{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:57:43Z","timestamp":1785416263832,"version":"3.56.0"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:00:00Z","timestamp":1761091200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:00:00Z","timestamp":1761091200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2025,10,22]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The guts of animals and humans harbor diverse microbial communities that are regularly exposed to bacteria originating from food, water, and their surroundings. Species such as\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      are adept at colonizing multiple hosts, along with surviving in the environment. By encoding pathogenic traits and transmissible forms of antimicrobial resistance (AMR),\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      can also pose a zoonotic risk. Our understanding of the factors that govern host residency is limited. Here, we used a chicken cecal fermentation model to study survival and the AMR transfer potential of 17 host-associated extended-spectrum \u03b2-lactamase (ESBL)-producing\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      isolates. Vessels containing chicken cecal contents were stabilized for 4 days before the addition of a cocktail comprising ESBL-producing\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      obtained from human, cattle, pig, and chicken hosts. Consecutive sampling showed that pig and cattle-associated isolates persisted in most vessels, although the recovery of all isolates declined over time. Increasing the inoculum dose or adding ceftiofur helped to stabilize populations of ESBL\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      within the vessels, although this did not result in outgrowth of resistant populations in all vessels. Sequencing revealed that most new ESBL-producing\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      recovered during the study acquired a\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>CTX-M-1<\/jats:sub>\n                      plasmid from a single ESBL\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      included in the cocktail that lacked host-specific traits (generalist). Our data highlight that isolate-specific differences in the\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      genome composition likely explain the persistence of specific clones and efficiency of plasmid transfer, both of which could impact the spread of AMR in complex communities.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        There are few insights into how host-associated\n                        <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                        behave within the gut environment of other hosts.\n                        <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                        isolates that are immigrants to the gastrointestinal system of humans and animals have the potential to transfer their resistance to other native bacteria. A better understanding of this process is needed to assess how the gastrointestinal environment could serve as a reservoir and a melting pot of new, multidrug-resistant\n                        <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                        isolates.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/aem.00822-25","type":"journal-article","created":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T13:00:11Z","timestamp":1758286811000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Evidence of ESBL plasmid transfer and selective persistence of multiple host-associated\n                    <i>Escherichia coli<\/i>\n                    isolates in a chicken cecal fermentation model"],"prefix":"10.1128","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8347-0339","authenticated-orcid":true,"given":"J.","family":"Leng","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00ks66431","id-type":"ROR","asserted-by":"publisher"}],"name":"Faculty of Health and Medical Sciences, School of Veterinary Medicine, University of Surrey","place":["Guildford, United Kingdom"]},{"id":[{"id":"https:\/\/ror.org\/01kwjhv40","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Integrative Biology, University of Liverpool","place":["Liverpool, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3185-1046","authenticated-orcid":true,"given":"M.","family":"Ferrandis-Vila","sequence":"additional","affiliation":[{"name":"Friedrich-Loeffler-Institut, Institute of Molecular Pathogenesis","place":["Jena, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2245-6987","authenticated-orcid":true,"given":"R.","family":"Oldenkamp","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/008xxew50","id-type":"ROR","asserted-by":"publisher"}],"name":"Faculty of Science, Amsterdam Institute for Life and Environment (A-LIFE), Vrije Universiteit Amsterdam","place":["Amsterdam, the Netherlands"]},{"id":[{"id":"https:\/\/ror.org\/04dkp9463","id-type":"ROR","asserted-by":"publisher"}],"name":"Amsterdam Institute for Global Health and Development (AIGHD), University of Amsterdam","place":["Amsterdam, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9563-6348","authenticated-orcid":true,"given":"J. W.","family":"Mehat","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00ks66431","id-type":"ROR","asserted-by":"publisher"}],"name":"Faculty of Health and Medical Sciences, School of Biosciences, University of Surrey","place":["Guildford, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. S.","family":"Fivian-Hughes","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00ks66431","id-type":"ROR","asserted-by":"publisher"}],"name":"Faculty of Health and Medical Sciences, School of Biosciences, University of Surrey","place":["Guildford, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3615-447X","authenticated-orcid":true,"given":"S.","family":"Kumar Tiwari","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01k5qnb77","id-type":"ROR","asserted-by":"publisher"}],"name":"Genome Sequencing and Genomic Epidemiology, Robert Koch Institute","place":["Berlin, Germany"]},{"id":[{"id":"https:\/\/ror.org\/04td3ys19","id-type":"ROR","asserted-by":"publisher"}],"name":"Gut Microbes and Health Institute Strategic Program, Quadram Institute Bioscience, Norwich Research Park","place":["Norwich, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Van der Putten","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04dkp9463","id-type":"ROR","asserted-by":"publisher"}],"name":"Amsterdam UMC, University of Amsterdam","place":["Amsterdam, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Trung Nguyen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05rehad94","id-type":"ROR","asserted-by":"publisher"}],"name":"Oxford University Clinical Research Unit Vietnam","place":["Ho Chi Minh City, Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Bethe","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/046ak2485","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Microbiology and Epizootics, School of Veterinary Medicine, Freie Universit\u00e4t Berlin","place":["Berlin, Germany"]},{"id":[{"id":"https:\/\/ror.org\/046ak2485","id-type":"ROR","asserted-by":"publisher"}],"name":"Veterinary Centre for Resistance Research (TZR), School of Veterinary Medicine, Freie Universit\u00e4t Berlin","place":["Berlin, Germany"]},{"id":[{"id":"https:\/\/ror.org\/0329ynx05","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Environmental Hygiene, German Environment Agency","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Clark","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00xkqe770","id-type":"ROR","asserted-by":"publisher"}],"name":"Epsom and St Helier University Hospitals NHS Trust","place":["Epsom, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Singh","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01v13p275","id-type":"ROR","asserted-by":"publisher"}],"name":"Watford General Hospital","place":["Watford, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2225-7267","authenticated-orcid":true,"given":"T.","family":"Semmler","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01k5qnb77","id-type":"ROR","asserted-by":"publisher"}],"name":"Genome Sequencing and Genomic Epidemiology, Robert Koch Institute","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6303-8212","authenticated-orcid":true,"given":"S.","family":"Schwarz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/046ak2485","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Microbiology and Epizootics, School of Veterinary Medicine, Freie Universit\u00e4t Berlin","place":["Berlin, Germany"]},{"id":[{"id":"https:\/\/ror.org\/046ak2485","id-type":"ROR","asserted-by":"publisher"}],"name":"Veterinary Centre for Resistance Research (TZR), School of Veterinary Medicine, Freie Universit\u00e4t Berlin","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Alvarez","sequence":"additional","affiliation":[{"name":"VISAVET, Health Surveillance Centre, Complutense University","place":["Madrid, Spain"]},{"id":[{"id":"https:\/\/ror.org\/024409k12","id-type":"ROR","asserted-by":"publisher"}],"name":"Depatment of Animal Health, Faculty of Veterinary Medicine, Complutense University of Madrid","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9320-4050","authenticated-orcid":true,"given":"N. T.","family":"Hoa","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05rehad94","id-type":"ROR","asserted-by":"publisher"}],"name":"Oxford University Clinical Research Unit Vietnam","place":["Ho Chi Minh City, Vietnam"]},{"id":[{"id":"https:\/\/ror.org\/052gg0110","id-type":"ROR","asserted-by":"publisher"}],"name":"Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford","place":["Oxford, United Kingdom"]},{"id":[{"id":"https:\/\/ror.org\/003g49r03","id-type":"ROR","asserted-by":"publisher"}],"name":"Biomedical Research Center, Pham Ngoc Thach University of Medicine","place":["Ho Chi Minh City, Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Bootsma","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04pp8hn57","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Mathematics, Faculty of Sciences, Utrecht University","place":["Utrecht, the Netherlands"]},{"id":[{"id":"https:\/\/ror.org\/04pp8hn57","id-type":"ROR","asserted-by":"publisher"}],"name":"University Medical Center Utrecht, Utrecht University","place":["Utrecht, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0049-1371","authenticated-orcid":true,"given":"C.","family":"Menge","sequence":"additional","affiliation":[{"name":"Friedrich-Loeffler-Institut, Institute of Molecular Pathogenesis","place":["Jena, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7500-5864","authenticated-orcid":true,"given":"C.","family":"Berens","sequence":"additional","affiliation":[{"name":"Friedrich-Loeffler-Institut, Institute of Molecular Pathogenesis","place":["Jena, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Schultsz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04dkp9463","id-type":"ROR","asserted-by":"publisher"}],"name":"Amsterdam UMC, University of Amsterdam","place":["Amsterdam, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1244-3082","authenticated-orcid":true,"given":"J. M.","family":"Ritchie","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00ks66431","id-type":"ROR","asserted-by":"publisher"}],"name":"Faculty of Health and Medical Sciences, School of Biosciences, University of Surrey","place":["Guildford, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5861-613X","authenticated-orcid":true,"given":"R. M.","family":"La Ragione","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00ks66431","id-type":"ROR","asserted-by":"publisher"}],"name":"Faculty of Health and Medical Sciences, School of Veterinary Medicine, University of Surrey","place":["Guildford, United Kingdom"]},{"id":[{"id":"https:\/\/ror.org\/00ks66431","id-type":"ROR","asserted-by":"publisher"}],"name":"Faculty of Health and Medical Sciences, School of Biosciences, University of Surrey","place":["Guildford, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1596\/31369"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2016.13"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069621"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-019-0435-7"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1323632111"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.MBP-0006-2014"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-38628-1"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.69756"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/35012500"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.15698\/mic2018.05.628"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature17626"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00972-09"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/ecosalplus.ESP-0003-2020"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2012.03850.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02693-18"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dky506"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075392"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-023-01562-w"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2015.06.007"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2013.02.008"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa399"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X.JFP-10-372"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2014.02.023"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.11-0372"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01756"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/jfs.12282"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00204-13"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2019.108357"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2010.04907.x"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00777-17"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-022-02508-y"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuac008"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2012.03616.x"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.716299"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.3109\/03009734.2014.904457"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.mtbp-0016-2017"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-018-0224-8"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-019-0483-z"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks368"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-023-04716-2"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000465"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-020-00832-7"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000203"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2018.06.003"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks308"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01014-08"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03497.x"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-041320-031627"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1986.tb01073.x"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1017\/s002217240001158x"},{"key":"e_1_3_3_52_2","unstructured":"Blanchet F Kindt R Legendre P Minchin P Solymos P Stevens M Szoecs E Wagner H Barbour M Bedward M et al.. 2024. vegan: community ecology package vR package version 2.7-0. https:\/\/github.com\/vegandevs\/vegan."},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24277-4"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0209-9"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3869"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03006-05"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.14826.1"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0524-x"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.00822-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.00822-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T13:23:07Z","timestamp":1761139387000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.00822-25"}},"subtitle":[],"editor":[{"given":"Danilo","family":"Ercolini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,10,22]]},"references-count":58,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10,22]]}},"alternative-id":["10.1128\/aem.00822-25"],"URL":"https:\/\/doi.org\/10.1128\/aem.00822-25","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,22]]},"assertion":[{"value":"2025-05-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00822-25"}}