{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:39:50Z","timestamp":1792676390468,"version":"4.5.0"},"reference-count":71,"publisher":"American Society for Microbiology","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2017-70016-26053, 2020-70029-33202, 2021-70029-36056, 2025-70029-44033, 1026705, TEX0-9621, TEX0-7790, AgriLife IVD 114190-96210"],"award-info":[{"award-number":["2017-70016-26053, 2020-70029-33202, 2021-70029-36056, 2025-70029-44033, 1026705, TEX0-9621, TEX0-7790, AgriLife IVD 114190-96210"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006759","name":"California Department of Food and Agriculture","doi-asserted-by":"publisher","award":["24-0001-028-SF"],"award-info":[{"award-number":["24-0001-028-SF"]}],"id":[{"id":"10.13039\/100006759","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009771","name":"American Society of Pharmacognosy","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009771","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"abstract":"ABSTRACT<\/jats:title>\n \n \n \n Huanglongbing (HLB) is a devastating and incurable citrus disease associated with the gram-negative, phloem-limited, and unculturable bacterium \u201c\n Candidatus<\/jats:italic>\n Liberibacter asiaticus\u201d (\n C<\/jats:italic>\n Las), transmitted by the psyllid\n Diaphorina citri<\/jats:italic>\n . Our goal was to identify high-performing microbial candidates from a previously established citrus-associated microbial collection for the management of HLB and associated root pathogens. Candidate selection was based on bioactivity, genomic, and metabolomic profiles. Eighteen\n Bacillus<\/jats:italic>\n isolates were screened for antagonistic activity against\n Liberibacter crescens<\/jats:italic>\n ,\n C<\/jats:italic>\n Las, and citrus-associated\n Phytophthora<\/jats:italic>\n and\n Fusarium<\/jats:italic>\n spp. Phylogenetic analysis grouped the isolates into four species groups:\n Bacillus velezensis<\/jats:italic>\n ,\n B. subtilis<\/jats:italic>\n ,\n B. pumilus<\/jats:italic>\n , and\n B. cereus<\/jats:italic>\n . Among these,\n B. velezensis<\/jats:italic>\n exhibited the strongest and broadest inhibitory activity, followed by\n B. subtilis<\/jats:italic>\n . This enhanced antagonistic potential is likely associated with the greater number and diversity of biosynthetic gene clusters (BGCs) identified. Notably,\n C<\/jats:italic>\n Las-inhibitory isolates included\n B. velezensis<\/jats:italic>\n (CB0021 and CB0909) and\n B. altitudinis<\/jats:italic>\n (CB0086). Bacilysin and surfactin BGCs were conserved across all\n C<\/jats:italic>\n Las-inhibitory isolates, suggesting a potential role in\n C<\/jats:italic>\n Las suppression. Accordingly, surfactins were detected in bioactive fractions of\n B. velezensis<\/jats:italic>\n (CB0027 and CB0909) and\n B. subtilis<\/jats:italic>\n (CB0893), supporting their likely contribution to bioactivity and inhibiting\n L. crescens<\/jats:italic>\n growth\n in vitro<\/jats:italic>\n , highlighting their potential as candidate compounds for\n C<\/jats:italic>\n Las management. Overall, this study underscores the importance of microbial discovery, screening, and characterization as an effective strategy for identifying sustainable solutions to manage HLB.\n <\/jats:p>\n \n IMPORTANCE<\/jats:title>\n \n Huanglongbing (HLB) is a severe disease of citrus that has altered the citrus landscape worldwide and currently has no cure. It is caused by the bacterium \u201c\n Candidatus<\/jats:italic>\n Liberibacter asiaticus\u201d (\n C<\/jats:italic>\n Las) and transmitted by the psyllid\n Diaphorina citri<\/jats:italic>\n . Currently, the most effective control strategy for this disease relies on trunk injection of antibiotics oxytetracycline and streptomycin, and there is a need to find alternative solutions to complement or replace chemical control. Here, we aimed to select high-performing candidates from our previously established citrus-associated microbial collection to manage HLB and root-associated pathogens. We screened 18\n Bacillus<\/jats:italic>\n isolates for antagonistic activity against\n Liberibacter crescens<\/jats:italic>\n ,\n C<\/jats:italic>\n Las, and citrus-associated\n Phytophthora<\/jats:italic>\n and\n Fusarium<\/jats:italic>\n spp., and we generated their genomic and metabolomic profiles. Our research centers on selecting microbial candidates for their bioactivity and antimicrobial metabolite production, aiming to develop effective plant-protective bioinoculants for mitigation of HLB.\n <\/jats:p>\n <\/jats:sec>\n <\/jats:sec>","DOI":"10.1128\/aem.00981-26","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:00:47Z","timestamp":1792674047000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Chemical diversity analysis identifies inhibitory compounds in the secretomes of citrus-associated\n Bacillus<\/i>\n spp. to the Huanglongbing pathogen, \u201c\n Candidatus<\/i>\n Liberibacter asiaticus,\u201d and citrus root pathogens"],"prefix":"10.1128","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7450-8363","authenticated-orcid":true,"given":"Flavia Campos","family":"Vieira","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03nawhv43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Plant Pathology, University of California","place":["Riverside, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1788-241X","authenticated-orcid":true,"given":"Manikandan","family":"Ramasamy","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0034eay46","id-type":"ROR","asserted-by":"publisher"}],"name":"Texas A&M AgriLife Research & Extension Center, Texas A&M University System","place":["Weslaco, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kranthi","family":"Mandadi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0034eay46","id-type":"ROR","asserted-by":"publisher"}],"name":"Texas A&M AgriLife Research & Extension Center, Texas A&M University System","place":["Weslaco, USA"]},{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Plant Pathology and Microbiology, Texas A&M University","place":["College Station, USA"]},{"name":"Institute for Advancing Health Through Agriculture, Texas A&M AgriLife","place":["College Station, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Bossi","family":"Esteves","sequence":"additional","affiliation":[{"name":"Citrus Research Center \u201cSylvio Moreira\u201d\/Agronomic Institute \u2013 IAC","place":["Cordeir\u00f3polis, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6447-3905","authenticated-orcid":true,"given":"Amancio","family":"de Souza","sequence":"additional","affiliation":[{"name":"Metabolomics Core Facility, University of California","place":["Riverside, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Macie","family":"Allen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02rsjqy82","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Point Loma Nazarene University","place":["San Diego, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Ruiz Rivera","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02rsjqy82","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Point Loma Nazarene University","place":["San Diego, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Yang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02rsjqy82","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Point Loma Nazarene University","place":["San Diego, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3472-7593","authenticated-orcid":true,"given":"Katherine N.","family":"Maloney","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02rsjqy82","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Point Loma Nazarene University","place":["San Diego, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4637-6225","authenticated-orcid":true,"given":"M. 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