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Information on the\n            <jats:italic>in situ<\/jats:italic>\n            distribution and expression of pathogens responsible for this risk is to date lacking, largely because of technical bottlenecks in detecting signals from minority bacterial populations within a complex microbial and physicochemical ecosystem. We simulated the contamination of a real high-risk cheese with a natural food isolate of\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            , an enterotoxin-producing pathogen responsible for food poisoning. To overcome the problem of a detection limit in a solid matrix, we chose to work with a fluorescent reporter (superfolder green fluorescent protein) that would allow spatiotemporal monitoring of\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            populations and targeted gene expression. The combination of complementary techniques revealed that\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            localizes preferentially on the cheese surface during ripening. Immunochemistry and confocal laser scanning microscopy enabled us to visualize, in a single image, dairy bacteria and pathogen populations, virulence gene expression, and the toxin produced. This procedure is readily applicable to other genes of interest, other bacteria, and different types of food matrices.\n          <\/jats:p>","DOI":"10.1128\/aem.01042-14","type":"journal-article","created":{"date-parts":[[2014,6,16]],"date-time":"2014-06-16T12:42:14Z","timestamp":1402922534000},"page":"5106-5115","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Following Pathogen Development and Gene Expression in a Food Ecosystem: the Case of a Staphylococcus aureus Isolate in Cheese"],"prefix":"10.1128","volume":"80","author":[{"given":"Isabelle","family":"Fleurot","sequence":"first","affiliation":[{"name":"INRA, UMR 1319 Micalis, AgroParisTech, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Aigle","sequence":"additional","affiliation":[{"name":"INRA, UMR 1319 Micalis, AgroParisTech, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renaud","family":"Fleurot","sequence":"additional","affiliation":[{"name":"INRA, UMR 1198 BDR, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Darrigo","sequence":"additional","affiliation":[{"name":"INRA, UMR 1319 Micalis, AgroParisTech, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques-Antoine","family":"Hennekinne","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Est, Anses, Laboratoire de s\u00e9curit\u00e9 des aliments de Maisons-Alfort, Unit\u00e9 SBCL, Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Gruss","sequence":"additional","affiliation":[{"name":"INRA, UMR 1319 Micalis, AgroParisTech, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elise","family":"Borez\u00e9e-Durant","sequence":"additional","affiliation":[{"name":"INRA, UMR 1319 Micalis, AgroParisTech, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agn\u00e8s","family":"Delacroix-Buchet","sequence":"additional","affiliation":[{"name":"INRA, UMR 1319 Micalis, AgroParisTech, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1701.P21101"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X.JFP-11-058"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2014.3547"},{"key":"e_1_3_2_5_2","first-page":"463","volume-title":"Foodborne bacterial pathogens","author":"Bergdoll MS","year":"1989","unstructured":"BergdollMS. 1989. 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