{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:37:01Z","timestamp":1790793421712,"version":"4.1.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2014,10,15]],"date-time":"2014-10-15T00:00:00Z","timestamp":1413331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2014,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Vibrio harveyi<\/jats:named-content>\n            is a marine bacterial pathogen responsible for episodic epidemics generally associated with massive mortalities in many marine organisms, including the European abalone\n            <jats:named-content content-type=\"genus-species\">Haliotis tuberculata<\/jats:named-content>\n            . The aim of this study was to identify the portal of entry and the dynamics of infection of\n            <jats:named-content content-type=\"genus-species\">V. harveyi<\/jats:named-content>\n            in the European abalone. The results indicate that the duration of contact between\n            <jats:named-content content-type=\"genus-species\">V. harveyi<\/jats:named-content>\n            and the European abalone influences the mortality rate and precocity. Immediately after contact, the epithelial and mucosal area situated between the gills and the hypobranchial gland was colonized by\n            <jats:named-content content-type=\"genus-species\">V. harveyi<\/jats:named-content>\n            . Real-time PCR analyses and culture quantification of a green fluorescent protein-tagged strain of\n            <jats:named-content content-type=\"genus-species\">V. harveyi<\/jats:named-content>\n            in abalone tissues revealed a high density of bacteria adhering to and then penetrating the whole gill-hypobranchial gland tissue after 1 h of contact.\n            <jats:named-content content-type=\"genus-species\">V. harveyi<\/jats:named-content>\n            was also detected in the hemolymph of a significant number of European abalones after 3 h of contact. In conclusion, this article shows that a TaqMan real-time PCR assay is a powerful and useful technique for the detection of a marine pathogen such as\n            <jats:named-content content-type=\"genus-species\">V. harveyi<\/jats:named-content>\n            in mollusk tissue and for the study of its infection dynamics. Thus, we have revealed that the adhesion and then the penetration of\n            <jats:named-content content-type=\"genus-species\">V. harveyi<\/jats:named-content>\n            in European abalone organs begin in the first hours of contact. We also hypothesize that the portal of entry of\n            <jats:named-content content-type=\"genus-species\">V. harveyi<\/jats:named-content>\n            in the European abalone is the area situated between the gills and the hypobranchial gland.\n          <\/jats:p>","DOI":"10.1128\/aem.01036-14","type":"journal-article","created":{"date-parts":[[2014,8,9]],"date-time":"2014-08-09T04:55:20Z","timestamp":1407560120000},"page":"6328-6333","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Vibrio harveyi Adheres to and Penetrates Tissues of the European Abalone Haliotis tuberculata within the First Hours of Contact"],"prefix":"10.1128","volume":"80","author":[{"given":"Marion","family":"Cardinaud","sequence":"first","affiliation":[{"name":"UMR 6539-Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Europ\u00e9en de la Mer (IUEM), Universit\u00e9 de Bretagne Occidentale (UBO), CNRS, IRD, Ifremer, Technop\u00f4le Brest Iroise, Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna\u00efck","family":"Barbou","sequence":"additional","affiliation":[{"name":"UMR 6539-Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Europ\u00e9en de la Mer (IUEM), Universit\u00e9 de Bretagne Occidentale (UBO), CNRS, IRD, Ifremer, Technop\u00f4le Brest Iroise, Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carole","family":"Capitaine","sequence":"additional","affiliation":[{"name":"UMR 6539-Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Europ\u00e9en de la Mer (IUEM), Universit\u00e9 de Bretagne Occidentale (UBO), CNRS, IRD, Ifremer, Technop\u00f4le Brest Iroise, Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adeline","family":"Bidault","sequence":"additional","affiliation":[{"name":"UMR 6539-Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Europ\u00e9en de la Mer (IUEM), Universit\u00e9 de Bretagne Occidentale (UBO), CNRS, IRD, Ifremer, Technop\u00f4le Brest Iroise, Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine Marie","family":"Dujon","sequence":"additional","affiliation":[{"name":"UMR 6539-Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Europ\u00e9en de la Mer (IUEM), Universit\u00e9 de Bretagne Occidentale (UBO), CNRS, IRD, Ifremer, Technop\u00f4le Brest Iroise, Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario","family":"Moraga","sequence":"additional","affiliation":[{"name":"UMR 6539-Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Europ\u00e9en de la Mer (IUEM), Universit\u00e9 de Bretagne Occidentale (UBO), CNRS, IRD, Ifremer, Technop\u00f4le Brest Iroise, Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Paillard","sequence":"additional","affiliation":[{"name":"UMR 6539-Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Europ\u00e9en de la Mer (IUEM), Universit\u00e9 de Bretagne Occidentale (UBO), CNRS, IRD, Ifremer, Technop\u00f4le Brest Iroise, Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2006.01989.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.03.015"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3354\/dao050035"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2486.2008.01764.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2008.01367.x"},{"key":"e_1_3_3_7_2","volume-title":"Mims' pathogenesis of infectious disease","author":"Mims C","year":"2002","unstructured":"MimsCNashAStephenJFitzgeraldR. 2002. 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