{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:18:43Z","timestamp":1791249523692,"version":"4.1.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2013,8,15]],"date-time":"2013-08-15T00:00:00Z","timestamp":1376524800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2013,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Antimicrobial resistance of\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            to modern beta-lactam antibiotics due to the production of extended-spectrum beta-lactamases (ESBL) and\/or plasmid-mediated AmpC beta-lactamases (AmpC) represents an emerging and increasing resistance problem that dramatically limits therapeutic options in both human and veterinary medicine. The presence of ESBL\/AmpC genes in commensal\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            from food-producing animals like broilers may pose a human health hazard. However, there are no data available concerning the prevalence of ESBL\/AmpC-producing\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            in German broiler flocks using selective methods. In this longitudinal study, samples were taken from seven conventional broiler fattening farms at three different times within one fattening period. Various samples originating from the animals as well as from their direct environment in the barn were investigated for the occurrence of ESBL\/AmpC-producing\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            . Average detection levels of 51, 75, and 76% in animal samples collected during the three samplings in the course of the fattening period demonstrate a colonization of even 1-day-old chicks, as well as a continuous significant (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001) increase in prevalence thereafter. The detection frequencies in housing environmental samples were relatively high, with an increase over time, and ranged between 54.2 and 100%. A total of 359\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            isolates were characterized by PCR and partly via the disc diffusion method. This study shows that prevalence of ESBL\/AmpC-producing\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            increases during the fattening period of the broiler flocks examined. Both colonized day-old chicks and contaminated farm environments could represent significant sources of ESBL\/AmpC-producing\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            in German broiler fattening farms.\n          <\/jats:p>","DOI":"10.1128\/aem.00856-13","type":"journal-article","created":{"date-parts":[[2013,6,8]],"date-time":"2013-06-08T01:09:17Z","timestamp":1370653757000},"page":"4815-4820","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":129,"title":["Longitudinal Monitoring of Extended-Spectrum-Beta-Lactamase\/AmpC-Producing Escherichia coli at German Broiler Chicken Fattening Farms"],"prefix":"10.1128","volume":"79","author":[{"given":"H.","family":"Laube","sequence":"first","affiliation":[{"name":"Institute for Animal Hygiene and Environmental Health, Free University Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Friese","sequence":"additional","affiliation":[{"name":"Institute for Animal Hygiene and Environmental Health, Free University Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"von Salviati","sequence":"additional","affiliation":[{"name":"Institute for Animal Hygiene and Environmental Health, Free University Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Guerra","sequence":"additional","affiliation":[{"name":"Federal Institute for Risk Assessment, Department Biological Safety, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"K\u00e4sbohrer","sequence":"additional","affiliation":[{"name":"Federal Institute for Risk Assessment, Department Biological Safety, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Kreienbrock","sequence":"additional","affiliation":[{"name":"Institute for Biometrics, Epidemiology and Information Processing, Veterinary University Hannover, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.","family":"Roesler","sequence":"additional","affiliation":[{"name":"Institute for Animal Hygiene and Environmental Health, Free University Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","unstructured":"Danish Integrated Antimicrobial Resistance Monitoring and Research Programme (DANMAP). 2011. 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