{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:20:06Z","timestamp":1790317206084,"version":"4.1.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2006,8,1]],"date-time":"2006-08-01T00:00:00Z","timestamp":1154390400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Multiple reductive dehalogenase (RDase), hydrogenase (H\n            <jats:sub>2<\/jats:sub>\n            ase), and other respiration-associated (RA) oxidoreductase genes have been identified in cultured representatives of\n            <jats:italic>Dehalococcoides<\/jats:italic>\n            . Although their products are likely to play key roles in the environmentally important process of reductive dechlorination, very little information is available about their regulation and specific functions. Here we show increased expression and temporal variability in the expression of five RDase genes and in the expression of genes for a putative formate dehydrogenase (Fdh) and two H\n            <jats:sub>2<\/jats:sub>\n            ases, including a periplasmic [Ni\/Fe] H\n            <jats:sub>2<\/jats:sub>\n            ase (Hup) and a cytoplasmic [Fe] H\n            <jats:sub>2<\/jats:sub>\n            ase (Vhu). mRNA transcripts extracted from tetrachloroethene-dechlorinating mixed cultures corresponding to Fdh, the H\n            <jats:sub>2<\/jats:sub>\n            ase Hup, and the RDase targets TceA and DET0162 were expressed most highly, with average levels 34 (\u00b1 7.5)-, 23 (\u00b1 6.7)-, 16 (\u00b1 3.3)-, and 13 (\u00b1 3.3)-fold higher, respectively, than that for RNA polymerase (RpoB). H\n            <jats:sub>2<\/jats:sub>\n            ase and RA transcripts reached their respective expression maxima within the first 2 h after feeding. RDase transcripts, however, were most highly expressed after 3 h and exhibited greater temporal variability than other transcripts. Comparison with\n            <jats:italic>D. ethenogenes<\/jats:italic>\n            strain 195 pure culture expression levels indicated that RDase DET1545 was more highly expressed in mixed cultures, where, on average, its transcript level was sixfold higher than that of RpoB. While the specific functions of several of these gene products remain elusive, the high expression levels and temporal variability reported here suggest that these groups of enzymes are metabolically important for the respiration of chlorinated ethenes in mixed cultures containing\n            <jats:italic>Dehalococcoides<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aem.00855-06","type":"journal-article","created":{"date-parts":[[2006,8,2]],"date-time":"2006-08-02T23:53:37Z","timestamp":1154562817000},"page":"5486-5491","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":75,"title":["Temporal Expression of Respiratory Genes in an Enrichment Culture Containing\n            <i>Dehalococcoides ethenogenes<\/i>"],"prefix":"10.1128","volume":"72","author":[{"given":"Brian G.","family":"Rahm","sequence":"first","affiliation":[{"name":"Department of Civil and Environmental Engineering, 220 Hollister Hall, Cornell University, Ithaca, New York 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Morris","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, 220 Hollister Hall, Cornell University, Ithaca, New York 14853"},{"name":"Department of Microbiology, Wing Hall, Cornell University, Ithaca, New York 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth E.","family":"Richardson","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, 220 Hollister Hall, Cornell University, Ithaca, New York 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Agency for Toxic Substances and Disease Registry. 1996. ToxFAQs for chlorinated ethenes. [Online.] http:\/\/www.atsdr.cdc.gov\/tfacts70.html\n."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.11.3622-3629.1992"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.8.2287-2292.1991"},{"key":"e_1_3_2_6_2","unstructured":"Fennell D. E. 1998. Ph.D. thesis. Cornell University Ithaca N.Y."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/es960756r"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.11.6870-6877.2005"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.9.5290-5297.2004"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.7.3866-3871.2005"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.11.7145-7151.2005"},{"key":"e_1_3_2_12_2","first-page":"1269","volume":"23","year":"2005","unstructured":"Kube, M., A. Beck, S. H. Zinder, H. Kuhl, R. Reinhardt, and L. Adrian. 2005. Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1. Nature23:1269-1273.","journal-title":"Nature"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.12.5141-5147.2000"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.4.1270-1275.1998"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.7.3108-3113.1999"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.11.3928-3933.1995"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5318.1568"},{"key":"e_1_3_2_18_2","unstructured":"Environ. Microbiol."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.3.1664-1667.2005"},{"key":"e_1_3_2_20_2","first-page":"365","volume":"132","year":"2000","unstructured":"Rozen, S., and H. J. Skaletsky. 2000. Primer3 on the WWW for general users and for biologist programmers. Methods Mol. Biol.132:365-386.","journal-title":"Methods Mol. Biol."},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1102226"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1021\/es9602455"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.12.8257-8264.2005"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00855-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00855-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T16:21:30Z","timestamp":1645633290000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.00855-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,8]]},"references-count":22,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2006,8]]}},"alternative-id":["10.1128\/AEM.00855-06"],"URL":"https:\/\/doi.org\/10.1128\/aem.00855-06","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,8]]},"assertion":[{"value":"2006-04-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-06-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}