{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:11:35Z","timestamp":1789186295862,"version":"build-2803163510"},"reference-count":100,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T00:00:00Z","timestamp":1750204800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T00:00:00Z","timestamp":1750204800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["1S02022N"],"award-info":[{"award-number":["1S02022N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["1S26823N"],"award-info":[{"award-number":["1S26823N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["S006221N"],"award-info":[{"award-number":["S006221N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2025,6,18]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Ensuring high-quality drinking water remains challenging, as complaints about odors, discoloration, or contamination persist. In Belgium and beyond, traditional flushing is a common curative strategy that involves discharging large water volumes through hydrants while the network remains in use. In some cases, free chlorine (0.5 mg\/L) is added, and consumers are advised not to drink the water. However, flushing can alter water biostability, potentially increasing susceptibility to microbial invasion. This study used a pilot-scale drinking water distribution system with three identical 100 m polyvinyl chloride(PVC) loops (DN 80 mm) to assess the impact of flushing with and without chlorination as practiced in chlorinated networks. Loop 1 was flushed with tap water and sodium hypochlorite (NaOCl), followed by two non-chlorinated flushes, loop 2 was unflushed, and loop 3 underwent three flushes. Biostability was assessed using online flow cytometry, and susceptibility to bacterial invasion (\n                      <jats:italic toggle=\"yes\">Aeromonas media<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Pseudomonas putida<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Serratia fonticola<\/jats:italic>\n                      ) was evaluated in the days following flushing. The water had a 7-day residence time. Results showed that chlorinated flushing promoted microbial regrowth (3.8 \u00d7 10\n                      <jats:sup>5<\/jats:sup>\n                      vs 2.0 \u00d7 10\n                      <jats:sup>5<\/jats:sup>\n                      and 1.6 \u00d7 10\n                      <jats:sup>5<\/jats:sup>\n                      cells\/mL for loops 1, 2, and 3, respectively), primarily of resident\n                      <jats:italic toggle=\"yes\">Sphingopyxis<\/jats:italic>\n                      spp. Biofilm cell densities (~4 \u00d7 10\n                      <jats:sup>6<\/jats:sup>\n                      cells\/cm\n                      <jats:sup>2<\/jats:sup>\n                      ) remained stable across conditions. Bacterial indicators declined over time, with\n                      <jats:italic toggle=\"yes\">P. pudita<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">S. fonticola<\/jats:italic>\n                      surviving longer (&gt;100 hours) than\n                      <jats:italic toggle=\"yes\">A. media<\/jats:italic>\n                      (13 hours). Decay rates were highest in chlorinated loops, likely due to increased microbial competition. For example, the decay constant of\n                      <jats:italic toggle=\"yes\">S. fonticola<\/jats:italic>\n                      at 20\u00b0C was \u22120.082 h\n                      <jats:sup>\u22121<\/jats:sup>\n                      , \u22120.042 h\n                      <jats:sup>\u22121<\/jats:sup>\n                      , and \u22120.027 h\n                      <jats:sup>\u22121<\/jats:sup>\n                      for loops 1, 2, and 3, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>Traditional flushing is used as a curative strategy to solve unwanted quality issues during distribution, yet its impact on microbial biostability remains poorly understood. This study provides critical insights into how traditional flushing, both with and without chlorination, influences microbial regrowth and susceptibility to invasion. Findings reveal that chlorinated flushing promotes the regrowth of resident drinking water bacteria while accelerating the decay of introduced unwanted bacterial indicators, emphasizing the complex trade-off between microbial control and system stability. Understanding these dynamics is essential for optimizing flushing procedures, minimizing unintended consequences, and improving distribution system resilience.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aem.00686-25","type":"journal-article","created":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T09:00:16Z","timestamp":1747126816000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Impact of flushing procedures on drinking water biostability and invasion susceptibility in distribution systems"],"prefix":"10.1128","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8699-0599","authenticated-orcid":true,"given":"Fien","family":"Waegenaar","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00cv9y106","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University","place":["Ghent, Belgium"]},{"id":[{"id":"https:\/\/ror.org\/04s6red60","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Advanced Process Technology for Urban Resource Recovery (CAPTURE)","place":["Ghent, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Pluym","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00cv9y106","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University","place":["Ghent, Belgium"]},{"id":[{"id":"https:\/\/ror.org\/04s6red60","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Advanced Process Technology for Urban Resource Recovery (CAPTURE)","place":["Ghent, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elise","family":"Vermeulen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00cv9y106","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University","place":["Ghent, Belgium"]},{"id":[{"id":"https:\/\/ror.org\/04s6red60","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Advanced Process Technology for Urban Resource Recovery (CAPTURE)","place":["Ghent, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart","family":"De Gusseme","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00cv9y106","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University","place":["Ghent, Belgium"]},{"id":[{"id":"https:\/\/ror.org\/04s6red60","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Advanced Process Technology for Urban Resource Recovery (CAPTURE)","place":["Ghent, Belgium"]},{"name":"Department Innovation Water \u2013 R&D, Farys","place":["Ghent, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7734-3103","authenticated-orcid":true,"given":"Nico","family":"Boon","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00cv9y106","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University","place":["Ghent, Belgium"]},{"id":[{"id":"https:\/\/ror.org\/04s6red60","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Advanced Process Technology for Urban Resource Recovery (CAPTURE)","place":["Ghent, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41545-021-00118-1"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2013.03.002"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00045"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41545-018-0023-9"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2019.115353"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0128825"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0096354"},{"key":"e_1_3_4_9_2","first-page":"10","article-title":"Comparison of the bacterial dynamics in various French distribution systems","volume":"44","author":"Servais P","year":"1995","unstructured":"Servais P, Laurent P, Randon G. 1995. Comparison of the bacterial dynamics in various French distribution systems. Aqua 44:10\u201317.","journal-title":"Aqua"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1061\/(ASCE)WR.1943-5452.0000085"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02205"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2014.01.049"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2016.12.040"},{"key":"e_1_3_4_14_2","volume-title":"Guidelines for drinking-water quality: fourth edition incorporating the first and second addenda","author":"World Health Organization","year":"2022","unstructured":"World Health Organization. 2022. Guidelines for drinking-water quality: fourth edition incorporating the first and second addenda. World Health Organization, Geneva. https:\/\/apps.who.int\/iris\/handle\/10665\/352532."},{"key":"e_1_3_4_15_2","unstructured":"VMM. 2022. Kwaliteit van het drinkwater 2022"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.5942\/jawwa.2016.108.0152"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2008.07.026"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2006.04.010"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1631\/jzus.A1600316"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2012.09.053"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2014.01.054"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2018.02.006"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-021-03008-3"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijheh.2011.05.009"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2020.127483"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00845-07"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2023.120858"},{"key":"e_1_3_4_28_2","unstructured":"EPA. 2010. A handbook on the implementation of the regulations for water service authorities for public water supplies. Johnstown Castle Ireland"},{"key":"e_1_3_4_29_2","unstructured":"European Union. 2020. Directive 2020\/2184 of the European Parliament and of the council of 16 December 2020 on the quality of water intended for human consumption (Recast)"},{"key":"e_1_3_4_30_2","unstructured":"VMM. 2021. Kwaliteit van het drinkwater 2021"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00042-24"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.3389\/frwa.2023.1022271"},{"key":"e_1_3_4_33_2","unstructured":"KWR. 2020. Coliformen in drinkwater 135"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.3390\/engproc2024069115"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibiod.2020.105032"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.658927"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2022.133827"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41545-024-00323-8"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijheh.2019.04.010"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.2166\/ws.2007.091"},{"key":"e_1_3_4_41_2","unstructured":"van den Eshof AJ Smit E Fonds AW. 1987. Resultaten van het onderzoek van drinkwater in Nederland in 1986 vergeleken met het Drinkwaterbesluit. Rijksinstituut voor Volksgezondheid en Milieu RIVM"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02867-20"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2023.113710"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6941.12145"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2012.08.043"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","unstructured":"Garc\u00eda-Timermans C Malfroot B Dierendonck C Mol Z Pluym T Waegenaar F Arends J Walgraeve C Boon N De Gusseme B. 2022. Pilot-scale drinking water distribution system to study water quality changes during transport. Res Square. doi:10.21203\/rs.3.rs-2120214\/v1","DOI":"10.21203\/rs.3.rs-2120214\/v1"},{"key":"e_1_3_4_47_2","unstructured":"Emis Vito. 2020. Compendium voor de monsterneming meting en analyse van water"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.733753"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41545-024-00371-0"},{"key":"e_1_3_4_50_2","unstructured":"International Organization for Standardization. 2014. Water quality-enumeration of Escherichia coli and coliform bacteria. ISO"},{"key":"e_1_3_4_51_2","unstructured":"R Core Team. 2022. R: a language and environment for statistical computing. R Foundation for Statistical Computing Vienna Austria. https:\/\/www.R-project.org."},{"key":"e_1_3_4_52_2","unstructured":"RStudio Team. 2020. RStudio: integrated development environment for R. RStudio PBC Boston MA. http:\/\/www.rstudio.com."},{"key":"e_1_3_4_53_2","unstructured":"EllisB HaalandP HahneF MeurML GopalakrishnanN SpidlenJ JiangM FinakF. 2022. flowCore: flowCore: basic structures for flow cytometry data"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1111\/2041-210X.12607"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3869"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1219"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061217"},{"key":"e_1_3_4_58_2","unstructured":"Oksanen J Simpson GL Blanchet FG Kindt R Legendre P Minchin PR O\u2019Hara RB Solymos P Stevens MHH Szoecs E et al.. 2022. vegan: community ecology package. https:\/\/CRAN.R-project.org\/package=vegan."},{"key":"e_1_3_4_59_2","unstructured":"Kassambara A. 2020. ggpubr: \u201cggplot2\u201d based publication ready plots. https:\/\/CRAN.R-project.org\/package=ggpubr."},{"key":"e_1_3_4_60_2","doi-asserted-by":"crossref","unstructured":"Wickham H. 2016. ggplot2: elegant graphics for data analysis. Springer-Verlag New York. https:\/\/ggplot2.tidyverse.org","DOI":"10.1007\/978-3-319-24277-4_9"},{"key":"e_1_3_4_61_2","unstructured":"Wickham H Fran\u00e7ois R Henry L M\u00fcller K. 2022. dplyr: a grammar of data manipulation. https:\/\/CRAN.R-project.org\/package=dplyr."},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022172400006987"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.3390\/w11050903"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1080\/1573062X.2020.1800759"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1061\/41203(425)47"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jenvman.2022.116510"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1080\/08927010903383448"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.2166\/wst.2013.785"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2008.09.015"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eti.2020.101032"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.54.10.2492-2499.1988"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41545-021-00139-w"},{"key":"e_1_3_4_73_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.54.3.649-654.1988"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.6b00835"},{"key":"e_1_3_4_75_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00054-07"},{"key":"e_1_3_4_76_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1551-8833.1990.tb07054.x"},{"key":"e_1_3_4_77_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1551-8833.1992.tb07305.x"},{"key":"e_1_3_4_78_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2010.07.023"},{"key":"e_1_3_4_79_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.8b02607"},{"key":"e_1_3_4_80_2","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00183-16"},{"key":"e_1_3_4_81_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00579-11"},{"key":"e_1_3_4_82_2","first-page":"1685","article-title":"The interaction between heterotrophic bacteria and coliform, fecal coliform, fecal streptococci bacteria in the water supply networks","volume":"44","author":"Amanidaz N","year":"2015","unstructured":"Amanidaz N, Zafarzadeh A, Mahvi AH. 2015. The interaction between heterotrophic bacteria and coliform, fecal coliform, fecal streptococci bacteria in the water supply networks. Iran J Public Health 44:1685\u20131692.","journal-title":"Iran J Public Health"},{"key":"e_1_3_4_83_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.7b01014"},{"key":"e_1_3_4_84_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02916-06"},{"key":"e_1_3_4_85_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijheh.2010.05.003"},{"key":"e_1_3_4_86_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1752-1688.2005.tb03793.x"},{"key":"e_1_3_4_87_2","doi-asserted-by":"publisher","DOI":"10.2166\/wst.1997.0742"},{"key":"e_1_3_4_88_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2017.08.002"},{"key":"e_1_3_4_89_2","doi-asserted-by":"publisher","DOI":"10.1061\/(ASCE)0733-9372(2009)135:5(372)"},{"key":"e_1_3_4_90_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-142-1-155"},{"key":"e_1_3_4_91_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201200503"},{"key":"e_1_3_4_92_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2020.02.015"},{"key":"e_1_3_4_93_2","doi-asserted-by":"publisher","DOI":"10.1111\/jam.12516"},{"key":"e_1_3_4_94_2","doi-asserted-by":"publisher","DOI":"10.1021\/es5009467"},{"key":"e_1_3_4_95_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2020.116261"},{"key":"e_1_3_4_96_2","doi-asserted-by":"publisher","DOI":"10.1177\/1178622119882335"},{"key":"e_1_3_4_97_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-024-02353-8"},{"key":"e_1_3_4_98_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.7.2201-2211.1996"},{"key":"e_1_3_4_99_2","doi-asserted-by":"publisher","DOI":"10.1039\/C6EW00030D"},{"key":"e_1_3_4_100_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02446"},{"key":"e_1_3_4_101_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2022.113638"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.00686-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.00686-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T09:02:12Z","timestamp":1750237332000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.00686-25"}},"subtitle":[],"editor":[{"given":"Charles M.","family":"Dozois","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,6,18]]},"references-count":100,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6,18]]}},"alternative-id":["10.1128\/aem.00686-25"],"URL":"https:\/\/doi.org\/10.1128\/aem.00686-25","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.02.19.639043","asserted-by":"object"}]},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,18]]},"assertion":[{"value":"2025-04-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00686-25"}}