{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T12:45:25Z","timestamp":1781527525242,"version":"3.54.1"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T00:00:00Z","timestamp":1721779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T00:00:00Z","timestamp":1721779200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Department of Education and Training | Australian Research Council","doi-asserted-by":"publisher","award":["DE210101063, Discovery Early Career Researcher Award"],"award-info":[{"award-number":["DE210101063, Discovery Early Career Researcher Award"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2024,7,24]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Mycoplasma bovis<\/jats:italic>\n              is an important emerging pathogen of cattle and bison, but our understanding of the genetic basis of its interactions with its host is limited. The aim of this study was to identify genes of\n              <jats:italic>M. bovis<\/jats:italic>\n              required for interaction and survival in association with host cells. One hundred transposon-induced mutants of the type strain PG45 were assessed for their capacity to survive and proliferate in Madin-Darby bovine kidney cell cultures. The growth of 19 mutants was completely abrogated, and 47 mutants had a prolonged doubling time compared to the parent strain. All these mutants had a similar growth pattern to the parent strain PG45 in the axenic media. Thirteen genes previously classified as dispensable for the axenic growth of\n              <jats:italic>M. bovis<\/jats:italic>\n              were found to be essential for the growth of\n              <jats:italic>M. bovis<\/jats:italic>\n              in association with host cells. In most of the mutants with a growth-deficient phenotype, the transposon was inserted into a gene involved in transportation or metabolism. This included genes coding for ABC transporters, proteins related to carbohydrate, nucleotide and protein metabolism, and membrane proteins essential for attachment. It is likely that these genes are essential not only\n              <jats:italic>in vitro<\/jats:italic>\n              but also for the survival of\n              <jats:italic>M. bovis<\/jats:italic>\n              in infected animals.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              <jats:italic>Mycoplasma bovis<\/jats:italic>\n              causes chronic bronchopneumonia, mastitis, arthritis, keratoconjunctivitis, and reproductive tract disease in cattle around the globe and is an emerging pathogen in bison. Control of mycoplasma infections is difficult in the absence of appropriate antimicrobial treatment or effective vaccines. A comprehensive understanding of host-pathogen interactions and virulence factors is important to implement more effective control methods against\n              <jats:italic>M. bovis<\/jats:italic>\n              . Recent studies of other mycoplasmas with\n              <jats:italic>in vitro<\/jats:italic>\n              cell culture models have identified essential virulence genes of mycoplasmas. Our study has identified genes of\n              <jats:italic>M. bovis<\/jats:italic>\n              required for survival in association with host cells, which will pave the way to a better understanding of host-pathogen interactions and the role of specific genes in the pathogenesis of disease caused by\n              <jats:italic>M. bovis<\/jats:italic>\n              .\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aem.00687-24","type":"journal-article","created":{"date-parts":[[2024,6,12]],"date-time":"2024-06-12T13:06:51Z","timestamp":1718197611000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Genes required for survival and proliferation of\n            <i>Mycoplasma bovis<\/i>\n            in association with host cells"],"prefix":"10.1128","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1342-3896","authenticated-orcid":false,"given":"Chintha K.","family":"Premachandre","sequence":"first","affiliation":[{"name":"Asia-Pacific Center for Animal Health, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6877-7383","authenticated-orcid":true,"given":"Paola K.","family":"Vaz","sequence":"additional","affiliation":[{"name":"Asia-Pacific Center for Animal Health, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shukriti","family":"Sharma","sequence":"additional","affiliation":[{"name":"Asia-Pacific Center for Animal Health, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Kanci Condello","sequence":"additional","affiliation":[{"name":"Asia-Pacific Center for Animal Health, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0903-2469","authenticated-orcid":true,"given":"Glenn F.","family":"Browning","sequence":"additional","affiliation":[{"name":"Asia-Pacific Center for Animal Health, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4426-5011","authenticated-orcid":true,"given":"Nadeeka K.","family":"Wawegama","sequence":"additional","affiliation":[{"name":"Asia-Pacific Center for Animal Health, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2007.09.007"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvfa.2020.02.002"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12860"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8010124"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0087672"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-014-0256-x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1071\/AN16490"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2015.02.024"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-142-9-2463"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.2478\/bvip-2013-0087"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01195-09"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025291"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008661"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0097100"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8020164"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.2357210"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00154-20"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00233"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-003-4159-5"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10071366"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00055-17"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2019.05.008"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2018.08.001"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.107722"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1177263"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0076-6879(98)92006-7"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0923-2508(01)01193-7"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1590\/S1415-47572007000200006"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03112-20"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00154-10"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.067553-0"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.8.1.85-92.2001"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-016-0387-0"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1080\/03079457.2019.1637514"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-55"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-11-185"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01212-12"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.19.6824-6831.2005"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00403-15"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00030.2002"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2021.644224"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1600546113"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.19.5645-5650.2001"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036247"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0378-1135(02)00303-6"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00329"},{"key":"e_1_3_3_48_2","first-page":"232","volume-title":"Theory and practice in experimental bacteriology","author":"Meynell GG","year":"1970","unstructured":"Meynell GG, Meynell E. 1970. Theory and practice in experimental bacteriology. 2nd ed, p 232\u2013233. Cambridge University Press, Cambridge.","edition":"2"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-017-0499-1"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00726-10"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1100"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2015.053"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.1.29"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbx085"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.00687-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.00687-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,20]],"date-time":"2024-08-20T17:23:23Z","timestamp":1724174603000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.00687-24"}},"subtitle":[],"editor":[{"given":"Charles M.","family":"Dozois","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,7,24]]},"references-count":53,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7,24]]}},"alternative-id":["10.1128\/aem.00687-24"],"URL":"https:\/\/doi.org\/10.1128\/aem.00687-24","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,24]]},"assertion":[{"value":"2024-04-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00687-24"}}