{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:12:59Z","timestamp":1791288779061,"version":"4.3.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Antibiotic-resistant bacteria (ARB) have been surveyed widely in water bodies, but few studies have determined the diversity of ARB in sediment, which is the most taxon-abundant habitat in aquatic environments. We isolated 56 extended-spectrum \u03b2-lactamase (ESBL)-producing bacteria from a single sediment sample taken from an urban river in China. All strains were confirmed for ESBL-producing capability by both the clavulanic acid combination disc method and MIC determination. Of the isolated strains, 39 were classified as<jats:italic>Enterobacteriaceae<\/jats:italic>(consisting of the genera<jats:italic>Escherichia<\/jats:italic>,<jats:italic>Klebsiella<\/jats:italic>,<jats:italic>Serratia<\/jats:italic>, and<jats:italic>Aeromonas<\/jats:italic>) by 16S rRNA gene sequencing and biochemical analysis. The present study identifies, for the first time, ESBL-producing strains from the families<jats:italic>Brucellaceae<\/jats:italic>and<jats:italic>Moraxellaceae<\/jats:italic>. The<jats:italic>bla<\/jats:italic><jats:sub>CTX-M<\/jats:sub>gene was the most dominant of the ESBL genes (45 strains), while the<jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub>gene was the second-most dominant (22 strains). A total of five types of<jats:italic>bla<\/jats:italic><jats:sub>CTX-M<\/jats:sub>fragments were identified, with both known and novel sequences. A library of<jats:italic>bla<\/jats:italic><jats:sub>CTX-M<\/jats:sub>cloned from the sediment DNA showed an even higher diversity of<jats:italic>bla<\/jats:italic><jats:sub>CTX-M<\/jats:sub>sequences. The discovery of highly diverse ESBL-producing bacteria and ESBL genes, particularly<jats:italic>bla<\/jats:italic><jats:sub>CTX<\/jats:sub>, in urban river sediment raises alarms for potential dissemination of ARB in communities through river environments.<\/jats:p>","DOI":"10.1128\/aem.00711-10","type":"journal-article","created":{"date-parts":[[2010,7,17]],"date-time":"2010-07-17T02:01:16Z","timestamp":1279332076000},"page":"5972-5976","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":86,"title":["High Diversity of Extended-Spectrum Beta-Lactamase-Producing Bacteria in an Urban River Sediment Habitat"],"prefix":"10.1128","volume":"76","author":[{"given":"Su-Ying","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China 510515"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Nan Fang Hospital, Southern Medical University, Guangzhou, China 510515"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sui-Na","family":"Geng","sequence":"additional","affiliation":[{"name":"Nan Fang Hospital, Southern Medical University, Guangzhou, China 510515"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian-Yu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China 510515"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuo-Ming","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China 510515"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Sheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Medical Oncology Department, Cancer Center, Sun Yat-sen University, Guangzhou, China 510060"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China 510515"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Wei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China 510515"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2008.05.006"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"423","DOI":"10.3201\/eid1403.070405","volume":"14","year":"2008","unstructured":"Barlow, M., R. 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