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The identification of enzymes that participate in the fermentation of phloroglucinol to acetate and butyrate in\n                      <jats:italic>Clostridia<\/jats:italic>\n                      was recently reported. In this study, we present the discovery and characterization of a novel metabolic pathway for phloroglucinol degradation in the bacterium\n                      <jats:italic>Collinsella<\/jats:italic>\n                      sp. zg1085, from marmot respiratory tract. In both the\n                      <jats:italic>Clostridial<\/jats:italic>\n                      and\n                      <jats:italic>Collinsella<\/jats:italic>\n                      pathways, phloroglucinol is first reduced to dihydrophoroglucinol by the NADPH-dependent phloroglucinol reductase (PGR), followed by ring opening to form (\n                      <jats:italic>S<\/jats:italic>\n                      )-3-hydroxy-5-oxohexanoate by a Mn\n                      <jats:sup>2+<\/jats:sup>\n                      -dependent dihydrophloroglucinol cyclohydrolase (DPGC). In the\n                      <jats:italic>Collinsella<\/jats:italic>\n                      pathway, (\n                      <jats:italic>S<\/jats:italic>\n                      )-3-hydroxy-5-oxohexanoate is then cleaved to form malonate semialdehyde and acetone by a newly identified aldolase (HOHA). Finally, a NADP\n                      <jats:sup>+<\/jats:sup>\n                      -dependent malonate-semialdehyde dehydrogenase converts malonate semialdehyde to CO\n                      <jats:sub>2<\/jats:sub>\n                      and acetyl-CoA, an intermediate in carbon and energy metabolism. Recombinant expression of the\n                      <jats:italic>Collinsella<\/jats:italic>\n                      PGR, DPGC, and HOHA in\n                      <jats:italic>E. coli<\/jats:italic>\n                      enabled the conversion of phloroglucinol into acetone, providing support for the proposed pathway. Experiments with\n                      <jats:italic>Olsenella profusa<\/jats:italic>\n                      , another bacterium containing the gene cluster of interest, show that the PGR, DPGC, HOHA, and MSDH are induced by phloroglucinol. Our findings add to the variety of metabolic pathways for the degradation of phloroglucinol, a widely distributed phenolic compound, in the anaerobic microbiome.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Phloroglucinol is an important intermediate in the bacterial degradation of polyphenols, a highly abundant class of plant natural products. Recent research has identified key enzymes of the phloroglucinol degradation pathway in butyrate-producing anaerobic bacteria, which involves cleavage of a linear triketide intermediate by a beta ketoacid cleavage enzyme, requiring acetyl-CoA as a co-substrate. This paper reports a variant of the pathway in the lactic acid bacterium\n                        <jats:italic>Collinsella<\/jats:italic>\n                        sp. zg1085, which involves cleavage of the triketide intermediate by a homolog of deoxyribose-5-phosphate aldolase, highlighting the variety of mechanisms for phloroglucinol degradation by different anaerobic bacterial taxa.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/aem.01047-24","type":"journal-article","created":{"date-parts":[[2024,7,19]],"date-time":"2024-07-19T09:00:22Z","timestamp":1721379622000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["An aldolase-dependent phloroglucinol degradation pathway in\n                    <i>Collinsella<\/i>\n                    sp. zg1085"],"prefix":"10.1128","volume":"90","author":[{"given":"Yating","family":"Li","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Northern Jiangsu People\u2019s Hospital, Clinical Medical School of Yangzhou University, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanqin","family":"Tu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9124-0381","authenticated-orcid":false,"given":"Yifeng","family":"Wei","sequence":"additional","affiliation":[{"name":"Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8867-4859","authenticated-orcid":true,"given":"Yan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5b01173"},{"key":"e_1_3_4_3_2","first-page":"232","volume-title":"Environ. sci. & engg.: toxicology","author":"Abdel-Shafy H","year":"2017","unstructured":"Abdel-Shafy H, Mansour M. 2017. 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