{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:46:17Z","timestamp":1791049577318,"version":"4.1.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We introduce a near-real-time optical imaging method that works via the detection of the intrinsic fluorescence of life forms upon excitation by deep-UV (DUV) illumination. A DUV (&lt;250-nm) source enables the detection of microbes in their native state on natural materials, avoiding background autofluorescence and without the need for fluorescent dyes or tags. We demonstrate that DUV-laser-induced native fluorescence can detect bacteria on opaque surfaces at spatial scales ranging from tens of centimeters to micrometers and from communities to single cells. Given exposure times of 100 \u03bcs and low excitation intensities, this technique enables rapid imaging of bacterial communities and cells without irreversible sample alteration or destruction. We also demonstrate the first noninvasive detection of bacteria on\n            <jats:italic>in situ<\/jats:italic>\n            -incubated environmental experimental samples from the deep ocean (Lo'ihi Seamount), showing the use of DUV native fluorescence for\n            <jats:italic>in situ<\/jats:italic>\n            detection in the deep biosphere and other nutrient-limited environments.\n          <\/jats:p>","DOI":"10.1128\/aem.00943-10","type":"journal-article","created":{"date-parts":[[2010,9,4]],"date-time":"2010-09-04T02:34:25Z","timestamp":1283567665000},"page":"7231-7237","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["Label-Free Bacterial Imaging with Deep-UV-Laser-Induced Native Fluorescence"],"prefix":"10.1128","volume":"76","author":[{"given":"Rohit","family":"Bhartia","sequence":"first","affiliation":[{"name":"Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California"},{"name":"Department of Earth Sciences, University of Southern California, Los Angeles, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Everett C.","family":"Salas","sequence":"additional","affiliation":[{"name":"Department of Earth Science, Rice University, Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William F.","family":"Hug","sequence":"additional","affiliation":[{"name":"Photon Systems, Inc., Covina, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ray D.","family":"Reid","sequence":"additional","affiliation":[{"name":"Photon Systems, Inc., Covina, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur L.","family":"Lane","sequence":"additional","affiliation":[{"name":"Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrina J.","family":"Edwards","sequence":"additional","affiliation":[{"name":"Department of Earth Sciences, University of Southern California, Los Angeles, California"},{"name":"Department of Biological Sciences, University of Southern California, Los Angeles, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth H.","family":"Nealson","sequence":"additional","affiliation":[{"name":"Department of Earth Sciences, University of Southern California, Los Angeles, California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"448","year":"1997","unstructured":"Banfield, J. 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