{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:18:48Z","timestamp":1791217128109,"version":"4.1.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T00:00:00Z","timestamp":1222819200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            (pneumococcus [Pnc]) is a causative agent of many infectious diseases, including pneumonia, septicemia, otitis media, and conjunctivitis. There have been documented conjunctivitis outbreaks in which nontypeable (NT), nonencapsulated Pnc has been identified as the etiological agent. The use of mass spectrometry to comparatively and differentially analyze protein and peptide profiles of whole-cell microorganisms remains somewhat uncharted. In this report, we discuss a comparative proteomic analysis between NT\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            conjunctivitis outbreak strains (cPnc) and other known typeable or NT pneumococcal and streptococcal isolates (including Pnc TIGR4 and R6,\n            <jats:italic>Streptococcus oralis<\/jats:italic>\n            ,\n            <jats:italic>Streptococcus mitis<\/jats:italic>\n            ,\n            <jats:italic>Streptococcus pseudopneumoniae<\/jats:italic>\n            , and\n            <jats:italic>Streptococcus pyogenes<\/jats:italic>\n            ) and nonstreptococcal isolates (including\n            <jats:italic>Escherichia coli<\/jats:italic>\n            ,\n            <jats:italic>Enterococcus faecalis<\/jats:italic>\n            , and\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            ) as controls. cPnc cells and controls were grown to mid-log phase, harvested, and subsequently treated with a 10% trifluoroacetic acid-sinapinic acid matrix mixture. Protein and peptide fragments of the whole-cell bacterial isolate-matrix combinations ranging in size from 2 to 14 kDa were evaluated by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Additionally Random Forest analytical tools and dendrogramic representations (Genesis) suggested similarities and clustered the isolates into distinct clonal groups, respectively. Also, a peak list of protein and peptide masses was obtained and compared to a known Pnc protein mass library, in which a peptide common and unique to cPnc isolates was tentatively identified. Information gained from this study will lead to the identification and validation of proteins that are commonly and exclusively expressed in cPnc strains which could potentially be used as a biomarker in the rapid diagnosis of pneumococcal conjunctivitis.\n          <\/jats:p>","DOI":"10.1128\/aem.00791-08","type":"journal-article","created":{"date-parts":[[2008,8,16]],"date-time":"2008-08-16T00:26:05Z","timestamp":1218846365000},"page":"5891-5897","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["Differentiation of\n            <i>Streptococcus pneumoniae<\/i>\n            Conjunctivitis Outbreak Isolates by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry"],"prefix":"10.1128","volume":"74","author":[{"given":"Yulanda M.","family":"Williamson","sequence":"first","affiliation":[{"name":"National Center for Immunizations and Respiratory Diseases"},{"name":"National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hercules","family":"Moura","sequence":"additional","affiliation":[{"name":"National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian R.","family":"Woolfitt","sequence":"additional","affiliation":[{"name":"National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James L.","family":"Pirkle","sequence":"additional","affiliation":[{"name":"National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Barr","sequence":"additional","affiliation":[{"name":"National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Da Gloria","family":"Carvalho","sequence":"additional","affiliation":[{"name":"National Center for Immunizations and Respiratory Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin P.","family":"Ades","sequence":"additional","affiliation":[{"name":"National Center for Immunizations and Respiratory Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George M.","family":"Carlone","sequence":"additional","affiliation":[{"name":"National Center for Immunizations and Respiratory Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacquelyn S.","family":"Sampson","sequence":"additional","affiliation":[{"name":"National Center for Immunizations and Respiratory Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac010651t"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1010933404324"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.9.4415-4417.2003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02498-06"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2006.03.024"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/mas.10004"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00556-07"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(81)80754-8"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(19960731)10:10<1227::AID-RCM659>3.0.CO;2-6"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.19.5709-5717.2001"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac990832j"},{"key":"e_1_3_2_13_2","first-page":"1586","volume":"13","year":"2000","unstructured":"Jarman, K. 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Njenga, P. Lewa, J. Obiero, H. Otieno, J. S. Sampson, and G. M. Carlone. 2005. Diagnosis of invasive pneumococcal disease among children in Kenya with enzyme-linked immunosorbent assay for immunoglobulin G antibodies to pneumococcal surface adhesin A. Clin. Diagn. Lab. Immunol.12:1195-1201.","journal-title":"Clin. Diagn. Lab. 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