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In this study, we used both \u201ctop-down\u201d and \u201cbottom-up\u201d strategies to construct two functional consortia (M and Y13) based on non-obligate ODB. Both consortia demonstrated efficient degradation in sediment microcosms, reducing \u03b3-HCH (C\/C\u2080) to 63.0% (M) and 56.1% (Y13). Simultaneously, functional microbial consortia inoculation significantly suppressed the CO\u2082 reduction methanogenesis pathway compared with the non-inoculated group (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05), as evidenced by a marked decline in the CH\u2084\/CO\u2082 ratio in inoculated groups. The well-known degradation gene\n                      <jats:italic toggle=\"yes\">pceA<\/jats:italic>\n                      was enriched in the Y13 group, and the co-localization of\n                      <jats:italic toggle=\"yes\">pceA<\/jats:italic>\n                      -encoding contigs with mobile genetic elements was identified. Notably,\n                      <jats:italic toggle=\"yes\">Enterococcus<\/jats:italic>\n                      was found to have successfully colonized inoculated groups. It has strong environmental evolutionary adaptability and might acquire organochlorine-degrading genes through horizontal gene transfer under pollution stress. The broad applicability of non-obligate ODB (e.g.,\n                      <jats:italic toggle=\"yes\">Enterococcus<\/jats:italic>\n                      ) makes them a promising candidate for future environmental remediation efforts, breeding new avenues of \u201cOne Health\u201d win\u2013win solutions in carbon reduction during pollution remediation for wetlands.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\u03b3\u2011Hexachlorocyclohexane (\u03b3\u2011HCH) is a representative organochlorine pesticide and a well\u2011known persistent organic pollutant that poses significant risks to ecosystems and human health. Microbial anaerobic degradation is a key process in the natural attenuation and engineered cleanup of \u03b3\u2011HCH contamination. The effective application of obligate organohalide-respiring bacteria often requires precise management. In parallel, newly discovered non\u2011obligate organohalide-respiring bacteria capable of degrading \u03b3\u2011HCH have emerged as promising alternatives, yet their performance and ecological interactions in realistic sediment systems remain poorly understood. This study examines the degradation mechanisms and microbial ecology of non-obligate organohalide-respiring bacterial functional consortia in complex media. The findings provide critical insights for developing effective bioaugmentation strategies for lindane\u2011contaminated coastal wetlands and may offer a useful framework for managing other recalcitrant halogenated pollutants in similar environments.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aem.01133-26","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T13:00:38Z","timestamp":1787922038000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Top-down\/bottom-up consortia achieve robust \u03b3-HCH degradation and reduced methanogenic contribution in wetlands"],"prefix":"10.1128","volume":"92","author":[{"given":"Meng","family":"Liu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00a2xv884","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Soil Pollution Control and Safety, College of Environmental and Resource 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