{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T22:47:44Z","timestamp":1785192464154,"version":"3.55.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2023,11,29]],"date-time":"2023-11-29T00:00:00Z","timestamp":1701216000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,11,29]],"date-time":"2023-11-29T00:00:00Z","timestamp":1701216000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["AI137412"],"award-info":[{"award-number":["AI137412"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["AI144187"],"award-info":[{"award-number":["AI144187"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Texas EcoLabs"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2023,11,29]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Ornithodoros turicata<\/jats:italic>\n            is a vector of relapsing fever spirochetes in North America and transmits\n            <jats:italic>Borrelia turicatae<\/jats:italic>\n            to a variety of vertebrate hosts. The remarkably long lifespan of\n            <jats:italic>O. turicata<\/jats:italic>\n            and its ability to maintain spirochetes horizontally (between life stages) and vertically (to progeny) promote the perpetuation of\n            <jats:italic>B. turicatae<\/jats:italic>\n            in nature. Nevertheless, the reproductive biology of\n            <jats:italic>O. turicata<\/jats:italic>\n            is poorly understood. In this report, we collected ticks from a park within a neighborhood of Austin, TX. They were reared to adulthood, and male ticks were individually housed with females. We observed autogenous reproduction, which is the ability to produce eggs without the need for a blood meal, and further investigated vertical transmission of\n            <jats:italic>B. turicatae<\/jats:italic>\n            by quantifying filial infection rates in a cohort of progeny ticks. These results indicate that\n            <jats:italic>O. turicata<\/jats:italic>\n            transovarially transmits\n            <jats:italic>B. turicatae<\/jats:italic>\n            during autogenous reproduction and further signify the tick as a natural reservoir of the spirochetes.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Previous research has implicated\n              <jats:italic>Ornithodoros<\/jats:italic>\n              ticks, including\n              <jats:italic>Ornithodoros turicata<\/jats:italic>\n              , as long-term reservoirs of relapsing fever (RF) spirochetes. Considering the tick\u2019s long lifespan and their efficiency in maintaining and transferring spirochetes within the population, the infection could persist in a given enzootic focus for decades. However, little is known about the relative importance of horizontal and vertical transmission routes in the persistence and evolution of RF\n              <jats:italic>Borrelia<\/jats:italic>\n              . Our observations on the reproductive biology of\n              <jats:italic>O. turicata<\/jats:italic>\n              in the absence of vertebrate hosts indicate an additional mechanism by which\n              <jats:italic>Borrelia turicatae<\/jats:italic>\n              can be maintained in the environment. This work establishes the foundation for studying\n              <jats:italic>O. turicata<\/jats:italic>\n              reproduction and spirochete-vector interactions, which will aid in devising control measures for\n              <jats:italic>Ornithodoros<\/jats:italic>\n              ticks and RF spirochetes.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aem.01032-23","type":"journal-article","created":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T13:03:33Z","timestamp":1698239013000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Autogenous reproduction by\n            <i>Ornithodoros turicata<\/i>\n            (Ixodida: Argasidae) females and vertical transmission of the tick-borne pathogen\n            <i>Borrelia turicatae<\/i>\n            (Spirochaetales:\n            <i>Borreliaceae<\/i>\n            )"],"prefix":"10.1128","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8071-9781","authenticated-orcid":false,"given":"Serhii","family":"Filatov","sequence":"first","affiliation":[{"name":"Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine , Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aparna","family":"Krishnavajhala","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine , Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4802-5779","authenticated-orcid":true,"given":"Job E.","family":"Lopez","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine , Houston, Texas, USA"},{"name":"Department of Molecular Virology and Microbiology, Baylor College of Medicine , Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci3030016"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2411.172033"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2710.210189"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0009868"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.2307\/4582740"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1934.s1-14.479"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.2307\/4584474"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.2307\/3278790"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/28.1.49"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2019.05.014"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.2307\/3281876"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-024937-7.50014-7"},{"key":"e_1_3_4_14_2","volume-title":"Blood-sucking ticks (Ixodoidea)-vectors of diseases of man and animals","author":"Balashov YS.","year":"1967","unstructured":"Balashov YS. 1967. 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