{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T02:34:14Z","timestamp":1787625254688,"version":"build-2736575974"},"reference-count":70,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["CREATE 509257-18"],"award-info":[{"award-number":["CREATE 509257-18"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2018-03892"],"award-info":[{"award-number":["RGPIN-2018-03892"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2024-04318"],"award-info":[{"award-number":["RGPIN-2024-04318"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"name":"German Research Council","award":["GRK 2172"],"award-info":[{"award-number":["GRK 2172"]}]},{"name":"German Research Council","award":["GRK 2172, INST 186\/1434-1"],"award-info":[{"award-number":["GRK 2172, INST 186\/1434-1"]}]},{"DOI":"10.13039\/501100004489","name":"Mitacs","doi-asserted-by":"publisher","award":["Globalink Research Award IT38888"],"award-info":[{"award-number":["Globalink Research Award IT38888"]}],"id":[{"id":"10.13039\/501100004489","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2026,7,22]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Plant pathogenic fungi pose a significant threat to global food security. Hence, discovering targeted (bio)chemical methods to combat plant diseases is a critical research avenue. Recent work has implicated copper radical oxidases (CROs) from Auxiliary Activity Family 5 (AA5) in fungal morphogenesis and pathogenesis, including in appressorium penetration by foliar phytopathogens in the genera\n                      <jats:italic toggle=\"yes\">Colletotrichum<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Magnaporthe<\/jats:italic>\n                      . We identified orthologous AA5-encoding genes in\n                      <jats:italic toggle=\"yes\">Verticillium<\/jats:italic>\n                      species; however, it was not clear that the functions of the corresponding CROs would be conserved in these vascular wilt pathogens. Using\n                      <jats:italic toggle=\"yes\">Verticillium dahliae<\/jats:italic>\n                      JR2 as an exemplar, recombinant protein production and detailed enzyme kinetic analyses revealed predominant aryl-alcohol oxidase activity of the AA5 subfamily 2 (AA5_2) homolog, and predominant aldehyde oxidase activity of the AA5 subfamily 1 (AA5_1) homolog. Transcriptomics and reverse genetics experiments on both CROs and three putative peroxidases\/catalases (known activators of CROs) in\n                      <jats:italic toggle=\"yes\">V. dahliae<\/jats:italic>\n                      JR2 failed to indicate direct roles in phytopathogenesis. Taken together, the data indicate that the biological roles of CROs and AA5_2 members, in particular, are not broadly conserved between foliar and vascular wilt phytopathogens. However, the biochemical characterization of an AA5_1 aldehyde oxidase and an AA5_2 aryl-alcohol oxidase from\n                      <jats:italic toggle=\"yes\">V. dahliae<\/jats:italic>\n                      , as well as an AA5_2 ortholog from\n                      <jats:italic toggle=\"yes\">V. longisporum<\/jats:italic>\n                      VL43, provides a foundation for further functional elucidation, as well as new biocatalysts for biotechnological applications.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Plant pathogens constitute a considerable burden to human society by attacking crops and reducing agricultural yields. Oxidative enzymes are often key weapons in the arsenal deployed by phytopathogens in the effort to breach cell walls and extract nutrients. Here, the characterization of the biochemical specificities of copper radical oxidases (CROs) from Verticillium wilt\/stripe fungi defines a range of possible alcohol and aldehyde substrates\n                        <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                        and outlines the potential of these enzymes for their biotechnological application for chemical valorization. Although specific gene knockouts did not reveal a biological function for these CROs, we now know that the molecular mechanism of CRO-mediated pathogenesis previously observed in foliar phytopathogens from the genera\n                        <jats:italic toggle=\"yes\">Colletotrichum<\/jats:italic>\n                        and\n                        <jats:italic toggle=\"yes\">Magnaporthe<\/jats:italic>\n                        is not conserved in the vascular wilt pathogen\n                        <jats:italic toggle=\"yes\">Verticillium<\/jats:italic>\n                        .\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/aem.00790-26","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T13:01:45Z","timestamp":1780491705000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Exploring the biochemical and biological functions of copper radical oxidases in the vascular wilt phytopathogen\n                    <i>Verticillium dahliae<\/i>"],"prefix":"10.1128","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-6194-2933","authenticated-orcid":false,"given":"Jessica K.","family":"Fong","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Michael Smith Laboratories, University of British Columbia","place":["Vancouver, Canada"]},{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Yu","family":"Chen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Microbiology and Genetics, Georg-August-Universit\u00e4t G\u00f6ttingen","place":["G\u00f6ttingen, Germany"]},{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"name":"G\u00f6ttingen Center for Molecular Biosciences (GZMB), Georg-August-Universit\u00e4t G\u00f6ttingen","place":["G\u00f6ttingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebekka","family":"Harting","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Microbiology and Genetics, Georg-August-Universit\u00e4t G\u00f6ttingen","place":["G\u00f6ttingen, Germany"]},{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"name":"G\u00f6ttingen Center for Molecular Biosciences (GZMB), Georg-August-Universit\u00e4t G\u00f6ttingen","place":["G\u00f6ttingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1017-5047","authenticated-orcid":false,"given":"Moritz","family":"Klein","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"name":"G\u00f6ttingen Center for Molecular Biosciences (GZMB), Georg-August-Universit\u00e4t G\u00f6ttingen","place":["G\u00f6ttingen, Germany"]},{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, Georg-August-Universit\u00e4t G\u00f6ttingen","place":["G\u00f6ttingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Lewandowski","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Microbiology and Genetics, Georg-August-Universit\u00e4t G\u00f6ttingen","place":["G\u00f6ttingen, Germany"]},{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"name":"G\u00f6ttingen Center for Molecular Biosciences (GZMB), Georg-August-Universit\u00e4t G\u00f6ttingen","place":["G\u00f6ttingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yann","family":"Mathieu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Michael Smith Laboratories, University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mireille","family":"Haon","sequence":"additional","affiliation":[{"name":"INRAE, Aix Marseille University, Biodiversit\u00e9 et Biotechnologie Fongiques (BBF)","place":["Marseille, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8354-3892","authenticated-orcid":true,"given":"Bastien","family":"Bissaro","sequence":"additional","affiliation":[{"name":"INRAE, Aix Marseille University, Biodiversit\u00e9 et Biotechnologie Fongiques (BBF)","place":["Marseille, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7570-3745","authenticated-orcid":true,"given":"Jean-Guy","family":"Berrin","sequence":"additional","affiliation":[{"name":"INRAE, Aix Marseille University, Biodiversit\u00e9 et Biotechnologie Fongiques (BBF)","place":["Marseille, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9888-7003","authenticated-orcid":false,"given":"Ivo","family":"Feussner","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"name":"G\u00f6ttingen Center for Molecular Biosciences (GZMB), Georg-August-Universit\u00e4t G\u00f6ttingen","place":["G\u00f6ttingen, Germany"]},{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, Georg-August-Universit\u00e4t G\u00f6ttingen","place":["G\u00f6ttingen, Germany"]},{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"name":"Service Unit for Metabolomics and Lipidomics, G\u00f6ttingen Center for Molecular Biosciences (GZMB), Georg-August-Universit\u00e4t G\u00f6ttingen","place":["G\u00f6ttingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3117-5626","authenticated-orcid":true,"given":"Gerhard H.","family":"Braus","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Microbiology and Genetics, Georg-August-Universit\u00e4t G\u00f6ttingen","place":["G\u00f6ttingen, Germany"]},{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"name":"G\u00f6ttingen Center for Molecular Biosciences (GZMB), Georg-August-Universit\u00e4t G\u00f6ttingen","place":["G\u00f6ttingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0101-862X","authenticated-orcid":true,"given":"Harry","family":"Brumer","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Michael Smith Laboratories, University of British Columbia","place":["Vancouver, Canada"]},{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, University of British Columbia","place":["Vancouver, Canada"]},{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Botany (Associate Member), University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s43016-020-0075-0"},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","unstructured":"International Plant Protection Convention Secretariat. 2021. Scientific review of the impact of climate change on plant pests \u2013 a global challenge to prevent and mitigate plant pest risks in agriculture forestry and ecosystems. Available from: https:\/\/doi.org\/10.4060\/cb4769en","DOI":"10.4060\/cb4769en"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-16-8877-5_23"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-phyto-080508-081748"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002137"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01714-20"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1463779"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1080\/07060661.2017.1375996"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2006.00323.x"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2013.00097"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005793"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/s42397-021-00111-6"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsoil.2025.106101"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-03-17-0083-RVW"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-phyto-102313-045831"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2022.114900"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwx089"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1045"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/1754-6834-6-41"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-017-2254-1"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2014.05.011"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20220124"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","unstructured":"Bissaro B Kodama S Hage H Haon M Grisel S Simaan A Beisson F Forget S Brumer H Rosso MN O\u2019Connell R Lafond M Kubo Y J. GB. 2021. Unravelling the role of alcohol copper radical oxidases in fungal plant pathogens. Res Sq. Res Sq . doi:10.21203\/rs.3.rs-493001\/v1","DOI":"10.21203\/rs.3.rs-493001\/v1"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.ade9982"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/cbe.5c00060"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evz040"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2019.101247"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.9b04727"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22137148"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10197"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-021-01984-0"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.01014-24"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.2.681"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof7080643"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00304-16"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40694-019-0067-8"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.3390\/catal8100476"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-004-1085-6"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1012431"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20260010"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-021-03981-w"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-324"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/tpg2.70120"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.1056008"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1199\/tab.0021"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2006.07.007"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10086-009-1105-6"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2025.05.017"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2019.103245"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btae620"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2020.10.024"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20122994"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-99-4890-1_6"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1111\/brv.12863"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.01876"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1111\/nph.15514"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1111\/2041-210X.13512"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-421"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-021-01156-3"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.01002"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.2144\/05393ST02"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mie.2022.08.015"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mie.2022.08.005"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0344-8"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-92"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/263910"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-014-0734-y"},{"key":"e_1_3_4_70_2","first-page":"770","article-title":"A modified freeze-thaw method for efficient transformation of Agrobacterium tumefaciens","volume":"93","author":"Jyothishwaran G","year":"2007","unstructured":"Jyothishwaran G, Kotresha D, Selvaraj T, Srideshikan SM, Rajvanshi PK, Jayabaskaran C. 2007. A modified freeze-thaw method for efficient transformation of Agrobacterium tumefaciens. Curr Sci 93:770\u2013772.","journal-title":"Curr Sci"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof8121252"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.00790-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.00790-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T13:06:42Z","timestamp":1784725602000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.00790-26"}},"subtitle":[],"editor":[{"given":"David A.","family":"Baltrus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,7,22]]},"references-count":70,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7,22]]}},"alternative-id":["10.1128\/aem.00790-26"],"URL":"https:\/\/doi.org\/10.1128\/aem.00790-26","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,22]]},"assertion":[{"value":"2026-04-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00790-26"}}