{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:31:59Z","timestamp":1787016719339,"version":"build-2736575974"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2009,9,15]],"date-time":"2009-09-15T00:00:00Z","timestamp":1252972800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2009,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A longitudinal study was conducted to investigate the nature of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    O157:H7 colonization of feedlot cattle over the final 100 to 110 days of finishing. Rectal fecal grab samples were collected from an initial sample population of 788 steers every 20 to 22 days and microbiologically analyzed to detect\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157:H7. The identities of presumptive colonies were confirmed using a multiplex PCR assay that screened for gene fragments unique to\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157:H7 (\n                    <jats:italic>rfbE<\/jats:italic>\n                    and\n                    <jats:italic>fliC<\/jats:italic>\n                    <jats:sub>h7<\/jats:sub>\n                    ) and other key virulence genes (\n                    <jats:italic>eae<\/jats:italic>\n                    ,\n                    <jats:italic>stx<\/jats:italic>\n                    <jats:sub>1<\/jats:sub>\n                    , and\n                    <jats:italic>stx<\/jats:italic>\n                    <jats:sub>2<\/jats:sub>\n                    ). Animals were classified as having persistent shedding (PS), transient shedding (TS), or nonshedding (NS) status if they consecutively shed the same\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157:H7 genotype (based on the multiplex PCR profile), exhibited variable\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157 shedding, or never shed morphologically typical\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157, respectively. Overall, 1.0% and 1.4% of steers were classified as PS and NS animals, respectively. Characterization of 132\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157:H7 isolates from PS and TS animals by pulsed-field gel electrophoresis (PFGE) typing yielded 32 unique PFGE types. One predominant PFGE type accounted for 53% of all isolates characterized and persisted in cattle throughout the study. Isolates belonging to this predominant and persistent PFGE type demonstrated an enhanced (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.0001) ability to adhere to Caco-2 human intestinal epithelial cells compared to isolates belonging to less common PFGE types but exhibited equal virulence expression. Interestingly, the attachment efficacy decreased as the genetic divergence from the predominant and persistent subtype increased. Our data support the hypothesis that certain\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157:H7 strains persist in feedlot cattle, which may be partially explained by an enhanced ability to colonize the intestinal epithelium.\n                  <\/jats:p>","DOI":"10.1128\/aem.00972-09","type":"journal-article","created":{"date-parts":[[2009,7,17]],"date-time":"2009-07-17T21:33:01Z","timestamp":1247866381000},"page":"5927-5937","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["<i>Escherichia coli<\/i>\n                    O157:H7 Strains That Persist in Feedlot Cattle Are Genetically Related and Demonstrate an Enhanced Ability To Adhere to Intestinal Epithelial Cells"],"prefix":"10.1128","volume":"75","author":[{"given":"Brandon A.","family":"Carlson","sequence":"first","affiliation":[{"name":"Center for Meat Safety &amp; Quality, Department of Animal Sciences, Colorado State University, Fort Collins, Colorado 80523-1171"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kendra K.","family":"Nightingale","sequence":"additional","affiliation":[{"name":"Center for Meat Safety &amp; Quality, Department of Animal Sciences, Colorado State University, Fort Collins, Colorado 80523-1171"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary L.","family":"Mason","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523-1619"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Ruby","sequence":"additional","affiliation":[{"name":"JBS Packerland Inc., Green Bay, Wisconsin 54311"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. Travis","family":"Choat","sequence":"additional","affiliation":[{"name":"Elanco Animal Health, Greenfield, Indiana 46140"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy H.","family":"Loneragan","sequence":"additional","affiliation":[{"name":"Feedlot Research Group, West Texas A&amp;M University, Canyon, Texas 79016-0001"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary C.","family":"Smith","sequence":"additional","affiliation":[{"name":"Center for Meat Safety &amp; Quality, Department of Animal Sciences, Colorado State University, Fort Collins, Colorado 80523-1171"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John N.","family":"Sofos","sequence":"additional","affiliation":[{"name":"Center for Meat Safety &amp; Quality, Department of Animal Sciences, Colorado State University, Fort Collins, Colorado 80523-1171"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith E.","family":"Belk","sequence":"additional","affiliation":[{"name":"Center for Meat Safety &amp; Quality, Department of Animal Sciences, Colorado State University, Fort Collins, Colorado 80523-1171"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2004.02465.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-65.2.280"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-65.10.1527"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-66.11.1978"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-68.11.2264"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/175.3.726"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1017\/S095026880100615X"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.1.27-32.1997"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-63.11.1467"},{"key":"e_1_3_2_11_2","unstructured":"One-day (24-28 h) standardized laboratory protocol for molecular subtyping of Escherichia coli O157:H7 by pulsed-field gel electrophoresis (PFGE). 2001"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-69.6.1240"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01742-06"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00235"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.5.2704-2707.2002"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.4.1586-1590.1995"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.5.1842-1848.1997"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-4143-1_16"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2005.66.2023"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.7.2999"},{"key":"e_1_3_2_21_2","unstructured":"Food and Drug Administration United States Department of Health and Human Services. 2001. Bacteriological analytical manual. http:\/\/vm.cfsan.fda.gov\/\u223cebam\/bam-toc.html\n. Accessed 20 September 2006."},{"key":"e_1_3_2_22_2","unstructured":"Food Safety Inspection Service United States Department of Agriculture. 2008. Detection isolation and identification of Escherichia coli O157:H7 from meat products. http:\/\/www.fsis.usda.gov\/PDF\/MLG_5_04.pdf\n. Accessed 13 May 2008."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.4.1619-1627.2001"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02893-06"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.5.2269-2277.2002"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2006-508"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.1999.00938.x"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.3.1045-1050.2005"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2006-421"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-68.10.2224"},{"key":"e_1_3_2_31_2","first-page":"123","volume":"2","year":"2004","unstructured":"Kaper, J. B., J. P. Nataro, and H. L. T. Mobley. 2004. Pathogenic Escherichia coli. Nat. Rev.2:123-140.","journal-title":"Nat. Rev."},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-66.11.1972"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1090-0233(02)00162-4"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.1.377-384.2004"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.1.93-97.2005"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268805004590"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0503776103"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)01267-7"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0505.990502"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0450.2003.00674.x"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.3.1505-1512.2003"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1993.tb06554.x"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004670100660"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198303243081203"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2004.02390.x"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(99)00106-6"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2005.11.066"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.8.4588-4595.2004"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00406-06"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.4.1390-1399.1998"},{"key":"e_1_3_2_51_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(05)73131-3"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-68.1.26"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063870401600114"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.33.9.2233-2239.1995"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-63.12.1630"},{"key":"e_1_3_2_57_2","first-page":"332","volume":"40","year":"1999","unstructured":"Van Donkersgoed, J., T. Graham, and V. Gannon. 1999. The prevalence of verotoxins, Escherichia coli O157:H7, and Salmonella in the feces and rumen of cattle at processing. Can. Vet. J.40:332-338.","journal-title":"Can. Vet. J."},{"key":"e_1_3_2_58_2","first-page":"714","volume":"42","year":"2001","unstructured":"Van Donkersgoed, J., J. Berg, A. Potter, D. Hancock, T. Besser, D. Rice, J. LeJeune, and S. Klashinsky. 2001. Environmental sources and transmission of Escherichia coli O157 in feedlot cattle. Can. Vet. J.42:714-720.","journal-title":"Can. Vet. J."},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-69.12.2824"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00972-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00972-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T12:43:52Z","timestamp":1645620232000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.00972-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,15]]},"references-count":58,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2009,9,15]]}},"alternative-id":["10.1128\/AEM.00972-09"],"URL":"https:\/\/doi.org\/10.1128\/aem.00972-09","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9,15]]},"assertion":[{"value":"2009-04-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-07-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-09-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}