{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:02:19Z","timestamp":1791176539119,"version":"4.1.0"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Transcripts of\n            <jats:named-content content-type=\"genus-species\">Leptospira<\/jats:named-content>\n            16S rRNA, FlaB, LigB, LipL21, LipL32, LipL36, LipL41, and OmpL37 were quantified in the blood of susceptible (hamsters) and resistant (mice) animal models of leptospirosis. We first validated adequate reference genes and then evaluated expression patterns\n            <jats:italic>in vivo<\/jats:italic>\n            compared to\n            <jats:italic>in vitro<\/jats:italic>\n            cultures. LipL32 expression was downregulated\n            <jats:italic>in vivo<\/jats:italic>\n            and differentially regulated in resistant and susceptible animals. FlaB expression was also repressed in mice but not in hamsters. In contrast, LigB and OmpL37 were upregulated\n            <jats:italic>in vivo<\/jats:italic>\n            . Thus, we demonstrated that a virulent strain of\n            <jats:named-content content-type=\"genus-species\">Leptospira<\/jats:named-content>\n            differentially adapts its gene expression in the blood of infected animals.\n          <\/jats:p>","DOI":"10.1128\/aem.00911-12","type":"journal-article","created":{"date-parts":[[2012,6,24]],"date-time":"2012-06-24T00:33:51Z","timestamp":1340498031000},"page":"6372-6376","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Differential\n            <i>In Vivo<\/i>\n            Gene Expression of Major Leptospira Proteins in Resistant or Susceptible Animal Models"],"prefix":"10.1128","volume":"78","author":[{"given":"Mariko","family":"Matsui","sequence":"first","affiliation":[{"name":"Institut Pasteur de Nouvelle-Cal\u00e9donie, Institut Pasteur International Network, Bacterial Research Laboratory, Noumea, New 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