{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:16:41Z","timestamp":1791299801079,"version":"4.1.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"22","license":[{"start":{"date-parts":[[2007,11,15]],"date-time":"2007-11-15T00:00:00Z","timestamp":1195084800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2007,11,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Little is known about bacterial communities that colonize mucosal surfaces in the human gastrointestinal tract, but they are believed to play an important role in host physiology. The objectives of this study were to investigate the compositions of these populations in the distal small bowel and colon. Healthy mucosal tissue from either the terminal ileum (\n            <jats:italic>n<\/jats:italic>\n            = 6) or ascending (\n            <jats:italic>n<\/jats:italic>\n            = 8), transverse (\n            <jats:italic>n<\/jats:italic>\n            = 8), or descending colon (\n            <jats:italic>n<\/jats:italic>\n            = 4) of 26 patients (age, 68.5 \u00b1 1.2 years [mean \u00b1 standard deviation]) undergoing emergency resection of the large bowel was used to study these communities. Mucosa-associated eubacteria were characterized by using PCR-denaturing gradient gel electrophoresis (DGGE), while real-time PCR was employed for quantitative analysis. Mucosal communities were also visualized in situ using confocal laser scanning microscopy. DGGE banding profiles from all the gut regions exhibited at least 45% homology, with five descending colon profiles clustering at ca. 75% concordance. Real-time PCR showed that mucosal bacterial population densities were highest in the terminal ileum and that there were no significant differences in overall bacterial numbers in different parts of the colon. Bifidobacterial numbers were significantly higher in the large bowel than in the terminal ileum (\n            <jats:italic>P<\/jats:italic>\n            = 0.006), whereas lactobacilli were more prominent in the distal large intestine (\n            <jats:italic>P<\/jats:italic>\n            = 0.019).\n            <jats:italic>Eubacterium rectale<\/jats:italic>\n            (\n            <jats:italic>P<\/jats:italic>\n            = 0.0004) and\n            <jats:italic>Faecalibacterium prausnitzii<\/jats:italic>\n            (\n            <jats:italic>P<\/jats:italic>\n            = 0.001) were dominant in the ascending and descending colon. Site-specific colonization in the gastrointestinal tract may be contributory in the etiology of some diseases of the large intestine.\n          <\/jats:p>","DOI":"10.1128\/aem.01143-07","type":"journal-article","created":{"date-parts":[[2007,9,22]],"date-time":"2007-09-22T00:53:43Z","timestamp":1190422423000},"page":"7435-7442","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":198,"title":["Mucosa-Associated Bacterial Diversity in Relation to Human Terminal Ileum and Colonic Biopsy Samples"],"prefix":"10.1128","volume":"73","author":[{"given":"Shakil","family":"Ahmed","sequence":"first","affiliation":[{"name":"Dundee University Gut Group, Dundee, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George T.","family":"Macfarlane","sequence":"additional","affiliation":[{"name":"Dundee University Gut Group, Dundee, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alemu","family":"Fite","sequence":"additional","affiliation":[{"name":"Dundee University Gut Group, Dundee, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"McBain","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Gilbert","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Macfarlane","sequence":"additional","affiliation":[{"name":"Dundee University Gut Group, Dundee, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.12938\/bifidus1996.19.93"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.56.6.1919-1925.1990"},{"key":"e_1_3_2_5_2","unstructured":"Cancer Statistics registrations; registrations of cancer diagnosed in 2001 2004"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.6.3575-3581.2004"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.4.1587-1594.2000"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2005.00016.x"},{"key":"e_1_3_2_9_2","first-page":"1635","volume":"10","year":"2000","unstructured":"Eckburg, P. 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