{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:17:12Z","timestamp":1787242632211,"version":"build-2736575974"},"reference-count":83,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2025,9,17]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Vibrio spp.<\/jats:italic>\n                      comprise ecologically significant bacteria that thrive in warm, moderately saline water, and their incidence and proliferation are strongly influenced by environmental factors. In recent years,\n                      <jats:italic toggle=\"yes\">Vibrio spp<\/jats:italic>\n                      . infections have been reported more frequently and over a greater geographical area along the US eastern seaboard. This study provides an analysis of\n                      <jats:italic toggle=\"yes\">Vibrio spp<\/jats:italic>\n                      . infections, notably caused by\n                      <jats:italic toggle=\"yes\">Vibrio alginolyticus<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Vibrio cholerae<\/jats:italic>\n                      non-O1\/non-O139,\n                      <jats:italic toggle=\"yes\">Vibrio fluvialis<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Vibrio mimicus<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">V. parahaemolyticus<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">V. vulnificus<\/jats:italic>\n                      , extracted from the Centers for Disease Control and Prevention\u2019s Cholera and Other\n                      <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                      Illness Surveillance system, located within 200 km of the eastern US coast, to analyze latitudinal distribution trends between 1990 and 2019. For each\n                      <jats:italic toggle=\"yes\">Vibrio spp<\/jats:italic>\n                      ., case incidence (presence\/absence) was modeled, including environmental data and employing extreme gradient boosting machine learning algorithms (XGBoost). Environmental parameters associated with the incidence of vibriosis were clustered using k-means clustering. The northern limit of total cases of vibriosis was found to have increased\n                      <jats:italic toggle=\"yes\">ca<\/jats:italic>\n                      . 40 km\/year, with\n                      <jats:italic toggle=\"yes\">V. alginolyticus<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">ca<\/jats:italic>\n                      . 70 km\/year),\n                      <jats:italic toggle=\"yes\">V. fluvialis<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">ca<\/jats:italic>\n                      . 60 km\/year), and\n                      <jats:italic toggle=\"yes\">Vibrio parahaemolyticus<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">ca<\/jats:italic>\n                      . 60 km\/year) showing the greatest latitudinal shifts. These changes were found to be linked to environmental parameters that enhance the proliferation of\n                      <jats:italic toggle=\"yes\">Vibrio spp<\/jats:italic>\n                      . The average accuracy of the XGBoost models was 60.9%\u201371.0%, with temperature and salinity being the most significant predictors. Relationships among other environmental parameters were complex and nonlinear, but phytoplankton and precipitation served to differentiate the models. Clustering using k-means yielded results that supported temperature, salinity, and phytoplankton as important environmental parameters. Research in progress will aid in developing global predictive risk models for\n                      <jats:italic toggle=\"yes\">Vibrio spp<\/jats:italic>\n                      . infections.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Vibrio spp<\/jats:italic>\n                        . are ecologically significant bacteria, and their incidence and proliferation are strongly influenced by environmental factors. In recent years,\n                        <jats:italic toggle=\"yes\">Vibrio spp<\/jats:italic>\n                        . infections have been reported more frequently and over a greater geographical area along the US eastern seaboard. This study provides an analysis of latitudinal distribution trends of\n                        <jats:italic toggle=\"yes\">Vibrio spp<\/jats:italic>\n                        . infections, notably caused by\n                        <jats:italic toggle=\"yes\">Vibrio alginolyticus<\/jats:italic>\n                        ,\n                        <jats:italic toggle=\"yes\">Vibrio cholerae<\/jats:italic>\n                        non-O1\/non-O139,\n                        <jats:italic toggle=\"yes\">Vibrio fluvialis<\/jats:italic>\n                        ,\n                        <jats:italic toggle=\"yes\">Vibrio mimicus<\/jats:italic>\n                        ,\n                        <jats:italic toggle=\"yes\">Vibrio parahaemolyticus<\/jats:italic>\n                        , and\n                        <jats:italic toggle=\"yes\">V. vulnificus<\/jats:italic>\n                        , within 200 km of the eastern US coast. The northern limit of total cases of vibriosis was found to have increased\n                        <jats:italic toggle=\"yes\">ca<\/jats:italic>\n                        . 40 km\/year. These changes were found to be linked to environmental parameters that enhance the proliferation of\n                        <jats:italic toggle=\"yes\">Vibrio spp<\/jats:italic>\n                        . Temperature and salinity were the most significant predictors of vibriosis case presence and absence. Phytoplankton and precipitation changes served to differentiate\n                        <jats:italic toggle=\"yes\">Vibrio sp<\/jats:italic>\n                        . presence. Research in progress will aid in developing global predictive risk models for\n                        <jats:italic toggle=\"yes\">Vibrio spp<\/jats:italic>\n                        . infections.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/aem.00716-25","type":"journal-article","created":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T18:30:26Z","timestamp":1755196226000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Machine learning in understanding environmental variability of vibriosis in coastal waters"],"prefix":"10.1128","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9953-6651","authenticated-orcid":false,"given":"Bailey M.","family":"Magers","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02y3ad647","id-type":"ROR","asserted-by":"publisher"}],"name":"Geohealth and Hydrology Laboratory, Department of Environmental Engineering Sciences, University of Florida","place":["Gainesville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3234-3337","authenticated-orcid":true,"given":"Kyle D.","family":"Brumfield","sequence":"additional","affiliation":[{"name":"Maryland Pathogen Research Institute, University of Maryland","place":["College Park, USA"]},{"name":"University of Maryland Institute for Advanced Computer Studies, University of Maryland","place":["College Park, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunil","family":"Kumar","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02y3ad647","id-type":"ROR","asserted-by":"publisher"}],"name":"Geohealth and Hydrology Laboratory, Department of Environmental Engineering Sciences, University of Florida","place":["Gainesville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5432-1502","authenticated-orcid":true,"given":"Rita R.","family":"Colwell","sequence":"additional","affiliation":[{"name":"Maryland Pathogen Research Institute, University of Maryland","place":["College Park, USA"]},{"name":"University of Maryland Institute for Advanced Computer Studies, University of Maryland","place":["College Park, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8191-2348","authenticated-orcid":true,"given":"Antarpreet S.","family":"Jutla","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02y3ad647","id-type":"ROR","asserted-by":"publisher"}],"name":"Geohealth and Hydrology Laboratory, Department of Environmental Engineering Sciences, University of Florida","place":["Gainesville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-6735-9_12"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15716"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.6.1977-1980.1990"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.marpolbul.2017.07.054"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01035-17"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00282"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.marpolbul.2018.05.007"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.6.2592-2597.1999"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.7.4103-4110.2004"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11430-017-9229-x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2017.01.049"},{"key":"e_1_3_3_13_2","unstructured":"CDC. 2024. Vibrio Infection (Vibriosis). Available from: https:\/\/www.cdc.gov\/vibrio\/about\/index.html"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00307-23"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-28247-2"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1012767"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis243"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2023.117940"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.14967"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1609157113"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2011.11-0181"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.4.1438"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15040"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2016.09.008"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2008.78.823"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envsoft.2013.05.008"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0809654105"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01147-17"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12237-013-9682-4"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","unstructured":"Johnson CN Bowers JC Griffitt KJ Molina V Clostio RW Pei S et al.. 2012 Ecology of Vibrio parahaemolyticus and Vibrio vulnificus in the coastal and estuarine waters of Louisiana 78:7249\u20137257. doi:10.1128\/AEM.01296-12","DOI":"10.1128\/AEM.01296-12"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0185994"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5295.2025"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.45.1.275-283.1983"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.2307\/3225146"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.5194\/bg-7-621-2010"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004153"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0137828"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.epidem.2013.09.004"},{"key":"e_1_3_3_39_2","unstructured":"CDC. 2005. Vibrio illnesses after hurricane katrina --- multiple states August--September 2005. Available from: https:\/\/www.cdc.gov\/mmWr\/preview\/mmwrhtml\/mm5437a5.htm"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01848-15"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-70816-2"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2022.959830"},{"key":"e_1_3_3_43_2","first-page":"494","volume-title":"Bergey\u2019s Manual of Systematic Bacteriology","author":"Farmer JJ","year":"2005","unstructured":"Farmer JJ, Janda JM, Brenner FW, Cameron DN, Birkhead KM. 2005. Genus Vibrio, p 494\u2013546. In Krieg NR, Staley JT (ed), Bergey\u2019s Manual of Systematic Bacteriology, 2nd edn. Springer, New York.","edition":"2"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555815714.ch12"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2007.00303.x"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.12-0721"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1097\/EDE.0b013e31815c09ea"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.17-0048"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1061\/(ASCE)WR.1943-5452.0000929"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-22946-y"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1029\/2022GH000681"},{"key":"e_1_3_3_52_2","unstructured":"FDA. 2005. Pathogenic Vibrio parahaemolyticus in raw oysters"},{"key":"e_1_3_3_53_2","unstructured":"National Centers for Coastal Ocean Science. 2018. Vibrio predictive models. Available from: https:\/\/products.coastalscience.noaa.gov\/vibrioforecast\/"},{"key":"e_1_3_3_54_2","unstructured":"National Centers for Coastal Ocean Science. 2019. Water guidance - Vibrio vulnificus in Chesapeake Bay. Available from: https:\/\/products.coastalscience.noaa.gov\/vibrioforecast\/vulnificus\/chesapeake\/default.aspx"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rse.2020.112016"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1289\/EHP2198"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1289\/EHP15593"},{"key":"e_1_3_3_58_2","unstructured":"CDC. 2024. Cholera and Other Vibrio Illness Surveillance System. Available from: https:\/\/www.cdc.gov\/vibrio\/php\/surveillance\/index.html"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1175\/JCLI-D-20-0166.1"},{"key":"e_1_3_3_60_2","unstructured":"NASA. 2024. Ocean Color Level 3&4 Browser. Available from: https:\/\/oceancolor.gsfc.nasa.gov\/"},{"key":"e_1_3_3_61_2","unstructured":"Copernicus Climate Change Service CDS. 2021. ORAS5 Global Ocean Reanalysis Monthly Data from 1958 to Present. Copernicus Climate Change Service (C3S) Climate Data Store (CDS. Available from: https:\/\/doi.org\/10.24381\/cds.67e8eeb7"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","unstructured":"Hersbach H Bell B Berrisford P Biavati G Hor\u00e1nyi A Mu\u00f1oz Sabater J. 2023. ERA5 monthly averaged data on single levels from 1940 to present. Copernicus Climate Change Service (C3S). doi:10.24381\/cds.f17050d7","DOI":"10.24381\/cds.f17050d7"},{"key":"e_1_3_3_63_2","unstructured":"E.U. Copernicus Marine Service Information (CMEMS) Marine Data Store (MDS). 2024. Global ocean colour (copernicus-globcolour) bio-geo-chemical L4 (monthly and interpolated) from satellite observations (1997-ongoing)"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.5194\/bg-8-311-2011"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1145\/2939672.2939785"},{"key":"e_1_3_3_66_2","first-page":"4768","volume-title":"Proceedings of the 31st International Conference on Neural Information Processing Systems","author":"Lundberg SM","year":"2017","unstructured":"Lundberg SM, Lee SI. 2017. A unified approach to interpreting model predictions. Proceedings of the 31st International Conference on Neural Information Processing Systems. , p 4768\u20134777Curran Associates Inc, Red Hook, NY, USA"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1175\/2009JPO4300.1"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-34934-x"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1175\/JCLI-D-20-0366.1"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.3354\/cr00953"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1029\/2004GL021808"},{"key":"e_1_3_3_72_2","unstructured":"NOAA. 2025. NOAA Office for Coastal Management. Available from: https:\/\/coast.noaa.gov\/states\/fast-facts\/economics-and-demographics.html#:~:text=California%20tops%20the%20coastal%20populations and%20Texas%20with%206.9%20million"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.12.3150-3155.1997"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2022.27.28.2101088"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2010.04.001"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/2716789"},{"key":"e_1_3_3_77_2","unstructured":"Calvin K Dasgupta D Krinner G Mukherji A Thorne PW Trisos C. 2023. Climate change 2023: synthesis report. contribution of working groups I II and III to the sixth assessment report of the intergovernmental panel on climate change. Edited by H. Lee and J. Romero. Geneva Switzerland IPCC"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.44.5.1047-1058.1982"},{"key":"e_1_3_3_79_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02680-23"},{"key":"e_1_3_3_80_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.55.8.2073-2078.1989"},{"key":"e_1_3_3_81_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15041"},{"key":"e_1_3_3_82_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01698-08"},{"key":"e_1_3_3_83_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.02136.x"},{"key":"e_1_3_3_84_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.marenvres.2022.105828"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.00716-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.00716-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T21:44:10Z","timestamp":1758145450000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.00716-25"}},"subtitle":[],"editor":[{"given":"Julia C.","family":"van Kessel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,9,17]]},"references-count":83,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9,17]]}},"alternative-id":["10.1128\/aem.00716-25"],"URL":"https:\/\/doi.org\/10.1128\/aem.00716-25","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,17]]},"assertion":[{"value":"2025-04-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00716-25"}}