{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:51:56Z","timestamp":1791298316634,"version":"4.1.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2007,12,15]],"date-time":"2007-12-15T00:00:00Z","timestamp":1197676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2007,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A synthetic pathway was engineered in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            to produce isopropanol by expressing various combinations of genes from\n            <jats:italic>Clostridium acetobutylicum<\/jats:italic>\n            ATCC 824,\n            <jats:italic>E. coli<\/jats:italic>\n            K-12 MG1655,\n            <jats:italic>Clostridium beijerinckii<\/jats:italic>\n            NRRL B593, and\n            <jats:italic>Thermoanaerobacter brockii<\/jats:italic>\n            HTD4. The strain with the combination of\n            <jats:italic>C. acetobutylicum thl<\/jats:italic>\n            (acetyl-coenzyme A [CoA] acetyltransferase),\n            <jats:italic>E. coli atoAD<\/jats:italic>\n            (acetoacetyl-CoA transferase),\n            <jats:italic>C. acetobutylicum adc<\/jats:italic>\n            (acetoacetate decarboxylase), and\n            <jats:italic>C. beijerinckii adh<\/jats:italic>\n            (secondary alcohol dehydrogenase) achieved the highest titer. This strain produced 81.6 mM isopropanol in shake flasks with a yield of 43.5% (mol\/mol) in the production phase. To our knowledge, this work is the first to produce isopropanol in\n            <jats:italic>E. coli<\/jats:italic>\n            , and the titer exceeded that from the native producers.\n          <\/jats:p>","DOI":"10.1128\/aem.01140-07","type":"journal-article","created":{"date-parts":[[2007,10,13]],"date-time":"2007-10-13T00:44:56Z","timestamp":1192236296000},"page":"7814-7818","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":248,"title":["Engineered Synthetic Pathway for Isopropanol Production in\n            <i>Escherichia coli<\/i>"],"prefix":"10.1128","volume":"73","author":[{"given":"T.","family":"Hanai","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531 Boelter Hall, Los Angeles, California 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Atsumi","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531 Boelter Hall, Los Angeles, California 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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