3030-4251","authenticated-orcid":true,"given":"Chengkun","family":"Zheng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Jiangsu Interdisciplinary Center for Zoonoses and Biosafety, Yangzhou University","place":["Yangzhou, China"]},{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality of Ministry of Agriculture and Rural Affairs, Yangzhou University","place":["Yangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingyu","family":"Chen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/023b72294","id-type":"ROR","asserted-by":"publisher"}],"name":"National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7460-8356","authenticated-orcid":true,"given":"Aizhen","family":"Guo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/023b72294","id-type":"ROR","asserted-by":"publisher"}],"name":"National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2214-3358","authenticated-orcid":true,"given":"Xin'an","family":"Jiao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Jiangsu Interdisciplinary Center for Zoonoses and Biosafety, Yangzhou University","place":["Yangzhou, China"]},{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality of Ministry of Agriculture and Rural Affairs, Yangzhou University","place":["Yangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9736-8180","authenticated-orcid":true,"given":"Xiang","family":"Chen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Jiangsu Interdisciplinary Center for Zoonoses and Biosafety, Yangzhou University","place":["Yangzhou, China"]},{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality of Ministry of Agriculture and Rural Affairs, Yangzhou University","place":["Yangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","volume-title":"Global tuberculosis report 2025","author":"Anonymous","year":"2025","unstructured":"Anonymous. 2025. Global tuberculosis report 2025. World Health Organization, Geneva."},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.02.018"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011871"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuz006"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-020-0502-z"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-023-00374-y"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00772"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005007"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2002.01403.x"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2020.08.010"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2017.016"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3047"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0554-4"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2015.00090"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2014.01.015"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003946"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep27566"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21218117"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.15.4346"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2350-13-104"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2010.01.009"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12920-021-00963-6"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.12954"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13000-021-01090-7"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2023.121792"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12551-012-0081-z"},{"key":"e_1_3_4_28_2","first-page":"403","article-title":"N-WASP promotes invasion and migration of cervical cancer cells through regulating p38 MAPKs signaling pathway","volume":"9","author":"Hou J","year":"2017","unstructured":"Hou J, Yang H, Huang X, Leng X, Zhou F, Xie C, Zhou Y, Xu Y. 2017. N-WASP promotes invasion and migration of cervical cancer cells through regulating p38 MAPKs signaling pathway. Am J Transl Res 9:403\u2013415.","journal-title":"Am J Transl Res"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-49021-3"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023653"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e08-09-0949"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3141"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-014-1731-z"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0000000000000575"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2023.151356"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.19.7522"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1567"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C500243200"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2018.10.001"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2024.101022"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells8111343"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.353"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.02.034"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.8b00084"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.2119\/molmed.2008.00107"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers11040529"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2015.06.008"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.00675-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.00675-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T13:04:35Z","timestamp":1787144675000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.00675-26"}},"subtitle":[],"editor":[{"given":"Christopher A.","family":"Elkins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,8,19]]},"references-count":46,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8,19]]}},"alternative-id":["10.1128\/aem.00675-26"],"URL":"https:\/\/doi.org\/10.1128\/aem.00675-26","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,19]]},"assertion":[{"value":"2026-04-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00675-26"}}