{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T09:19:27Z","timestamp":1769505567453,"version":"3.49.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Organophosphate compounds, which are widely used as pesticides and chemical warfare agents, are cholinesterase inhibitors. These synthetic compounds are resistant to natural degradation and threaten the environment. We constructed a strain of\n            <jats:italic>Pseudomonas putida<\/jats:italic>\n            that can efficiently degrade a model organophosphate, paraoxon, and use it as a carbon, energy, and phosphorus source. This strain was engineered with the\n            <jats:italic>pnp<\/jats:italic>\n            operon from\n            <jats:italic>Pseudomonas<\/jats:italic>\n            sp. strain ENV2030, which encodes enzymes that transform\n            <jats:italic>p<\/jats:italic>\n            -nitrophenol into \u03b2-ketoadipate, and with a synthetic operon encoding an organophosphate hydrolase (encoded by\n            <jats:italic>opd<\/jats:italic>\n            ) from\n            <jats:italic>Flavobacterium<\/jats:italic>\n            sp. strain ATCC 27551, a phosphodiesterase (encoded by\n            <jats:italic>pde<\/jats:italic>\n            ) from\n            <jats:italic>Delftia acidovorans<\/jats:italic>\n            , and an alkaline phosphatase (encoded by\n            <jats:italic>phoA<\/jats:italic>\n            ) from\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            HN854 under control of a constitutive promoter. The engineered strain can efficiently mineralize up to 1 mM (275 mg\/liter) paraoxon within 48 h, using paraoxon as the sole carbon and phosphorus source and an inoculum optical density at 600 nm of 0.03. Because the organism can utilize paraoxon as a sole carbon, energy, and phosphorus source and because one of the intermediates in the pathway (\n            <jats:italic>p<\/jats:italic>\n            -nitrophenol) is toxic at high concentrations, there is no need for selection pressure to maintain the heterologous pathway.\n          <\/jats:p>","DOI":"10.1128\/aem.00907-06","type":"journal-article","created":{"date-parts":[[2006,10,4]],"date-time":"2006-10-04T17:50:39Z","timestamp":1159984239000},"page":"6699-6706","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Mineralization of Paraoxon and Its Use as a Sole C and P Source by a Rationally Designed Catabolic Pathway in\n            <i>Pseudomonas putida<\/i>"],"prefix":"10.1128","volume":"72","author":[{"given":"Matthew","family":"de la Pen\u0303a Mattozzi","sequence":"first","affiliation":[{"name":"Graduate Group in Microbiology"},{"name":"Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sundiep K.","family":"Tehara","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Hong","sequence":"additional","affiliation":[{"name":"and Department of Molecular and Cell Biology, University of California, Berkeley, California"},{"name":"Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jay D.","family":"Keasling","sequence":"additional","affiliation":[{"name":"Graduate Group in Microbiology"},{"name":"Department of Chemical Engineering"},{"name":"Department of Bioengineering"},{"name":"Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(81)90080-9"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac015685f"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00152998"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3354\/ame010115"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.39.2.463-465.1980"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.36.5.668-672.1978"},{"key":"e_1_3_2_8_2","first-page":"U219","volume":"219","year":"2000","unstructured":"Gilbert, E. 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