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The aerobic trimethylamine (TMA) oxidation pathway represents the primary route for TMA utilization in marine environments and has been characterized in the model marine Roseobacter group bacterium\n                      <jats:italic toggle=\"yes\">Ruegeria pomeroyi<\/jats:italic>\n                      DSS-3. However, the regulatory mechanism underlying this pathway has remained unclear. Transcriptome analysis revealed coordinated induction of the entire pathway by all intermediate MAs. We identified a master regulator, TmaR, which is essential for the growth of\n                      <jats:italic toggle=\"yes\">R. pomeroyi<\/jats:italic>\n                      DSS-3 on any MA. TmaR functions as an activator of\n                      <jats:italic toggle=\"yes\">tdm<\/jats:italic>\n                      and its downstream enzymatic genes, and as a mild repressor of\n                      <jats:italic toggle=\"yes\">tmm<\/jats:italic>\n                      . It regulates target gene transcription by directly binding to their promoter regions and responds to further catabolite(s) derived from MMA. Another regulator, TmoR, acts as a strong repressor of\n                      <jats:italic toggle=\"yes\">tmm<\/jats:italic>\n                      by binding to its promoter. The\n                      <jats:italic toggle=\"yes\">tmaR<\/jats:italic>\n                      gene is widely distributed among alpha- and gamma-proteobacteria, and in most cases, it is located within MA catabolic gene clusters. Together, these results identify a master regulator of TMA catabolism in globally abundant marine bacteria.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Trimethylamine (TMA) is a key marine nitrogen source; however, its catabolic regulation remains poorly understood. This study identifies TmaR as the master transcriptional regulator of the TMA catabolic pathway in abundant marine bacteria. TmaR is essential for growth on all methylated amines, directly activating downstream genes\u2019 transcription while repressing the initial\n                        <jats:italic toggle=\"yes\">tmm<\/jats:italic>\n                        gene, and responds to downstream MMA catabolite(s). This coordinated regulation enables efficient nitrogen acquisition from mixed methylamine substrates, a common marine scenario. Homologs of TmaR are widespread among marine Alpha- and Gammaproteobacteria, indicating a conserved regulatory strategy for a globally significant biogeochemical process. Our findings provide crucial molecular insight into ocean nitrogen cycling.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/aem.01058-26","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T13:00:36Z","timestamp":1782478836000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Coordinated regulation of trimethylamine catabolism in abundant marine bacteria"],"prefix":"10.1128","volume":"92","author":[{"given":"Na","family":"Wang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04rdtx186","id-type":"ROR","asserted-by":"publisher"}],"name":"MOE Key Laboratory of Evolution and Marine Biodiversity, State Key Laboratory of Marine Food Processing and Safety Control, Frontiers Science Center for Deep Ocean Multispheres and Earth System & College of Marine Life Sciences, Ocean University of 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