{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:35:39Z","timestamp":1790573739350,"version":"4.1.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2006,7,1]],"date-time":"2006-07-01T00:00:00Z","timestamp":1151712000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We compared the robustness and discriminatory power of the enterobacterial repetitive intergenic consensus (ERIC) and random amplified polymorphic DNA (RAPD) fingerprinting methods for detecting cases of mixed\n            <jats:italic>Helicobacter pylori<\/jats:italic>\n            infection in Peruvian shantytown residents.\n            <jats:italic>H. pylori<\/jats:italic>\n            isolates from 63 participants were cultured, and five single colonies and a pool of additional colonies from each participant were analyzed by ERIC-PCR and by RAPD tests with four 10-nucleotide primers (one primer per reaction). There was 94% agreement between the ERIC and RAPD profiles in classifying sets of isolates as uniform versus closely related but not identical versus probably unrelated, indicating a high kappa statistic of 0.8942. Subtle differences in related ERIC or RAPD patterns likely reflect gene transfer between strains, recombination, and\/or mutation, whereas markedly different patterns reflect infection by unrelated strains. At least half of infected shantytown residents seemed to carry more than one\n            <jats:italic>H. pylori<\/jats:italic>\n            strain, although in 19 of 31 persons, the strains were closely related. Three RAPD tests, each with a different primer, were needed to achieve the sensitivity of one ERIC test. ERIC-PCR constitutes a resource- and time-efficient method for\n            <jats:italic>H. pylori<\/jats:italic>\n            strain differentiation.\n          <\/jats:p>","DOI":"10.1128\/aem.00894-06","type":"journal-article","created":{"date-parts":[[2006,7,7]],"date-time":"2006-07-07T01:51:13Z","timestamp":1152237073000},"page":"4713-4716","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Effectiveness of Enterobacterial Repetitive Intergenic Consensus PCR and Random Amplified Polymorphic DNA Fingerprinting for\n            <i>Helicobacter pylori<\/i>\n            Strain Differentiation"],"prefix":"10.1128","volume":"72","author":[{"given":"S. Alison","family":"Finger","sequence":"first","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Universidad Peruana Cayetano Heredia, Lima, Peru"},{"name":"Washington University School of Medicine, St. Louis, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Billie","family":"Velapatin\u0303o","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Universidad Peruana Cayetano Heredia, Lima, Peru"},{"name":"Asociacio\u0301n Bene\u0301fica PRISMA, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret","family":"Kosek","sequence":"additional","affiliation":[{"name":"Department of International Health, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Livia","family":"Santivan\u0303ez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Universidad Peruana Cayetano Heredia, Lima, Peru"},{"name":"Asociacio\u0301n Bene\u0301fica PRISMA, Lima, Peru"},{"name":"Department of Molecular Microbiology and Genetics, Washington University School of Medicine, St. Louis, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiva","family":"Dailidiene","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Genetics, Washington University School of Medicine, St. Louis, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willi","family":"Quino","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Balqui","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Universidad Peruana Cayetano Heredia, Lima, Peru"},{"name":"Asociacio\u0301n Bene\u0301fica PRISMA, Lima, 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