{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:30:44Z","timestamp":1787016644487,"version":"build-2736575974"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2014,7,15]],"date-time":"2014-07-15T00:00:00Z","timestamp":1405382400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2014,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The anaerobic dehalogenation of organohalides is catalyzed by the reductive dehalogenase (RdhA) enzymes produced in phylogenetically diverse bacteria. These enzymes contain a cobamide cofactor at the active site and two iron-sulfur clusters. In this study, the tetrachloroethene (PCE) reductive dehalogenase (PceA) of the Gram-positive\n                    <jats:named-content content-type=\"genus-species\">Desulfitobacterium hafniense<\/jats:named-content>\n                    strain Y51 was produced in a catalytically active form in the nondechlorinating, cobamide-producing bacterium\n                    <jats:named-content content-type=\"genus-species\">Shimwellia blattae<\/jats:named-content>\n                    (ATCC 33430), a Gram-negative gammaproteobacterium. The formation of recombinant catalytically active PceA enzyme was significantly enhanced when its dedicated PceT chaperone was coproduced and when 5,6-dimethylbenzimidazole and hydroxocobalamin were added to the\n                    <jats:named-content content-type=\"genus-species\">S. blattae<\/jats:named-content>\n                    cultures. The experiments were extended to\n                    <jats:named-content content-type=\"genus-species\">D. hafniense<\/jats:named-content>\n                    DCB-2, a reductively dehalogenating bacterium harboring multiple\n                    <jats:italic>rdhA<\/jats:italic>\n                    genes. To elucidate the substrate spectrum of the\n                    <jats:italic>rdhA3<\/jats:italic>\n                    gene product of this organism, the recombinant enzyme was tested for the conversion of different dichlorophenols (DCP) in crude extracts of an RdhA3-producing\n                    <jats:named-content content-type=\"genus-species\">S. blattae<\/jats:named-content>\n                    strain. 3,5-DCP, 2,3-DCP, and 2,4-DCP, but not 2,6-DCP and 3,4-DCP, were reductively dechlorinated by the recombinant RdhA3. In addition, this enzyme dechlorinated PCE to trichloroethene at low rates.\n                  <\/jats:p>","DOI":"10.1128\/aem.00881-14","type":"journal-article","created":{"date-parts":[[2014,5,9]],"date-time":"2014-05-09T22:27:14Z","timestamp":1399674434000},"page":"4313-4322","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Functional Heterologous Production of Reductive Dehalogenases from Desulfitobacterium hafniense Strains"],"prefix":"10.1128","volume":"80","author":[{"given":"Anita","family":"Mac Nelly","sequence":"first","affiliation":[{"name":"Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Kai","sequence":"additional","affiliation":[{"name":"Research Group Mass Spectrometry, Max Planck Institute for Chemical Ecology, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ale\u0161","family":"Svato\u0161","sequence":"additional","affiliation":[{"name":"Research Group Mass Spectrometry, Max Planck Institute for Chemical Ecology, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Diekert","sequence":"additional","affiliation":[{"name":"Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torsten","family":"Schubert","sequence":"additional","affiliation":[{"name":"Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University, Jena, 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