{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:53:28Z","timestamp":1790672008191,"version":"4.1.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"22","license":[{"start":{"date-parts":[[2007,11,15]],"date-time":"2007-11-15T00:00:00Z","timestamp":1195084800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2007,11,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The genus\n            <jats:italic>Brucella<\/jats:italic>\n            includes a number of species that are major animal pathogens worldwide and significant causes of zoonotic infections of humans. Traditional methods of identifying\n            <jats:italic>Brucella<\/jats:italic>\n            to the species level can be time-consuming, can be subjective, and can pose a hazard to laboratory personnel in the absence of suitable biocontainment facilities. Using a robust phylogenetic framework, a number of single-nucleotide polymorphisms (SNPs) that define particular species within the genus were identified. These SNPs were used to develop a multiplex SNP detection assay, based on primer extension technology, that can rapidly and unambiguously identify an isolate as a member of one of the six classical\n            <jats:italic>Brucella<\/jats:italic>\n            species or as a member of the recently identified marine mammal group.\n          <\/jats:p>","DOI":"10.1128\/aem.00976-07","type":"journal-article","created":{"date-parts":[[2007,9,22]],"date-time":"2007-09-22T00:53:43Z","timestamp":1190422423000},"page":"7331-7337","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Multiplex Assay Based on Single-Nucleotide Polymorphisms for Rapid Identification of\n            <i>Brucella<\/i>\n            Isolates at the Species Level"],"prefix":"10.1128","volume":"73","author":[{"given":"Julie C.","family":"Scott","sequence":"first","affiliation":[{"name":"Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark S.","family":"Koylass","sequence":"additional","affiliation":[{"name":"Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Stubberfield","sequence":"additional","affiliation":[{"name":"Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian M.","family":"Whatmore","sequence":"additional","affiliation":[{"name":"Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Techniques for the brucellosis laboratory 1988"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45971-0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.11.2660-2666.1994"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.6.1640-1642.1995"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408410500304082"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.4.1409-1413.2004"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-144-12-3267"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01453-06"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.4.1995-1997.2005"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063879400600408"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.2.759-772.2006"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.138.24.583"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3649-3654.2004"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47330-0"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.8.2715-2726.2005"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.8.4784-4792.2005"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00843-06"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(97)00118-1"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2004.02.005"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00836-06"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64349-0"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-139-12-3265"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70382-6"},{"key":"e_1_3_2_25_2","first-page":"359","volume":"134","year":"1994","unstructured":"Ross, H. 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