{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:39:55Z","timestamp":1789180795150,"version":"build-2803163510"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2006,9,1]],"date-time":"2006-09-01T00:00:00Z","timestamp":1157068800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The dehalorespiring\n            <jats:italic>Desulfitobacterium hafniense<\/jats:italic>\n            strain Y51 efficiently dechlorinates tetrachloroethene (PCE) to\n            <jats:italic>cis<\/jats:italic>\n            -1,2-dichloroethene (\n            <jats:italic>cis<\/jats:italic>\n            -DCE) via trichloroethene by PceA reductive dehalogenase encoded by the\n            <jats:italic>pceA<\/jats:italic>\n            gene. In a previous study, we found that the significant growth inhibition of strain Y51 occurred in the presence of commercial\n            <jats:italic>cis<\/jats:italic>\n            -DCE. In this study, it turned out that the growth inhibition was caused by chloroform (CF) contamination of\n            <jats:italic>cis<\/jats:italic>\n            -DCE. Interestingly, CF did not affect the growth of PCE-nondechlorinating SD (small deletion) and LD (large deletion) variants, where the former fails to transcribe the\n            <jats:italic>pceABC<\/jats:italic>\n            genes caused by a deletion of the promoter and the latter lost the entire\n            <jats:italic>pceABCT<\/jats:italic>\n            gene cluster. Therefore, PCE-nondechlorinating variants, mostly LD variant, became predominant, and dechlorination activity was significantly reduced in the presence of CF. Moreover, such a growth inhibitory effect was also observed in the presence of carbon tetrachloride at 1 \u03bcM, but not carbon dichloride even at 1 mM.\n          <\/jats:p>","DOI":"10.1128\/aem.00979-06","type":"journal-article","created":{"date-parts":[[2006,9,6]],"date-time":"2006-09-06T23:18:42Z","timestamp":1157584722000},"page":"5998-6003","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Effects of Chloromethanes on Growth of and Deletion of the\n            <i>pce<\/i>\n            Gene Cluster in Dehalorespiring\n            <i>Desulfitobacterium hafniense<\/i>\n            Strain Y51"],"prefix":"10.1128","volume":"72","author":[{"given":"Taiki","family":"Futagami","sequence":"first","affiliation":[{"name":"Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takehito","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun-ichi","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masatoshi","family":"Goto","sequence":"additional","affiliation":[{"name":"Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kensuke","family":"Furukawa","sequence":"additional","affiliation":[{"name":"Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.9.3195-3201.1995"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.94.1.171-175.1967"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.16.5332-5339.1992"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02818543"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00249173"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1354(02)00151-3"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-005-0252-z"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-005-0112-9"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.12.5212-5221.1999"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00871642"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1998.tb00377.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/es001285i"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.56.3.482-507.1992"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.28.16515"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.6.2262-2274.2006"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.48.4.840-848.1984"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(00)00316-1"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.58.030603.123600"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.65.1474"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.13.3419-3425.2002"},{"key":"e_1_3_2_22_2","first-page":"241","year":"1987","unstructured":"Wilson, K. 1987. 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