{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:22:55Z","timestamp":1791112975856,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2007,11,1]],"date-time":"2007-11-01T00:00:00Z","timestamp":1193875200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The growing importance of mass spectrometry for the identification and characterization of bacterial protein toxins is a consequence of the improved sensitivity and specificity of mass spectrometry-based techniques, especially when these techniques are combined with affinity methods. Here we describe a novel method based on the use of immunoaffinity capture and matrix-assisted laser desorption ionization-time of flight mass spectrometry for selective purification and detection of staphylococcal enterotoxin B (SEB). SEB is a potent bacterial protein toxin responsible for food poisoning, as well as a potential biological warfare agent. Unambiguous detection of SEB at low-nanogram levels in complex matrices is thus an important objective. In this work, an affinity molecular probe was prepared by immobilizing anti-SEB antibody on the surface of\n            <jats:italic>para<\/jats:italic>\n            -toluene-sulfonyl-functionalized monodisperse magnetic particles and used to selectively isolate SEB. Immobilization and affinity capture procedures were optimized to maximize the density of anti-SEB immunoglobulin G and the amount of captured SEB, respectively, on the surface of magnetic beads. SEB could be detected directly \u201con beads\u201d by placing the molecular probe on the matrix-assisted laser desorption ionization target plate or, alternatively, \u201coff beads\u201d after its acidic elution. Application of this method to complex biological matrices was demonstrated by selective detection of SEB present in different matrices, such as cultivation media of S\n            <jats:italic>taphylococcus aureus<\/jats:italic>\n            strains and raw milk samples.\n          <\/jats:p>","DOI":"10.1128\/aem.01136-07","type":"journal-article","created":{"date-parts":[[2007,9,8]],"date-time":"2007-09-08T00:48:25Z","timestamp":1189212505000},"page":"6945-6952","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":54,"title":["Coupling Immunomagnetic Separation on Magnetic Beads with Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Detection of Staphylococcal Enterotoxin B"],"prefix":"10.1128","volume":"73","author":[{"given":"Gitta","family":"Schlosser","sequence":"first","affiliation":[{"name":"Proteomic and Biomolecular Mass Spectrometry Center (CeSMa-ProBio), Institute of Food Science and Technology, C.N.R., Avellino, Italy"},{"name":"Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Department of Organic Chemistry, E\u00f6tv\u00f6s L. University, Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Ka\u010der","sequence":"additional","affiliation":[{"name":"Proteomic and Biomolecular Mass Spectrometry Center (CeSMa-ProBio), Institute of Food Science and Technology, C.N.R., Avellino, Italy"},{"name":"Department of Organic Technology, Institute of Chemical Technology, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marek","family":"Kuzma","sequence":"additional","affiliation":[{"name":"Proteomic and Biomolecular Mass Spectrometry Center (CeSMa-ProBio), Institute of Food Science and Technology, C.N.R., Avellino, Italy"},{"name":"Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zolt\u00e1n","family":"Szil\u00e1gyi","sequence":"additional","affiliation":[{"name":"Proteomic and Biomolecular Mass Spectrometry Center (CeSMa-ProBio), Institute of Food Science and Technology, C.N.R., Avellino, Italy"},{"name":"Research Institute for Viticulture and Enology, K\u0151lyuktet\u0151, H-3300 Eger, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alida","family":"Sorrentino","sequence":"additional","affiliation":[{"name":"Proteomic and Biomolecular Mass Spectrometry Center (CeSMa-ProBio), Institute of Food Science and Technology, C.N.R., Avellino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Manzo","sequence":"additional","affiliation":[{"name":"Proteomic and Biomolecular Mass Spectrometry Center (CeSMa-ProBio), Institute of Food Science and Technology, C.N.R., Avellino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Pizzano","sequence":"additional","affiliation":[{"name":"Proteomic and Biomolecular Mass Spectrometry Center (CeSMa-ProBio), Institute of Food Science and Technology, C.N.R., Avellino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Livia","family":"Malorni","sequence":"additional","affiliation":[{"name":"Proteomic and Biomolecular Mass Spectrometry Center (CeSMa-ProBio), Institute of Food Science and Technology, C.N.R., Avellino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriella","family":"Pocsfalvi","sequence":"additional","affiliation":[{"name":"Proteomic and Biomolecular Mass Spectrometry Center (CeSMa-ProBio), Institute of Food Science and Technology, C.N.R., Avellino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.mcp.2004.06.003","volume":"18","year":"2004","unstructured":"Alefantisa, T., P. Grewala, J. Ashtona, A. S. Khanb, J. J. Valdesb, and V. G. D. Vecchio. 2004. A rapid and sensitive magnetic bead-based immunoassay for the detection of staphylococcal enterotoxin B for high-throughput screening. Mol. Cell. Probes18:379-382.","journal-title":"Mol. Cell. Probes"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(00)00439-6"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1110.041279"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-68.6.1264"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200206)2:6<740::AID-PROT740>3.0.CO;2-M"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00773-06"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac050485f"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0167-7012(01)00279-2","volume":"46","year":"2001","unstructured":"Boyle, M. D. P., T. G. 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