{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T17:57:45Z","timestamp":1790531865144,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2012,9,15]],"date-time":"2012-09-15T00:00:00Z","timestamp":1347667200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Mannheimia haemolytica<\/jats:named-content>,<jats:named-content content-type=\"genus-species\">Pasteurella multocida<\/jats:named-content>, and<jats:named-content content-type=\"genus-species\">Bibersteinia trehalosi<\/jats:named-content>have been identified in the lungs of pneumonic bighorn sheep (BHS;<jats:named-content content-type=\"genus-species\">Ovis canadensis<\/jats:named-content>). Of these pathogens,<jats:named-content content-type=\"genus-species\">M. haemolytica<\/jats:named-content>has been shown to consistently cause fatal pneumonia in BHS under experimental conditions. However,<jats:named-content content-type=\"genus-species\">M. haemolytica<\/jats:named-content>has been isolated by culture less frequently than the other bacteria. We hypothesized that the growth of<jats:named-content content-type=\"genus-species\">M. haemolytica<\/jats:named-content>is inhibited by other bacteria in the lungs of BHS. The objective of this study was to determine whether<jats:named-content content-type=\"genus-species\">P. multocida<\/jats:named-content>inhibits the growth of<jats:named-content content-type=\"genus-species\">M. haemolytica<\/jats:named-content>. Although in monoculture both bacteria exhibited similar growth characteristics, in coculture with<jats:named-content content-type=\"genus-species\">P. multocida<\/jats:named-content>there was a clear inhibition of growth of<jats:named-content content-type=\"genus-species\">M. haemolytica<\/jats:named-content>. The inhibition was detected at mid-log phase and continued through the stationary phase. When cultured in the same medium, the growth of<jats:named-content content-type=\"genus-species\">M. haemolytica<\/jats:named-content>was inhibited when both bacteria were separated by a membrane that allowed contact (pore size, 8.0 \u03bcm) but not when they were separated by a membrane that limited contact (pore size, 0.4 \u03bcm). Lytic bacteriophages or bactericidal compounds could not be detected in the culture supernatant fluid from monocultures of<jats:named-content content-type=\"genus-species\">P. multocida<\/jats:named-content>or from<jats:named-content content-type=\"genus-species\">P. multocida<\/jats:named-content>-<jats:named-content content-type=\"genus-species\">M. haemolytica<\/jats:named-content>cocultures. These results indicate that<jats:named-content content-type=\"genus-species\">P. multocida<\/jats:named-content>inhibits the growth of<jats:named-content content-type=\"genus-species\">M. haemolytica<\/jats:named-content>by a contact- or proximity-dependent mechanism. If the inhibition of growth of<jats:named-content content-type=\"genus-species\">M. haemolytica<\/jats:named-content>by<jats:named-content content-type=\"genus-species\">P. multocida<\/jats:named-content>occurs<jats:italic>in vivo<\/jats:italic>as well, it could explain the inconsistent isolation of<jats:named-content content-type=\"genus-species\">M. haemolytica<\/jats:named-content>from the lungs of pneumonic BHS.<\/jats:p>","DOI":"10.1128\/aem.01119-12","type":"journal-article","created":{"date-parts":[[2012,7,14]],"date-time":"2012-07-14T10:13:06Z","timestamp":1342260786000},"page":"6683-6688","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Proximity-Dependent Inhibition of Growth of Mannheimia haemolytica by Pasteurella multocida"],"prefix":"10.1128","volume":"78","author":[{"given":"Jegarubee","family":"Bavananthasivam","sequence":"first","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohana P.","family":"Dassanayake","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abirami","family":"Kugadas","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudarvili","family":"Shanthalingam","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas R.","family":"Call","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald P.","family":"Knowles","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA"},{"name":"Animal Disease Research Unit, USDA Agricultural Research Service, Washington State University, Pullman, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subramaniam","family":"Srikumaran","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1139\/m84-179"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1115109"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06404.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idairyj.2004.04.003"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01931-07"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1803.111554"},{"key":"e_1_3_2_8_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_9_2","first-page":"3","article-title":"The bighorn sheep in the United States, its past, present, and future","volume":"4","author":"Buechner HK","year":"1960","unstructured":"BuechnerHK. 1960. 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