{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T13:51:34Z","timestamp":1760709094231},"reference-count":39,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The Gram-positive bacterium\n            <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n            causes a significant percentage of the fatalities among foodborne illnesses in humans. Surface proteins specifically expressed in a wide range of\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            serotypes under selective enrichment culture conditions could serve as potential biomarkers for detection and isolation of this pathogen via antibody-based methods. Our study aimed to identify such biomarkers. Interrogation of the\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            serotype 4b strain F2365 genome identified 130 putative or known surface proteins. The homologues of four surface proteins, LMOf2365_0578, LMOf2365_0581, LMOf2365_0639, and LMOf2365_2117, were assessed as biomarkers due to the presence of conserved regions among strains of\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            which are variable among other\n            <jats:named-content content-type=\"genus-species\">Listeria<\/jats:named-content>\n            species. Rabbit polyclonal antibodies against the four recombinant proteins revealed the expression of only LMOf2365_0639 on the surface of serotype 4b strain LI0521 cells despite PCR detection of mRNA transcripts for all four proteins in the organism. Three of 35 monoclonal antibodies (MAbs) to LMOf2365_0639, MAbs M3643, M3644, and M3651, specifically recognized 42 (91.3%) of 46\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            lineage I and II isolates grown in nonselective brain heart infusion medium. While M3644 and M3651 reacted with 14 to 15 (82.4 to 88.2%) of 17\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            lineage I and II isolates, M3643 reacted with 22 (91.7%) of 24 lineage I, II, and III isolates grown in selective enrichment media (UVM1, modified Fraser, Palcam, and UVM2 media). The three MAbs exhibited only weak reactivities (the optical densities at 414 nm were close to the cutoff value) to some other\n            <jats:named-content content-type=\"genus-species\">Listeria<\/jats:named-content>\n            species grown in selective enrichment media. Collectively, the data indicate the potential of LMOf2365_0639 as a surface biomarker of\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            , with the aid of specific MAbs, for pathogen detection, identification, and isolation in clinical, environmental, and food samples.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            is traditionally divided into at least 12 serotypes. Currently, there are no monoclonal antibodies (MAbs) available that are capable of binding to the surface of\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            strains representing all 12 serotypes. Such antibodies would be useful and are needed for the development of methods to detect and isolate\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            from food samples. In our study, we aimed to identify surface proteins that possess regions of well-conserved amino acid sequences among various serotypes and then to employ them as antigen targets (biomarkers) for the development of MAbs. Through bioinformatics and protein expression analysis, we identified one of the four putative surface protein candidates, LMOf2365_0639, encoded by the genome of the\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            serotype 4b strain F2365, as a useful surface biomarker. Extensive assessment of 35 MAbs raised against LMOf2365_0639 in our study revealed three MAbs (M3643, M3644, and M3651) that recognized a wide range of\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            isolates.\n          <\/jats:p>","DOI":"10.1128\/aem.00774-16","type":"journal-article","created":{"date-parts":[[2016,6,26]],"date-time":"2016-06-26T06:02:12Z","timestamp":1466920932000},"page":"5465-5476","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Identification of Surface Protein Biomarkers of Listeria monocytogenes via Bioinformatics and Antibody-Based Protein Detection Tools"],"prefix":"10.1128","volume":"82","author":[{"given":"Cathy X. 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