{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:56:12Z","timestamp":1789001772040,"version":"build-2803163510"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2016,8,15]],"date-time":"2016-08-15T00:00:00Z","timestamp":1471219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The temperature-dependent alteration of flagellar motility gene expression is critical for the foodborne pathogen\n            <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n            to respond to a changing environment. In this study, a genetic determinant,\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            <jats:italic>f2365_0220<\/jats:italic>\n            (\n            <jats:italic>lmof2365_0220<\/jats:italic>\n            ), encoding a putative protein that is structurally similar to the\n            <jats:named-content content-type=\"genus-species\">Bacillus cereus<\/jats:named-content>\n            alkyl base DNA glycosylase (AlkD), was identified. This determinant was involved in the transcriptional repression of flagellar motility genes and was named\n            <jats:italic>adlP<\/jats:italic>\n            (encoding an\n            <jats:underline>A<\/jats:underline>\n            lk\n            <jats:underline>D<\/jats:underline>\n            -\n            <jats:underline>l<\/jats:underline>\n            ike\n            <jats:underline>p<\/jats:underline>\n            rotein [AdlP]). Deletion of\n            <jats:italic>adlP<\/jats:italic>\n            activated the expression of flagellar motility genes at 37\u00b0C and disrupted the temperature-dependent inhibition of\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            motility. The\n            <jats:italic>adlP<\/jats:italic>\n            null strains demonstrated decreased survival in murine macrophage-like RAW264.7 cells and less virulence in mice. Furthermore, the deletion of\n            <jats:italic>adlP<\/jats:italic>\n            significantly decreased biofilm formation and impaired the survival of bacteria under several stress conditions, including the presence of a DNA alkylation compound (methyl methanesulfonate), an oxidative agent (H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            ), and aminoglycoside antibiotics. Our findings strongly suggest that\n            <jats:italic>adlP<\/jats:italic>\n            may encode a bifunctional protein that transcriptionally represses the expression of flagellar motility genes and influences stress responses through its DNA glycosylase activity.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            We discovered a novel protein that we named\n            <jats:underline>A<\/jats:underline>\n            lk\n            <jats:underline>D<\/jats:underline>\n            -\n            <jats:underline>l<\/jats:underline>\n            ike\n            <jats:underline>p<\/jats:underline>\n            rotein (AdlP). This protein affected flagellar motility, biofilm formation, and virulence. Our data suggest that AdlP may be a bifunctional protein that represses flagellar motility genes and influences stress responses through its DNA glycosylase activity.\n          <\/jats:p>","DOI":"10.1128\/aem.00719-16","type":"journal-article","created":{"date-parts":[[2016,6,18]],"date-time":"2016-06-18T02:23:51Z","timestamp":1466216631000},"page":"5144-5152","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Listeria monocytogenes DNA Glycosylase AdlP Affects Flagellar Motility, Biofilm Formation, Virulence, and Stress Responses"],"prefix":"10.1128","volume":"82","author":[{"given":"Ting","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Starkville, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongryeoul","family":"Bae","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Starkville, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chinling","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Starkville, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1413"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.3.584-640.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08080"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.01.005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-20"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-13-156"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01281-07"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01085-07"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002887"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.5.3152-3159.2005"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06589.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04776.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05328.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-10-641"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.08.046"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.4161\/rna.19235"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1112371108"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01751.x"},{"key":"e_1_3_2_20_2","first-page":"2171","article-title":"Temperature-dependent expression of flagella of Listeria monocytogenes studied by electron microscopy, SDS-PAGE and Western blotting","volume":"134","author":"Peel M","year":"1988","unstructured":"Peel M, Donachie W, Shaw A. 1988. 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