{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T12:34:31Z","timestamp":1789734871428,"version":"4.0.1"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2006,9,1]],"date-time":"2006-09-01T00:00:00Z","timestamp":1157068800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            This study characterizes the transcriptional expression of the reductive dehalogenase (RDase)-encoding\n            <jats:italic>tceA<\/jats:italic>\n            and\n            <jats:italic>vcrA<\/jats:italic>\n            genes and evaluates their applicability as potential biological markers of\n            <jats:italic>Dehalococcoides<\/jats:italic>\n            activity. When\n            <jats:italic>Dehalococcoides ethenogenes<\/jats:italic>\n            195 was provided with trichloroethene (TCE) as the electron acceptor, the expression of the\n            <jats:italic>tceA<\/jats:italic>\n            gene increased by 90-fold relative to that in cells starved of chlorinated ethenes, demonstrating that\n            <jats:italic>tceA<\/jats:italic>\n            gene expression is indicative of the active physiological state of this strain. In a\n            <jats:italic>Dehalococcoides<\/jats:italic>\n            -containing enrichment culture that contains both the\n            <jats:italic>tceA<\/jats:italic>\n            and\n            <jats:italic>vcrA<\/jats:italic>\n            genes, the\n            <jats:italic>tceA<\/jats:italic>\n            gene was up-regulated in response to TCE and\n            <jats:italic>cis<\/jats:italic>\n            -1,2-dichloroethene (cDCE) exposure, while the\n            <jats:italic>vcrA<\/jats:italic>\n            gene was up-regulated in response to TCE, cDCE, and vinyl chloride (VC). When chlorinated ethenes were depleted, the RDase-encoding gene transcripts decayed exponentially, with a half-life between 4.8 and 6.1 h, until they reached a stable background level after 2 days. We found that while gene expression correlated generally to the presence of chlorinated ethenes, there was no apparent direct relationship between RDase-encoding transcript numbers and respective rates of TCE, cDCE, and VC dechlorination activities. However, elevated\n            <jats:italic>tceA<\/jats:italic>\n            and\n            <jats:italic>vcrA<\/jats:italic>\n            expression did correlate with chlorinated-ethene reduction beyond cDCE, suggesting that elevated RDase-encoding transcript numbers could serve as a biomarker for the physiological ability of\n            <jats:italic>Dehalococcoides<\/jats:italic>\n            spp. to dechlorinate beyond cDCE.\n          <\/jats:p>","DOI":"10.1128\/aem.01070-06","type":"journal-article","created":{"date-parts":[[2006,9,6]],"date-time":"2006-09-06T23:18:42Z","timestamp":1157584722000},"page":"6161-6168","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":101,"title":["Reductive Dehalogenase Gene Expression as a Biomarker for Physiological Activity of\n            <i>Dehalococcoides<\/i>\n            spp"],"prefix":"10.1128","volume":"72","author":[{"given":"Patrick K. H.","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of California, Berkeley, California 94720-1170"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of California, Berkeley, California 94720-1170"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor F.","family":"Holmes","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of California, Berkeley, California 94720-1170"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhong","family":"He","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of California, Berkeley, California 94720-1170"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Alvarez-Cohen","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of California, Berkeley, California 94720-1170"},{"name":"Earth Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd., Berkeley, California 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/35046063"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.3.1372-1376.2003"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01237"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.9.5183-5189.2004"},{"key":"e_1_3_2_6_2","unstructured":"Committee on Ground Water Cleanup Alternatives National Research Council. 1994. 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