{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T02:08:14Z","timestamp":1792202894399,"version":"4.4.0"},"reference-count":84,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100006759","name":"California Department of Food and Agriculture","doi-asserted-by":"publisher","award":["24-0001-028-SF"],"award-info":[{"award-number":["24-0001-028-SF"]}],"id":[{"id":"10.13039\/100006759","id-type":"DOI","asserted-by":"publisher"}]},{"name":"United States Department of Agriculture, HATCH","award":["1026705"],"award-info":[{"award-number":["1026705"]}]},{"name":"TEX0-9621, AgriLife IVD","award":["114190-96210"],"award-info":[{"award-number":["114190-96210"]}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2017-70016-26053"],"award-info":[{"award-number":["2017-70016-26053"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2020-70029-33202"],"award-info":[{"award-number":["2020-70029-33202"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2025,8,20]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n Huanglongbing (HLB) is a devastating citrus disease associated with the gram-negative, phloem-limited, and unculturable bacterium \u201c\n Candidatus<\/jats:italic>\n Liberibacter asiaticus (\n C<\/jats:italic>\n Las),\u201d which is transmitted by the Asian citrus psyllid\n Diaphorina citri<\/jats:italic>\n . Despite extensive research, effective, long-term, and sustainable solutions for managing HLB remain elusive. Oxytetracycline (OTC) is currently used as an emergency measure, but there is an urgent need for alternative compounds to complement or replace OTC. In this study, we identified amicoumacins, a class of antimicrobial compounds produced by the bacterium\n Bacillus safensis<\/jats:italic>\n CB729 isolated from the citrus microbiome, and demonstrated their ability to suppress\n C<\/jats:italic>\n Las. Genome mining of\n B. safensis<\/jats:italic>\n CB729, combined with metabolomic analysis and bioassay-guided fractionation, revealed the presence of amicoumacins and related derivatives in fractions inhibitory to\n Liberibacter crescens<\/jats:italic>\n , a culturable surrogate for\n C<\/jats:italic>\n Las. We tested commercially available synthetic amicoumacins A and B, along with a\n B. safensis<\/jats:italic>\n -derived amicoumacin mixture, against\n L. crescens<\/jats:italic>\n and\n C<\/jats:italic>\n Las. We determined the MICs of amicoumacin A (1.25 \u00b5g\/mL) and amicoumacin B (10 \u00b5g\/mL) against\n L. crescens<\/jats:italic>\n . Furthermore, amicoumacin B and the amicoumacin mixture significantly reduced\n C<\/jats:italic>\n Las populations in\n ex vivo<\/jats:italic>\n citrus hairy root assays. This study highlights the potential of amicoumacins as a promising group of natural products for the management of HLB, offering valuable insights for the development of novel and sustainable disease control strategies.\n <\/jats:p>\n \n IMPORTANCE<\/jats:title>\n \n For two decades, the citrus industry has been severely impacted by Huanglongbing (HLB), a devastating disease caused by \u201c\n Candidatus<\/jats:italic>\n Liberibacter asiaticus (\n C<\/jats:italic>\n Las)\u201d and transmitted by the Asian citrus psyllid (\n Diaphorina citri<\/jats:italic>\n ). Despite extensive research, effective, long-term, and sustainable solutions remain unavailable for growers. Currently, medically relevant antibiotics, such as oxytetracycline (OTC), are used as an emergency response to combat HLB in Florida, the most affected citrus-producing state in the U.S. This underscores the urgent need for alternative treatments that can be used in rotation or as replacements for OTC. Here, we present amicoumacins, a group of bioactive secondary metabolites with antibiotic properties. We identified amicoumacin B and its derivatives from the culture broth of a\n Bacillus safensis<\/jats:italic>\n isolate, native to citrus, and demonstrated their ability to inhibit\n Liberibacter<\/jats:italic>\n spp. and reduce\n C<\/jats:italic>\n Las populations in citrus tissue. This study highlights how microbial discovery can lead to the identification of antimicrobial compounds with potential applications in plant disease management.\n <\/jats:p>\n <\/jats:sec>\n <\/jats:sec>","DOI":"10.1128\/aem.00869-25","type":"journal-article","created":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T12:52:29Z","timestamp":1753966349000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Amicoumacins produced by the native citrus microbiome isolate\n Bacillus safensis<\/i>\n inhibit the Huanglongbing-associated bacterial pathogen \u201c\n Candidatus<\/i>\n Liberibacter asiaticus\u201d"],"prefix":"10.1128","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7450-8363","authenticated-orcid":true,"given":"Fl\u00e1via C.","family":"Vieira","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03nawhv43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Plant Pathology, University of California","place":["Riverside, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kranthi K.","family":"Mandadi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0034eay46","id-type":"ROR","asserted-by":"publisher"}],"name":"Texas A&M AgriLife Research and Extension Center, Texas A&M University System","place":["Weslaco, USA"]},{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Plant Pathology and Microbiology, Texas A&M University","place":["College Station, USA"]},{"name":"Institute for Advancing Health Through Agriculture, Texas A&M AgriLife","place":["Weslaco, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1788-241X","authenticated-orcid":true,"given":"Manikandan","family":"Ramasamy","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0034eay46","id-type":"ROR","asserted-by":"publisher"}],"name":"Texas A&M AgriLife Research and Extension Center, Texas A&M University System","place":["Weslaco, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6447-3905","authenticated-orcid":true,"given":"Amancio","family":"de Souza","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03nawhv43","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Integrative Genome Biology, University of California","place":["Riverside, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiana","family":"Callahan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02rsjqy82","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Point Loma Nazarene University","place":["San Diego, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corrie","family":"Fyle","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02rsjqy82","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Point Loma Nazarene University","place":["San Diego, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Kamemoto","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02rsjqy82","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Point Loma Nazarene University","place":["San Diego, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda G.","family":"Koontz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02rsjqy82","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Point Loma Nazarene University","place":["San Diego, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Yang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02rsjqy82","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Point Loma Nazarene University","place":["San Diego, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6601-4734","authenticated-orcid":true,"suffix":"III","given":"Robert","family":"Crowley","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of California","place":["Riverside, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4676-8846","authenticated-orcid":false,"given":"Kevin G. M.","family":"Kou","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of California","place":["Riverside, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3472-7593","authenticated-orcid":true,"given":"Katherine N.","family":"Maloney","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02rsjqy82","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Point Loma Nazarene University","place":["San Diego, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4637-6225","authenticated-orcid":true,"given":"M. Caroline","family":"Roper","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03nawhv43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Plant Pathology, University of California","place":["Riverside, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-15308-3_2"},{"key":"e_1_3_4_3_2","unstructured":"USDA. 2025. Citrus: world markets and trade | World production markets and trade report. Available from: https:\/\/www.fas.usda.gov\/data\/citrus-world-markets-and-trade-01302025. Retrieved 21 Apr 2025."},{"key":"e_1_3_4_4_2","first-page":"7","article-title":"Huanglongbing: a destructive, newly-emerging, century-old disease of citrus","volume":"88","author":"Bov\u00e9 JM","year":"2006","unstructured":"Bov\u00e9 JM. 2006. Huanglongbing: a destructive, newly-emerging, century-old disease of citrus. J Plant Path 88:7\u201337.","journal-title":"J Plant Path"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1653\/0015-4040(2004)087[0330:ACPSPA]2.0.CO;2"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.19.01391"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10658-011-9779-1"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/9781119281269.ch7"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-06-19-0198-R"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40858-020-00335-y"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.00933"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-77840-2"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40858-020-00362-9"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-28189-9"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1094\/PDIS-10-22-2336-RE"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTOFR-02-23-0025-R"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/ps.3957"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2019628118"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-62246-x"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11061529"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2024.109232"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-70499-z"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.70013"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-05-17-0175-R"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11081092"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/agriculture12101592"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-09-22-0330-R"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2023.106350"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-0412-1"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02883-19"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-54685-y"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/jam.14506"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10030596"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8071037"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.14348"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-09-16-0330-RVW"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy9080470"},{"key":"e_1_3_4_38_2","first-page":"385","volume-title":"Studies in natural products chemistry","author":"Tyurin AP","year":"2018","unstructured":"Tyurin AP, Efimenko TA, Prokhorenko IA, Rogozhin EA, Malanicheva IA, Zenkova VA, Efremenkova OV, Korshun VA. 2018. Chapter 12 - amicoumacins and related compounds: chemistry and biology, p 385\u2013441. In Studies in natural products chemistry. Elsevier."},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-04-19-0129-R"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/mra.00602-24"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2013.09.006"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep09383"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1811250115"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules21070824"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9040157"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/ja.2007.99"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1351\/PAC-CON-08-09-25"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(02)01320-7"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.3390\/md10020319"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.581802"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-19631-x"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.11.3156-3161.2001"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2014.09.020"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2020.1788554"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.34.611"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep27720"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1021\/np50075a005"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.002899-0"},{"key":"e_1_3_4_59_2","first-page":"130","article-title":"Extraction and identification of antibacterial secondary metabolites from marine Streptomyces sp. VITBRK2","volume":"3","author":"Rajan BM","year":"2014","unstructured":"Rajan BM, Kannabiran K. 2014. Extraction and identification of antibacterial secondary metabolites from marine Streptomyces sp. VITBRK2. Int J Mol Cell Med 3:130\u2013137.","journal-title":"Int J Mol Cell Med"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb1961.46.2659"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2007.09.024"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-010-0485-5"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1021\/jo500273f"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.3891\/acta.chem.scand.04-0283"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1094\/PBIOMES-04-20-0027-R"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1515\/biolog-2015-0062"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.734216"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.3390\/plants13010075"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2024.1451887"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4163"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab301"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad344"},{"key":"e_1_3_4_73_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-023-05311-2"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/5893123"},{"key":"e_1_3_4_75_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-020-0933-6"},{"key":"e_1_3_4_76_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3597"},{"key":"e_1_3_4_77_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-023-01690-2"},{"key":"e_1_3_4_78_2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1777"},{"key":"e_1_3_4_79_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06082-8"},{"key":"e_1_3_4_80_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_3_4_81_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01411-19"},{"key":"e_1_3_4_82_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-41495-5"},{"key":"e_1_3_4_83_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.9b01207"},{"key":"e_1_3_4_84_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep39020"},{"key":"e_1_3_4_85_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031263"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.00869-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.00869-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T13:02:26Z","timestamp":1755694946000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.00869-25"}},"subtitle":[],"editor":[{"given":"Gladys","family":"Alexandre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,8,20]]},"references-count":84,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8,20]]}},"alternative-id":["10.1128\/aem.00869-25"],"URL":"https:\/\/doi.org\/10.1128\/aem.00869-25","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,20]]},"assertion":[{"value":"2025-04-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00869-25"}}