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Notably,\n                      <jats:italic>Pt<\/jats:italic>\n                      Lam exhibits proficient transglycosylation capabilities, utilizing various sugar alcohols as acceptors, with lyxose, in particular, yielding exclusively transglycosylated products. Structural analysis of both apo-\n                      <jats:italic>Pt<\/jats:italic>\n                      Lam and its laminarin oligosaccharide-bound complex revealed significant conformational alterations in active residues upon substrate binding. Moreover, pivotal residues involved in substrate recognition were identified, with subsequent mutation assays indicating the contribution of positive subsites in modulating exo-hydrolysis and transglycosidic activities. These results enhance our comprehension of laminarin cycling mechanisms by marine\n                      <jats:italic>Planctomycetota<\/jats:italic>\n                      , while also providing essential enzyme components for laminarin hetero-oligosaccharide synthesis.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        The ubiquitous\n                        <jats:italic>Planctomycetota<\/jats:italic>\n                        , with distinctive physiological traits, exert a significant influence on global carbon and nitrogen fluxes. Their intimate association with algae suggests a propensity for efficient polysaccharide degradation; however, research on glycoside hydrolases derived from\n                        <jats:italic>Planctomycetota<\/jats:italic>\n                        remains scarce. Herein, we unveil the GH16 family laminarinase\n                        <jats:italic>Pt<\/jats:italic>\n                        Lam from deep-sea\n                        <jats:italic>Planctomycetota<\/jats:italic>\n                        , shedding light on its catalytic mechanisms underlying hydrolysis and transglycosylation. Our findings elucidate the enzymatic pathways governing the marine laminarin cycle orchestrated by\n                        <jats:italic>Planctomycetota,<\/jats:italic>\n                        thereby fostering the exploration of novel polysaccharide hydrolases with promising practical implications.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/aem.00942-24","type":"journal-article","created":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T13:00:21Z","timestamp":1726578021000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Molecular insights into the hydrolysis and transglycosylation of a deep-sea\n                    <i>Planctomycetota<\/i>\n                    -derived GH16 family laminarinase"],"prefix":"10.1128","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7045-6554","authenticated-orcid":false,"given":"Wei","family":"Li","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Guangdong Provincial Observation and Research Station for Coastal Upwelling Ecosystem, Chinese Academy of Sciences, Guangzhou, China"},{"name":"University of the Chinese Academy of Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Lin","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Guangdong Provincial Observation and Research Station for Coastal Upwelling Ecosystem, Chinese Academy of Sciences, Guangzhou, 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