{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:20:09Z","timestamp":1790317209919,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2009,9,15]],"date-time":"2009-09-15T00:00:00Z","timestamp":1252972800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2009,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In an attempt to understand the microorganisms involved in the generation of\n            <jats:italic>trans<\/jats:italic>\n            -1,2-dichloroethene (\n            <jats:italic>trans<\/jats:italic>\n            -DCE), pure-culture \u201c\n            <jats:italic>Dehalococcoides<\/jats:italic>\n            \u201d sp. strain MB was isolated from environmental sediments. In contrast to currently known tetrachloroethene (PCE)- or trichloroethene (TCE)-dechlorinating pure cultures, which generate\n            <jats:italic>cis<\/jats:italic>\n            -DCE as the predominant product,\n            <jats:italic>Dehalococcoides<\/jats:italic>\n            sp. strain MB reductively dechlorinates PCE to\n            <jats:italic>trans<\/jats:italic>\n            -DCE and\n            <jats:italic>cis<\/jats:italic>\n            -DCE at a ratio of 7.3 (\u00b10.4):1. It utilizes H\n            <jats:sub>2<\/jats:sub>\n            as the sole electron donor and PCE or TCE as the electron acceptor during anaerobic respiration. Strain MB is a disc-shaped, nonmotile bacterium. Under an atomic force microscope, the cells appear singly or in pairs and are 1.0 \u03bcm in diameter and \u223c150 nm in depth. The purity was confirmed by culture-based approaches and 16S rRNA gene-based analysis and was corroborated further by putative reductive dehalogenase (RDase) gene-based, quantitative real-time PCR. Although strain MB shares 100% 16S rRNA gene sequence identity with\n            <jats:italic>Dehalococcoides ethenogenes<\/jats:italic>\n            strain 195, these two strains possess different dechlorinating pathways. Microarray analysis revealed that 10 putative RDase genes present in strain 195 were also detected in strain MB. Successful cultivation of strain MB indicates that the biotic process could contribute significantly to the generation of\n            <jats:italic>trans<\/jats:italic>\n            -DCE in chloroethene-contaminated sites. It also enhances our understanding of the evolution of this unusual microbial group,\n            <jats:italic>Dehalococcoides<\/jats:italic>\n            species.\n          <\/jats:p>","DOI":"10.1128\/aem.00767-09","type":"journal-article","created":{"date-parts":[[2009,7,25]],"date-time":"2009-07-25T00:59:42Z","timestamp":1248483582000},"page":"5910-5918","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":103,"title":["Isolation and Characterization of \u201c\n            <i>Dehalococcoides<\/i>\n            \u201d sp. Strain MB, Which Dechlorinates Tetrachloroethene to\n            <i>trans<\/i>\n            -1,2-Dichloroethene"],"prefix":"10.1128","volume":"75","author":[{"given":"Dan","family":"Cheng","sequence":"first","affiliation":[{"name":"Division of Environmental Science and Engineering, National University of Singapore, Singapore 117576, Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhong","family":"He","sequence":"additional","affiliation":[{"name":"Division of Environmental Science and Engineering, National University of Singapore, Singapore 117576, Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01649-07"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/35046063"},{"key":"e_1_3_2_4_2","unstructured":"Statistical algorithms reference guide. 2001"},{"key":"e_1_3_2_5_2","unstructured":"Agency for 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