{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:57:06Z","timestamp":1790837826755,"version":"4.1.0"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T00:00:00Z","timestamp":1285891200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Lactobacilli are autochthonous residents in the chicken gastrointestinal tract, where they may potentially be used as probiotics, competitive exclusion agents, or delivery vehicles. The aim of this study was to use an\n            <jats:italic>in vivo<\/jats:italic>\n            model to investigate the effect of diet and competing lactic acid bacteria on the colonization of inoculated\n            <jats:italic>Lactobacillus<\/jats:italic>\n            strains, with the goal of identifying strains which can consistently colonize or persist for an extended period of several weeks. Chicken-derived\n            <jats:italic>Lactobacillus<\/jats:italic>\n            strains were genetically marked with rifampin resistance and administered on day 0 to chickens fed either a normal commercial diet or a specially formulated high-protein diet. Chickens fed the high-protein diet were also coinoculated with two different mixes of additional lactic acid bacteria. Enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) was used to identify rifampin-resistant isolates recovered from chickens. Three strains, belonging to the species\n            <jats:italic>Lactobacillus agilis<\/jats:italic>\n            ,\n            <jats:italic>Lactobacillus crispatus<\/jats:italic>\n            , and\n            <jats:italic>Lactobacillus vaginalis<\/jats:italic>\n            , were commonly reisolated from the chickens on both diets at days 21 and 42. The ability of these strains to persist was confirmed in a second chicken trial. All three strains persisted throughout the production period in the chickens fed a commercial diet, while only the\n            <jats:italic>L. agilis<\/jats:italic>\n            and\n            <jats:italic>L. vaginalis<\/jats:italic>\n            strains persisted in the chickens fed the high-protein diet. In both\n            <jats:italic>in vivo<\/jats:italic>\n            trials, competing lactic acid bacteria modified representation of the strains recovered, with all three stains capable of competing in the presence of one or both mixes of coinoculated strains. The\n            <jats:italic>in vivo<\/jats:italic>\n            model successfully identified three persistent strains that will be characterized further.\n          <\/jats:p>","DOI":"10.1128\/aem.01137-10","type":"journal-article","created":{"date-parts":[[2010,8,7]],"date-time":"2010-08-07T04:00:31Z","timestamp":1281153631000},"page":"6494-6503","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["<i>Lactobacillus<\/i>\n            Strain Ecology and Persistence within Broiler Chickens Fed Different Diets: Identification of Persistent Strains"],"prefix":"10.1128","volume":"76","author":[{"given":"David P.","family":"Stephenson","sequence":"first","affiliation":[{"name":"Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Moore","sequence":"additional","affiliation":[{"name":"CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria 3220, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwen E.","family":"Allison","sequence":"additional","affiliation":[{"name":"Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia"},{"name":"ANU Medical School, The Australian National University, Canberra, ACT 0200, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01128-07"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/83.7.1093"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1079\/WPS20040017"},{"key":"e_1_3_2_5_2","first-page":"69","year":"2004","unstructured":"Apajalahti, J. 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