{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T22:48:16Z","timestamp":1791067696905,"version":"4.1.0"},"reference-count":78,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2016,7,15]],"date-time":"2016-07-15T00:00:00Z","timestamp":1468540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000052","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI102584"],"award-info":[{"award-number":["AI102584"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2016,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The ability to sense and adapt to temperature fluctuation is critical to the aquatic survival, transmission, and infectivity of\n            <jats:named-content content-type=\"genus-species\">Vibrio cholerae<\/jats:named-content>\n            , the causative agent of the disease cholera. Little information is available on the physiological changes that occur when\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            experiences temperature shifts. The genome-wide transcriptional profile of\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            upon a shift in human body temperature (37\u00b0C) to lower temperatures, 15\u00b0C and 25\u00b0C, which mimic those found in the aquatic environment, was determined. Differentially expressed genes included those involved in the cold shock response, biofilm formation, type VI secretion, and virulence. Analysis of a mutant lacking the cold shock gene\n            <jats:italic>cspV<\/jats:italic>\n            , which was upregulated &gt;50-fold upon a low-temperature shift, revealed that it regulates genes involved in biofilm formation and type VI secretion. CspV controls biofilm formation through modulation of the second messenger cyclic diguanylate and regulates type VI-mediated interspecies killing in a temperature-dependent manner. Furthermore, a strain lacking\n            <jats:italic>cspV<\/jats:italic>\n            had significant defects for attachment and type VI-mediated killing on the surface of the aquatic crustacean\n            <jats:named-content content-type=\"genus-species\">Daphnia magna<\/jats:named-content>\n            . Collectively, these studies reveal that\n            <jats:italic>cspV<\/jats:italic>\n            is a major regulator of the temperature downshift response and plays an important role in controlling cellular processes crucial to the infectious cycle of\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            .\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            Little is known about how human pathogens respond and adapt to ever-changing parameters of natural habitats outside the human host and how environmental adaptation alters dissemination.\n            <jats:named-content content-type=\"genus-species\">Vibrio cholerae<\/jats:named-content>\n            , the causative agent of the severe diarrheal disease cholera, experiences fluctuations in temperature in its natural aquatic habitats and during the infection process. Furthermore, temperature is a critical environmental signal governing the occurrence of\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            and cholera outbreaks. In this study, we showed that\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            reprograms its transcriptome in response to fluctuations in temperature, which results in changes to biofilm formation and type VI secretion system activation. These processes in turn impact environmental survival and the virulence potential of this pathogen.\n          <\/jats:p>","DOI":"10.1128\/aem.00807-16","type":"journal-article","created":{"date-parts":[[2016,5,23]],"date-time":"2016-05-23T17:00:57Z","timestamp":1464022857000},"page":"4441-4452","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":74,"title":["Response of Vibrio cholerae to Low-Temperature Shifts: CspV Regulation of Type VI Secretion, Biofilm Formation, and Association with Zooplankton"],"prefix":"10.1128","volume":"82","author":[{"given":"Loni","family":"Townsley","sequence":"first","affiliation":[{"name":"Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilou P.","family":"Sison Mangus","sequence":"additional","affiliation":[{"name":"Department of Ocean Sciences and Institute for Marine Sciences, University of California, Santa Cruz, Santa Cruz, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjin","family":"Mehic","sequence":"additional","affiliation":[{"name":"Department of Ocean Sciences and Institute for Marine Sciences, University of California, Santa Cruz, Santa Cruz, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fitnat H.","family":"Yildiz","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.4.2849-2855.2006"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.4.1301-1314.1998"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.4.757-770.2002"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2006.01011.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2004.00601.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.8.4645-4654.2005"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.6.3676-3680.2003"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.5.2773-2785.2003"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12246"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.89"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2009.50"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.24.9898"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01297-06"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1411570111"},{"key":"e_1_3_3_16_2","first-page":"211","article-title":"Cold shock response and low temperature adaptation in psychrotrophic bacteria","volume":"1","author":"H\u00e9braud M","year":"1999","unstructured":"H\u00e9braud M, Potier P. 1999. Cold shock response and low temperature adaptation in psychrotrophic bacteria. J Mol Microbiol Biotechnol 1:211\u2013219.","journal-title":"J Mol Microbiol Biotechnol"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.20.7007-7014.2004"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2009.11.048"},{"key":"e_1_3_3_19_2","first-page":"125","article-title":"Recent developments in bacterial cold-shock response","volume":"6","author":"Phadtare S","year":"2004","unstructured":"Phadtare S. 2004. Recent developments in bacterial cold-shock response. Curr Issues Mol Biol 6:125\u2013136.","journal-title":"Curr Issues Mol Biol"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.4161\/rna.7.6.13482"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02000-07"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.11.6361-6369.2003"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12799"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2008.01714.x"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0237386100"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.45.1.275-283.1983"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00260"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601277103"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.040196-0"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.7.4028"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.3.1049-1059.2006"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01569-06"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07867.x"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02439-14"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00429-09"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.10.3600-3613.2006"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04155.x"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01756-07"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.01.042"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510322103"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004933"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1260064"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10846"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0706532104"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023876"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006734"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05414-11"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1014998107"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05510-11"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01750-05"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2004-5-10-r80"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.091062498"},{"key":"e_1_3_3_53_2","first-page":"87","volume-title":"A\u2013Z of quantitative PCR","author":"Pfaffl MW","year":"2004","unstructured":"Pfaffl MW. 2004. Quantification strategies in real-time PCR, p 87\u2013112. In Bustin SA (ed), A\u2013Z of quantitative PCR. International University Line, La Jolla, CA."},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-146-10-2395"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.7.3220-3225.2001"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.8.2370-2373.1990"},{"key":"e_1_3_3_57_2","first-page":"1","article-title":"Assessment of Daphnia, Moina and Cylops in freshwater ecosystems and the evaluation of mixed culture in laboratory","volume":"3","author":"Miah MF","year":"2013","unstructured":"Miah MF, Roy S, Jinnat E, Khan ZK. 2013. Assessment of Daphnia, Moina and Cylops in freshwater ecosystems and the evaluation of mixed culture in laboratory. Am Int J Res Form Appl Nat Sci 3:1\u20137.","journal-title":"Am Int J Res Form Appl Nat Sci"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2014.116"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.22.6395-6402.2002"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1758-2229.2010.00229.x"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01908-05"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10126-010-9356-1"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2008.01434.x"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2004.11.005"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature00778"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00584-08"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45990-0"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.2.1613-1622.2006"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044104"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/012021-0"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1181185"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05167-11"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01215.x"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp121"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.104.1.410-433.1970"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308707101"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1120096"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004066"},{"key":"e_1_3_3_79_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00034-10"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00807-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00807-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T18:13:49Z","timestamp":1645640029000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.00807-16"}},"subtitle":[],"editor":[{"given":"J.","family":"Bj\u00f6rkroth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,7,15]]},"references-count":78,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2016,7,15]]}},"alternative-id":["10.1128\/AEM.00807-16"],"URL":"https:\/\/doi.org\/10.1128\/aem.00807-16","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,15]]},"assertion":[{"value":"2016-03-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-05-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-06-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}