{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T12:24:55Z","timestamp":1782476695605,"version":"3.54.5"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2007,8,15]],"date-time":"2007-08-15T00:00:00Z","timestamp":1187136000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2007,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Prior research revealed that\n            <jats:italic>Polaromonas naphthalenivorans<\/jats:italic>\n            CJ2 carries and expresses genes encoding the gentisate metabolic pathway for naphthalene. These metabolic genes are split into two clusters, comprising\n            <jats:italic>nagRAaGHAbAcAdBFCQEDJI<\/jats:italic>\n            \u2032-\n            <jats:italic>orf1<\/jats:italic>\n            -\n            <jats:italic>tnpA<\/jats:italic>\n            and\n            <jats:italic>nagR2<\/jats:italic>\n            -\n            <jats:italic>orf2I<\/jats:italic>\n            \u2033\n            <jats:italic>KL<\/jats:italic>\n            (C. O. Jeon, M. Park, H. Ro, W. Park, and E. L. Madsen, Appl. Environ. Microbiol. 72:1086-1095, 2006). BLAST homology searches of sequences in GenBank indicated that the\n            <jats:italic>orf2<\/jats:italic>\n            gene from the small cluster likely encoded a salicylate 5-hydroxylase, presumed to catalyze the conversion of salicylate into gentisate. Here, we report physiological and genetic evidence that\n            <jats:italic>orf2<\/jats:italic>\n            does not encode salicylate 5-hydroxylase. Instead, we have found that\n            <jats:italic>orf2<\/jats:italic>\n            encodes 3-hydroxybenzoate 6-hydroxylase, the enzyme which catalyzes the NADH-dependent conversion of 3-hydroxybenzoate into gentisate. Accordingly, we have renamed\n            <jats:italic>orf2 nagX<\/jats:italic>\n            . After expression in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            , the NagX enzyme had an approximate molecular mass of 43 kDa, as estimated by gel filtration, and was probably a monomeric protein. The enzyme was able to convert 3-hydroxybenzoate into gentisate without salicylate 5-hydroxylase activity. Like other 3-hydroxybenzoate 6-hydroxylases, NagX utilized both NADH and NADPH as electron donors and exhibited a yellowish color, indicative of a bound flavin adenine dinucleotide. An engineered mutant of\n            <jats:italic>P. naphthalenivorans<\/jats:italic>\n            CJ2 defective in\n            <jats:italic>nagX<\/jats:italic>\n            failed to grow on 3-hydroxybenzoate but grew normally on naphthalene. These results indicate that the previously described small catabolic cluster in strain CJ2 may be multifunctional and is essential for the degradation of 3-hydroxybenzoate. Because\n            <jats:italic>nagX<\/jats:italic>\n            and an adjacent MarR-type regulatory gene are both closely related to homologues in\n            <jats:italic>Azoarcus<\/jats:italic>\n            species, this study raises questions about horizontal gene transfer events that contribute to operon evolution.\n          <\/jats:p>","DOI":"10.1128\/aem.00782-07","type":"journal-article","created":{"date-parts":[[2007,6,23]],"date-time":"2007-06-23T01:23:12Z","timestamp":1182561792000},"page":"5146-5152","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Molecular and Biochemical Characterization of 3-Hydroxybenzoate 6-Hydroxylase from\n            <i>Polaromonas naphthalenivorans<\/i>\n            CJ2"],"prefix":"10.1128","volume":"73","author":[{"given":"Minjeong","family":"Park","sequence":"first","affiliation":[{"name":"Division of Applied Life Science, EB-NCRC, PMBBRC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeji","family":"Jeon","sequence":"additional","affiliation":[{"name":"Division of Applied Life Science, EB-NCRC, PMBBRC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ho Hee","family":"Jang","sequence":"additional","affiliation":[{"name":"Division of Applied Life Science, EB-NCRC, PMBBRC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun-Su","family":"Ro","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Gyeongsang National University, Jinju 660-701"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Woojun","family":"Park","sequence":"additional","affiliation":[{"name":"Division of Environmental Science and Ecological Engineering, Korea University, Seoul 136-701, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugene L.","family":"Madsen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Cornell University, Ithaca, New York 14853-8101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Che Ok","family":"Jeon","sequence":"additional","affiliation":[{"name":"Division of Applied Life Science, EB-NCRC, 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