{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T04:22:45Z","timestamp":1784175765883,"version":"3.55.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2012,7,15]],"date-time":"2012-07-15T00:00:00Z","timestamp":1342310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Bananas are among the most widely consumed foods in the world. In Uganda, the country with the second largest banana production in the world, bananas are the most important staple food. The objective of this study was to analyze banana-associated microorganisms and to select efficient antagonists against fungal pathogens which are responsible for substantial yield losses. We studied the structure and function of microbial communities (endosphere, rhizosphere, and soil) obtained from three different traditional farms in Uganda by cultivation-independent (PCR-SSCP fingerprints of 16S rRNA\/ITS genes, pyrosequencing of enterobacterial 16S rRNA gene fragments, quantitative PCR, fluorescence\n            <jats:italic>in situ<\/jats:italic>\n            hybridization coupled with confocal laser scanning microscopy, and PCR-based detection of broad-host-range plasmids and sulfonamide resistance genes) and cultivation-dependent methods. The results showed microhabitat-specific microbial communities that were significant across sites and treatments. Furthermore, all microhabitats contained a high number and broad spectrum of indigenous antagonists toward identified fungal pathogens. While bacterial antagonists were found to be enriched in banana plants, fungal antagonists were less abundant and mainly found in soil. The banana stem endosphere was the habitat with the highest bacterial counts (up to 10\n            <jats:sup>9<\/jats:sup>\n            gene copy numbers g\n            <jats:sup>\u22121<\/jats:sup>\n            ). Here, enterics were found to be enhanced in abundance and diversity; they provided one-third of the bacteria and were identified by pyrosequencing with 14 genera, including not only potential human (\n            <jats:named-content content-type=\"genus-species\">Escherichia<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Klebsiella<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Yersinia<\/jats:named-content>\n            spp.) and plant (\n            <jats:named-content content-type=\"genus-species\">Pectobacterium<\/jats:named-content>\n            spp.) pathogens but also disease-suppressive bacteria (\n            <jats:named-content content-type=\"genus-species\">Serratia<\/jats:named-content>\n            spp.). The dominant role of enterics can be explained by the permanent nature and vegetative propagation of banana and the amendments of human, as well as animal, manure in these traditional cultivations.\n          <\/jats:p>","DOI":"10.1128\/aem.00772-12","type":"journal-article","created":{"date-parts":[[2012,5,5]],"date-time":"2012-05-05T09:51:36Z","timestamp":1336211496000},"page":"4933-4941","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["Banana-Associated Microbial Communities in Uganda Are Highly Diverse but Dominated by Enterobacteriaceae"],"prefix":"10.1128","volume":"78","author":[{"given":"Bettina","family":"Rossmann","sequence":"first","affiliation":[{"name":"Graz University of Technology, Institute of Environmental Biotechnology, Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Graz University of Technology, Institute of Environmental Biotechnology, Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kornelia","family":"Smalla","sequence":"additional","affiliation":[{"name":"Julius K\u00fchn-Institut, Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Mpiira","sequence":"additional","affiliation":[{"name":"Kawanda Agricultural Research Institute, National Banana Research Program, Kampala, Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Baptist","family":"Tumuhairwe","sequence":"additional","affiliation":[{"name":"Makerere University, Department of Agricultural Production, College of Agricultural and Environmental Sciences, Kampala, Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Staver","sequence":"additional","affiliation":[{"name":"Biodiversity International, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Berg","sequence":"additional","affiliation":[{"name":"Graz University of Technology, Institute of Environmental Biotechnology, Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"3389","volume-title":"Nucleic Acids Res.","author":"Altschul SF","year":"1997","unstructured":"AltschulSF . 1997. Gapped BLAST and PSI-BLAST: a new generation of protein data-base search programs. Nucleic Acids Res. 25: 3389\u2013 3402."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.56.6.1919-1925.1990"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2009.05.004"},{"key":"e_1_3_3_5_2","first-page":"157","article-title":"Awareness of banana bacterial wilt control in Uganda. 1. Farmers' perspective","volume":"14","author":"Bagamba F","year":"2006","unstructured":"BagambaF . 2006. Awareness of banana bacterial wilt control in Uganda. 1. Farmers' perspective. Afr. Crop Sci. J. 14: 157\u2013 164.","journal-title":"Afr. Crop Sci. J."},{"key":"e_1_3_3_6_2","first-page":"81","article-title":"Fast and sensitive silver staining of DNA in polyacrylamide gels","volume":"80","author":"Bassam BJ","year":"1991","unstructured":"BassamBJ Caetano-AnollesG GresshoffPM . 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal. Biochem. 80: 81\u2013 84.","journal-title":"Anal. Biochem."},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.7.3328-3338.2002"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2005.00891.x"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2005.00025.x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-009-2092-7"},{"key":"e_1_3_3_11_2","unstructured":"BinhCTT\nHeuerH\nGomesNCM\nKaupenjohannM\nSmallaK\n. \n2010. \nSimilar bacterial community structure and high abundances of sulfonamide resistance genes in field-scale manures p \n141\u2013\n166. \nNova Science Publishers Hauppauge NY."},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000197"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.44.070505.143359"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.f.303"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(99)80053-8"},{"key":"e_1_3_3_16_2","first-page":"33","article-title":"Agroecological distribution of banana systems in the Great Lakes Region","volume":"12","author":"Eledu CA","year":"2010","unstructured":"EleduCA KaramuraEB TushemereirweWK . 2010. Agroecological distribution of banana systems in the Great Lakes Region. Afr. Crop Sci. J. 12: 33\u2013 42.","journal-title":"Afr. Crop Sci. J."},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10947"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycres.2006.03.008"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2011.01.012"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0014479702000145"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.7.2621-2628.1996"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1129"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2005.05.009"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.8.3233-3241.1997"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00002"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkf164"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2004.01.002"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.4.1875-1883.2003"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl856"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.62.081307.162918"},{"key":"e_1_3_3_31_2","first-page":"4504","article-title":"Phylogenetic oligodeoxynucleotide probes for the major subclasses of Proteobacteria: problems and solutions","volume":"62","author":"Manz W","year":"1992","unstructured":"ManzW AmannR LudwigW WagnerM SchleiferK-H . 1992. Phylogenetic oligodeoxynucleotide probes for the major subclasses of Proteobacteria: problems and solutions. Syst. Appl. Microbiol. 62: 4504\u2013 4513.","journal-title":"Syst. Appl. Microbiol."},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.2174\/187220810790069523"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1203980"},{"key":"e_1_3_3_34_2","first-page":"177","article-title":"Survival and activity of the Ralstonia solanacearum antagonist Pseudomonas chlororaphis 24-4 in the rhizosphere of tomato and its impact on the indigenous bacterial community","volume":"27","author":"Milling A","year":"2004","unstructured":"MillingA LembkeA Sch\u00f6nfeldJ SmallaK . 2004. Survival and activity of the Ralstonia solanacearum antagonist Pseudomonas chlororaphis 24-4 in the rhizosphere of tomato and its impact on the indigenous bacterial community. IOBC\/WPRS Bull. 27: 177\u2013 186.","journal-title":"IOBC\/WPRS Bull."},{"key":"e_1_3_3_35_2","first-page":"1","article-title":"Untersuchungen \u00fcber die morphologische und biologische Differenzierung in der Fusarium-Sektion Liseola","volume":"169","author":"Nirenberg HI","year":"1976","unstructured":"NirenbergHI . 1976. Untersuchungen \u00fcber die morphologische und biologische Differenzierung in der Fusarium-Sektion Liseola. Mitt. Biol. Bundesanst. Land-Forstwirtsch. Berl.-Dahl. 169: 1\u2013 117.","journal-title":"Mitt. Biol. Bundesanst. Land-Forstwirtsch. Berl.-Dahl."},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2002.01668.x"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-007-9301-7"},{"key":"e_1_3_3_38_2","first-page":"151","volume-title":"DNA markers: protocols, applications, and overviews","author":"Rademaker JLW","year":"1997","unstructured":"RademakerJLW De BruijnFJ . 1997. Characterization and classification of microbes by REP-PCR genomic fingerprinting and computer-assisted pattern analysis, p 151\u2013 171. In Caetano-AnolleG GresshoffPM (ed), DNA markers: protocols, applications, and overviews. Wiley, New York, NY."},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2011.04.004"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1079\/9781845936587.0000"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf103835z"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.12.4870-4876.1998"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-80-880"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2010.11.007"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.4314\/acsj.v12i1.27658"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990140205"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-97-2-0250"},{"key":"e_1_3_3_48_2","doi-asserted-by":"crossref","unstructured":"WhiteTJ\nBrunsTD\nLeeSB\nTaylorJW\n. \n1990. \nPCR protocols: a guide to methods and applications: amplification and direct sequencing of fungal rRNA genes for phylogenetics p \n315\u2013\n322. \nAcademic Press New York NY.","DOI":"10.1016\/B978-0-12-372180-8.50042-1"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0147-52-47"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-007-9257-7"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2010.00930.x"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00772-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00772-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T01:24:07Z","timestamp":1645579447000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.00772-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7,15]]},"references-count":50,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2012,7,15]]}},"alternative-id":["10.1128\/AEM.00772-12"],"URL":"https:\/\/doi.org\/10.1128\/aem.00772-12","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7,15]]},"assertion":[{"value":"2012-03-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-04-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-06-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}