{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T15:20:36Z","timestamp":1774279236162,"version":"3.50.1"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"15","license":[{"start":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T00:00:00Z","timestamp":1343779200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            This work reports on the identification and molecular characterization of the two-component regulatory system (2CRS) PhoRP, which controls the response to inorganic phosphate (P\n            <jats:sub>i<\/jats:sub>\n            ) starvation in\n            <jats:named-content content-type=\"genus-species\">Bifidobacterium breve<\/jats:named-content>\n            UCC2003. The response regulator PhoP was shown to bind to the promoter region of\n            <jats:named-content content-type=\"genus-species\">pstSCAB<\/jats:named-content>\n            , specifying a predicted P\n            <jats:sub>i<\/jats:sub>\n            transporter system, as well as that of\n            <jats:named-content content-type=\"genus-species\">phoU<\/jats:named-content>\n            , which encodes a putative P\n            <jats:sub>i<\/jats:sub>\n            -responsive regulatory protein. This interaction is assumed to cause transcriptional modulation under conditions of P\n            <jats:sub>i<\/jats:sub>\n            limitation. Our data suggest that the\n            <jats:italic>phoRP<\/jats:italic>\n            genes are subject to positive autoregulation and, together with\n            <jats:italic>pstSCAB<\/jats:italic>\n            and presumably\n            <jats:italic>phoU<\/jats:italic>\n            , represent the complete regulon controlled by the\n            <jats:italic>phoRP<\/jats:italic>\n            -encoded 2CRS in\n            <jats:named-content content-type=\"genus-species\">B. breve<\/jats:named-content>\n            UCC2003. Determination of the minimal PhoP binding region combined with bioinformatic analysis revealed the probable recognition sequence of PhoP, designated here as the PHO box, which together with\n            <jats:italic>phoRP<\/jats:italic>\n            is conserved among many high-GC-content Gram-positive bacteria. The importance of the\n            <jats:italic>phoRP<\/jats:italic>\n            2CRS in the response of\n            <jats:named-content content-type=\"genus-species\">B. breve<\/jats:named-content>\n            to P\n            <jats:sub>i<\/jats:sub>\n            starvation conditions was confirmed by analysis of a\n            <jats:named-content content-type=\"genus-species\">B. breve<\/jats:named-content>\n            <jats:italic>phoP<\/jats:italic>\n            insertion mutant which exhibited decreased growth under phosphate-limiting conditions compared to its parent strain UCC2003.\n          <\/jats:p>","DOI":"10.1128\/aem.00804-12","type":"journal-article","created":{"date-parts":[[2012,5,26]],"date-time":"2012-05-26T04:48:54Z","timestamp":1338007734000},"page":"5258-5269","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["A Conserved Two-Component Signal Transduction System Controls the Response to Phosphate Starvation in Bifidobacterium breve UCC2003"],"prefix":"10.1128","volume":"78","author":[{"given":"Pablo","family":"Alvarez-Martin","sequence":"first","affiliation":[{"name":"Alimentary Pharmabiotic Centre, National University of Ireland, Cork, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matilde","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Alimentary Pharmabiotic Centre, National University of Ireland, Cork, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary","family":"O'Connell-Motherway","sequence":"additional","affiliation":[{"name":"Alimentary Pharmabiotic Centre, National University of Ireland, Cork, Ireland"},{"name":"Department of Microbiology, National University of Ireland, Cork, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerry Joan","family":"O'Connell","sequence":"additional","affiliation":[{"name":"Alimentary Pharmabiotic Centre, National 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