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In a previous study, we established the essential role of PGI in cell wall biosynthesis in the opportunistic human fungal pathogen\n              <jats:italic>Aspergillus fumigatus<\/jats:italic>\n              , highlighting its potential as a therapeutic target. In this study, we conducted transcriptomic analysis and discovered that the \u0394\n              <jats:italic>pgi<\/jats:italic>\n              mutant exhibited enhanced glycolysis, reduced PPP, and an upregulation of cell wall precursor biosynthesis pathways. Phenotypic analysis revealed defective protein\n              <jats:italic>N<\/jats:italic>\n              -glycosylation in the mutant, notably the absence of glycosylated virulence factors DPP V and catalase 1. Interestingly, the cell wall defects in the mutant were not accompanied by activation of the MpkA-dependent cell wall integrity (CWI) signaling pathway. Instead, nitrate assimilation was activated in the \u0394\n              <jats:italic>pgi<\/jats:italic>\n              mutant, stimulating glutamine synthesis and providing amino donors for chitin precursor biosynthesis. Blocking the nitrate assimilation pathway severely impaired the growth of the \u0394\n              <jats:italic>pgi<\/jats:italic>\n              mutant, highlighting the crucial role of nitrate assimilation in rescuing cell wall defects. This study unveils the connection between nitrogen assimilation and cell wall compensation in\n              <jats:italic>A. fumigatus<\/jats:italic>\n              .\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                is a common and serious human fungal pathogen that causes a variety of diseases. Given the limited availability of antifungal drugs and increasing drug resistance, it is imperative to understand the fungus\u2019 survival mechanisms for effective control of fungal infections. Our previous study highlighted the essential role of\n                <jats:italic>A. fumigatus<\/jats:italic>\n                PGI in maintaining cell wall integrity, phosphate sugar homeostasis, and virulence. The present study further illuminates the involvement of PGI in protein\n                <jats:italic>N<\/jats:italic>\n                -glycosylation. Furthermore, this research reveals that the nitrogen assimilation pathway, rather than the canonical MpkA-dependent CWI pathway, compensates for cell wall deficiencies in the mutant. These findings offer valuable insights into a novel adaptation mechanism of\n                <jats:italic>A. fumigatus<\/jats:italic>\n                to address cell wall defects, which could hold promise for the treatment of infections.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/aem.01138-24","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T13:00:56Z","timestamp":1724072456000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Nitrate assimilation compensates for cell wall biosynthesis in the absence of\n            <i>Aspergillus fumigatus<\/i>\n            phosphoglucose isomerase"],"prefix":"10.1128","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1471-4813","authenticated-orcid":false,"given":"Xiufang","family":"Gong","sequence":"first","affiliation":[{"name":"Institute of Biological Sciences and 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