{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T23:33:33Z","timestamp":1698708813789},"reference-count":64,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Beta-glucuronidase-negative, sorbitol-nonfermenting isolates of Shiga toxin-producing\n            <jats:italic>Escherichia coli<\/jats:italic>\n            O157 comprise part of a clone complex of related enterohemorrhagic\n            <jats:italic>E. coli<\/jats:italic>\n            isolates. High-resolution genotyping shows that the O157 populations have diverged into two different lineages that appear to have different ecologies. To identify genomic regions unique to the most common human-associated genotype, suppression subtractive hybridization was used to identify DNA sequences present in two clinical strains representing the human lineage I O157:H7 strains but absent from two bovine-derived lineage II strains. PCR assays were then used to test for the presence of these regions in 10 lineage I strains and 20 lineage II strains. Twelve conserved regions of genomic difference for lineage I (CRD\n            <jats:sub>I<\/jats:sub>\n            ) were identified that were each present in at least seven of the lineage I strains but absent in most of the lineage II strains tested. The boundaries of the lineage I conserved regions were further delimited by PCR. Eleven of these CRD\n            <jats:sub>I<\/jats:sub>\n            were associated with\n            <jats:italic>E. coli<\/jats:italic>\n            Sakai S-loops 14, 16, 69, 72, 78, 82, 83, 91 to 93, 153, and 286, and the final CRD\n            <jats:sub>I<\/jats:sub>\n            was located on the pO157 virulence plasmid. Several potential virulence factors were identified within these regions, including a putative hemolysin-activating protein, an iron transport system, and several possible regulatory genes. Cluster analysis based on lineage I conserved regions showed that the presence\/absence of these regions was congruent with the inferred phylogeny of the strains.\n          <\/jats:p>","DOI":"10.1128\/aem.00982-06","type":"journal-article","created":{"date-parts":[[2006,12,26]],"date-time":"2006-12-26T18:42:45Z","timestamp":1167158565000},"page":"22-31","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Identification of\n            <i>Escherichia coli<\/i>\n            O157:H7 Genomic Regions Conserved in Strains with a Genotype Associated with Human Infection"],"prefix":"10.1128","volume":"73","author":[{"given":"Marina","family":"Steele","sequence":"first","affiliation":[{"name":"Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario, Canada N1G 3W4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim","family":"Ziebell","sequence":"additional","affiliation":[{"name":"Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario, Canada N1G 3W4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongxiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Lethbridge, Alberta, Canada T1J 3Z4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Benson","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, University of Nebraska, Lincoln, Nebraska 68583-0919"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulina","family":"Konczy","sequence":"additional","affiliation":[{"name":"Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario, Canada N1G 3W4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger","family":"Johnson","sequence":"additional","affiliation":[{"name":"Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario, Canada N1G 3W4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor","family":"Gannon","sequence":"additional","affiliation":[{"name":"Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Lethbridge, Alberta, Canada T1J 3Z4"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","first-page":"2012","year":"1996","unstructured":"Bacheller, S., E. 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